Patents by Inventor Thomas KALKBRENNER

Thomas KALKBRENNER has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10401149
    Abstract: A method for capturing image data and for determining the thickness of a specimen holder in the beam path of a microscope. The specimen holder is transparent to illumination radiation and embodied to receive a specimen. The specimen holder, which has a first side face and a second side face, is arranged in a specimen plane and the first side face and second side face of the specimen holder are aligned parallel to the specimen plane. At least one beam of the illumination radiation is directed onto the aligned specimen holder along a first optical axis at a first illumination angle and at least two measured values of a reflected component of the illumination radiation or at least two measurement values of a detection radiation caused by the illumination radiation are captured. Depending on the at least two captured measurement values, a spacing of the first and second side face in relation to one another in the direction of the Z-axis is established as a thickness.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: September 3, 2019
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Jörg Siebenmorgen
  • Publication number: 20190265454
    Abstract: A single plane illumination microscope having an illumination optical system for illuminating a sample located on a sample carrier in a medium, and which is parallel to a planar reference surface. The sample is illuminated by a light sheet via an illumination light path. A detection optical system has a detection beam path. The optical axes of the illumination and detection optical systems each define an angle that is not equal to zero degrees along with the normal to the reference surface. A barrier layer system includes at least one layer of a given material having a given thickness and separates the medium from the illumination and detection optical systems. A base area of the barrier layer system is in contact with the region that is accessible for illumination and detection activities, said base area running parallel to the reference surface.
    Type: Application
    Filed: October 20, 2017
    Publication date: August 29, 2019
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Thomas KALKBRENNER, Ralf NETZ, Helmut LIPPERT, Joerg SIEBENMORGEN
  • Patent number: 10371501
    Abstract: The invention relates to a method for determining height information of a sample, and to a scanning microscope. The method comprises the following steps: generating an illumination spot; illuminating the sample with the illumination spot; capturing an image of a reflection of the illumination spot at the sample; evaluating the lateral distribution of the image; determining the height information from the lateral distribution; wherein the illumination spot has a three dimensional illumination pattern and/or the image in a detection beam path has a three dimensional detection pattern. The scanning microscope is characterized in that an illumination device (07) and/or a detector device comprise(s) a means for generating a three dimensional pattern with a change in the lateral intensity distribution that is asymmetrical along the optical axis, and an evaluation device is configured to determine height information from the detector signal.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: August 6, 2019
    Assignee: Carl Zeiss Microscopy GMBH
    Inventors: Thomas Kalkbrenner, Helmut Lippert
  • Publication number: 20190186895
    Abstract: A method for capturing image data and for determining the thickness of a specimen holder in the beam path of a microscope. The specimen holder is transparent to illumination radiation and embodied to receive a specimen. The specimen holder, which has a first side face and a second side face, is arranged in a specimen plane and the first side face and second side face of the specimen holder are aligned parallel to the specimen plane. At least one beam of the illumination radiation is directed onto the aligned specimen holder along a first optical axis at a first illumination angle and at least two measured values of a reflected component of the illumination radiation or at least two measurement values of a detection radiation caused by the illumination radiation are captured. Depending on the at least two captured measurement values, a spacing of the first and second side face in relation to one another in the direction of the Z-axis is established as a thickness.
    Type: Application
    Filed: December 17, 2018
    Publication date: June 20, 2019
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Thomas KALKBRENNER, Jörg SIEBENMORGEN
  • Publication number: 20190170995
    Abstract: An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen situated on a specimen carrier in a specimen region of a specimen plane via an illumination beam path. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane, in respect of which the specimen carrier is aligned, and the illumination is implemented in the plane. Further, a detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective comprises an illumination correction element arranged in the beam path and/or a detection correction element.
    Type: Application
    Filed: June 29, 2017
    Publication date: June 6, 2019
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Dr. Jörg SIEBENMORGEN, Helmut LIPPERT, Thomas KALKBRENNER, Ingo KLEPPE, Ralf WOLLESCHENSKY, Artur DEGEN, Matthias WALD, Lars-Christian WITTIG, Michael GÖLLES, Wolfgang SINGER
  • Patent number: 10295814
    Abstract: A microscope including an illumination objective with a first optical axis, embodied to produce a light sheet, and a detection objective with a second optical axis, embodied to detect light coming from the specimen plane. The illumination objective and the detection objective are aligned relative to one another and the specimen plane so that the first and second optical axes intersect in the specimen plane and include a substantially right angle therebetween. The optical axes each include an angle which differs from zero with a reference axis directed orthogonal to the specimen plane. An overview illumination apparatus for wide-field illumination of the specimen plane, includes an illumination optical unit with a third optical axis. The characterizing feature is that the detection objective is provided to detect both light from the light sheet and light from the illumination optical unit. A method is also provided for operating a light sheet microscope.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: May 21, 2019
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Jörg Siebenmorgen, Helmut Lippert, Thomas Kalkbrenner
  • Publication number: 20190137742
    Abstract: A microscope having an imaging beam path, an illumination beam path, a detection device, and a control device for controlling the detection device and the illumination device. The control device divides the light sources of the detection device in an array into at least a first and a second group, wherein each group is composed of light sources adjacent to each other in the array and covers part of the array. The control device switches on only one light source of the first group at a point in time and connects the light sources of the first group in a sequence with a clocking in such a way that two light sources switched on one after the other are adjacent to each other in the array and switches the light sources of the second group with the same clocking as the light sources of the first group. The control device reads out the detection device with the same clocking as the connecting of the light sources.
