Abstract: A microscope having a microscope stand, having at least one microscope objective arranged on the microscope stand, having a holding frame for receiving a sample or a sample carrier and having a sample stage comprising a receiving device for receiving the holding frame. The microscope includes an incubator module that is arrangeable on the holding frame and furthermore wherein the incubator module and the holding frame jointly enclose a sample space at least in part and the holding frame is free from active devices for climate control in the sample space. A system having a plurality of different holding frames and a plurality of different incubator modules for a microscope is described.

Abstract: In a method for checking the rotation of a microscope camera, an overview image is captured with an overview camera of a microscope, wherein a rotational orientation of the overview camera relative to a sample stage of the microscope is known. In addition, a microscope image is captured with a microscope camera of the microscope. A rotational orientation of the microscope camera relative to the sample stage is calculated based on a relative rotation between the microscope image and the overview image, wherein the relative rotation is established by means of image structures in the microscope image and the overview image and using the known rotational orientation of the overview camera relative to the sample stage.

Abstract: A method for repositioning a focus position of an imaging device in a target focus position in a sample in an experiment comprises: defining the target focus position, repositioning a current focus position based on the target focus position, comprising determining one or more compare signatures based on a current focus position, determining one or more distances in each case between the compare signatures and a target signature based on the target focus position, adapting the current focus position based on the distances. A signature is an output of a machine learning model corresponding to a focus position and based on an image of the sample recorded with the focus position, and the target focus position is a focus position in the sample in which the imaging device captures a target image of the sample and the machine learning model outputs the target signature when the target image is input.

Abstract: A microscopy system comprises a microscope with an overview camera for capturing at least one overview image of a sample carrier designed to receive at least one sample fluid; and a computing device configured to determine at least one sample image area of the at least one sample fluid within the at least one overview image. The computing device comprises an evaluation program into which a determined sample image area is entered and which is configured to determine a fluid state of the associated sample fluid based on the sample image area.

Abstract: System for state monitoring of a microscope the system having at least one measuring sensor in each case for capturing at least one time-variable chemical and/or physical quantity, a camera for recording an image in a field of view and a processing unit. The at least one measuring sensor has a display area and displays thereon a measured value for the captured time-variable chemical and/or physical quantity. The camera is arranged so that the display areas of at least one measuring sensor are located in the field of view and the processing unit is configured to evaluate the image and to extract the display areas contained in the image therefrom. Also, a method for state monitoring of a microscope is disclosed, wherein at least one measuring sensor with a display area is provided in order to capture in each case at least one time-variable chemical and/or physical quantity, and an image is recorded. The image is recorded so that it contains the display areas of at least one measuring sensor.

Abstract: A method for preparing