    Type: Application
    Filed: June 2, 2017
    Publication date: May 9, 2019
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Ingo KLEPPE, Thomas KALKBRENNER
  • Publication number: 20190137751
    Abstract: A microscope and method for imaging an object in an object field, the microscope having an illumination device for wide-field illumination of the object. The illumination device has a plurality of light sources, a detection device for recording a wide-field image of the object, and a control device for controlling the detection device and the illumination device. The control device divides the light sources into at least two groups. The light sources of all groups combined fill the object field entirely. The control device for each group switches on all light sources of the group, causes the detection device to record a single image of the object, switches off the light sources of the group, and thus interconnects all groups, and generates a plurality of single images. From the generated single images, an image of the object is generated by the control device.
    Type: Application
    Filed: April 12, 2017
    Publication date: May 9, 2019
    Inventors: Ingo KLEPPE, Thomas KALKBRENNER
  • Publication number: 20190101740
    Abstract: An arrangement and method for supplying immersion media and a method for setting optical parameters of a medium, includes a media supply unit for the controlled supply of a medium or of a mixture into a contact region between an optical lens and a specimen slide, on which a specimen may be arranged. An image capture unit is provided for capturing image data on the basis of detection radiation from the object space along a detection beam path extending through the contact region. An evaluation unit is provided to establish current image parameters on the basis of captured image data, to compare said current image parameters to intended image parameters and to establish a desired mixing ratio of at least two components of the medium depending on the comparison.
    Type: Application
    Filed: September 26, 2018
    Publication date: April 4, 2019
    Applicant: Carl Zeiss Microscopy GmbH
    Inventors: Thomas KALKBRENNER, Sebastian Borck, Jörg Siebenmorgen
  • Patent number: 10247934
    Abstract: A method for examining a specimen via light sheet microscopy includes selecting several illumination wavelengths for the specimen. To structure the illumination light, a predefined phase distribution is impressed on the phase-selective element and a predefined aperture structure is impressed on an aperture in the aperture plane. The phase-selective element is then illuminated in an intermediate image plane in an illumination beam path with illumination light, which is structured by the phase-selective element. The structured illumination light is imaged into an aperture plane arranged downstream of the phase-selective element. The aperture structure is adapted such that the zero orders of the structured illumination light in the aperture plane are substantially removed. The specimen is illuminated with the structured light sheet in the light sheet plane. Light emitted by the specimen is detected in a detection direction which forms an angle different from zero with the light sheet plane.
    Type: Grant
    Filed: November 18, 2015
    Date of Patent: April 2, 2019
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Helmut Lippert, Joerg Siebenmorgen
  • Publication number: 20190094512
    Abstract: The invention relates to an immersion matrix (5), designed for adjusting optical properties at interfaces of optical arrangements, having a porosity with pores of at least one pore size selected from a range from >20 nm to 200 ?m and/or nanopores in the material of the immersion matrix (5), wherein the nanopores have at least one average pore size selected from a range of 0.5 nm to 20 nm, and an elasticity modulus E selected from a range of 0.1-100 MPa. The invention furthermore relates to the use of the immersion matrix (5), an arrangement with the immersion matrix and an immersion device.
    Type: Application
    Filed: September 27, 2018
    Publication date: March 28, 2019
    Inventors: Thomas Ohrt, Thomas Kalkbrenner
  • Patent number: 10234667
    Abstract: A method for evaluating signals of fluorescence scanning microscopy with simultaneous excitation and detection of fluorescence in different focal planes of a sample by means of confocal laser scanning microscopy.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: March 19, 2019
    Assignees: Carl Zeiss Microscopy GmbH, Carl Zeiss AG
    Inventors: Yauheni Novikau, Thomas Kalkbrenner, Tiemo Anhut, Daniel Schwedt, Matthias Wald
  • Publication number: 20190056579
    Abstract: A method for operating a microscopy arrangement, and a microscopy arrangement, having a first microscope and at least one further microscope, wherein each of the microscopes have a respective optical axis. The respective optical axes do not coincide. The method provides a three-dimensional reference coordinate system being set; a carrier apparatus, that is embodied in the arrangement to receive and hold a specimen carrier is introduced into a specimen plane of the first microscope that is intersected by the optical axis and onto the optical axis of the first microscope; a reference point is set on the optical axis of the first microscope; the carrier apparatus is delivered to the further microscope, wherein the current coordinates of the reference point are continuously captured and compared to the coordinates of the optical axis of the at least one further microscope; and the reference point is brought onto the optical axis of the at least one further microscope.
    Type: Application
    Filed: August 14, 2018
    Publication date: February 21, 2019
    Inventors: Thomas Kalkbrenner, Saskia Pergande, Jörg Siebenmorgen, Helmut Lippert
  • Publication number: 20190003967
    Abstract: In a method for creating a digital fluorescent image, the light emitted per pixel from an object plane is converted into a sequence of amplitudes, each of which is associated with one specific measurement time, the sequence of amplitudes is auto-correlated in a manner that is delayed by at least one time offset, and a specific correlation amplitude, from which a total amplitude is determined, is formed for each of the time offsets.
    Type: Application
    Filed: July 27, 2016
    Publication date: January 3, 2019
    Inventors: Thomas KALKBRENNER, Ralf WOLLESCHENSKY
  • Patent number: 10156710
    Abstract: A microscope with at least one illumination beam that is phase modulated in a section along its cross-section with a modulation frequency and a microscope lens for focusing the illumination beam into a test as well as a detection beam path and at least one means of demodulation, wherein at least one polarization altering item is scheduled in the illuminating beam path, for which a phase plate is subordinated that exhibits at least two areas with different phase influence.
    Type: Grant
    Filed: October 3, 2011
    Date of Patent: December 18, 2018
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Tiemo Anhut, Thomas Kalkbrenner, Daniel Schwedt
  • Publication number: 20180341095
    Abstract: A microscope for raster-free, confocal imaging of a sample arranged in a sample space has an illumination arrangement comprising a light source group having light sources which can be switched on individually, a detector arrangement, a pinhole arrangement which comprises a pinhole array and which has a plurality of pinhole elements which are adjacent to one another, wherein there is one pinhole element provided for each light source, and optics which irradiate each pinhole element with radiation of an individual light source of the light source group and confocally illuminate an individual spot located in the sample space, wherein one of the individual spots is associated with each pinhole element, and the individual spots are adjacent to one another in the sample space with respect to an incidence direction of the radiation, and the optics image the individual spots through the pinhole arrangement confocally on the detector arrangement.
    Type: Application
    Filed: May 28, 2018
    Publication date: November 29, 2018
    Applicant: Carl Zeiss Microscopy GmbH
    Inventor: Thomas Kalkbrenner
  • Patent number: 10139611
    Abstract: A method for high-resolution 3D-localization microscopy of a sample having fluorescence emitters, in which the fluorescence emitters are excited to emit fluorescent radiation and the sample is displayed with spatial resolution in wide-field microscopy. Excitation is caused such that at least some fluorescence emitters are isolated. A three-dimensional localization is determined in a localization analysis, which includes a z-coordinate, x-coordinate as well as a y-coordinate orthogonal thereto, for each isolated fluorescence emitter. A table of localization imprecision is provided. Localization imprecision being determined for each localized fluorescence emitter by accessing the table of localization imprecision.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: November 27, 2018
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Yauheni Novikau, Thomas Kalkbrenner
  • Patent number: 10133046
    Abstract: An optical arrangement for being positioned in a beam path of a light microscope, having at least a first and a second optical assembly for providing structured illumination light from incident light. The optical arrangement provides for light to be guided over different beam paths to the various optical assemblies and in the direction of a sample. Electronic control means are provided and designed to illuminate, in each case, a beam path from the different beam paths to different optical assemblies at a point in time, in that at least a first beam combination mirror is provided for guiding light coming from various optical assemblies to a common beam path in the direction of a sample. The first beam combination mirror has reflective areas on which only light from one of the two optical assemblies is incident and has the light-permeable areas of the beam combination mirror in which only light from the other of the optical assemblies is incident.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: November 20, 2018
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Gerhard Krampert, Ralf Netz
  • Publication number: 20180321480
    Abstract: Specimen delimiting element which, at least in one region, has a nano-porous material which is transparent to at least one observation radiation which is capturable by means of a microscope. The nano-porous material has pores, the mean pore diameter of which is smaller than the wavelength of the observation radiation, and a proportion of at least 5% of the volume of the nano-porous material is taken up by the pores at least in portions of said nano-porous material.
    Type: Application
    Filed: October 14, 2016
    Publication date: November 8, 2018
    Inventors: Dr. Thomas OHRT, Thomas KALKBRENNER
  • Patent number: 10119914
    Abstract: A fast, high-resolution localization microscopy method for a specimen containing fluorescence emitters. In a bright state, emitters emit fluorescent radiation upon excitation irradiation, and in a dark state, the fluorescence emitters do not emit fluorescent radiation upon excitation irradiation. The emitters can be brought from the bright state into the dark state by irradiation, and the dark state has a lifetime after which the emitters return to the bright state spontaneously, such that the emitters blink at a blinking frequency. The blinking specimen is imaged with a spatial resolution and detected with a camera, the intensity of the radiation is set such that emitters are isolated in the individual images in terms of the spatial resolution, and the refresh rate at which the images are produced is not lower than the blinking frequency. Locations are determined for isolated emitters in the images with an accuracy that exceeds the spatial resolution.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: November 6, 2018
    Assignee: CARL ZEISS MICROSCOPY GMBH
    Inventor: Thomas Kalkbrenner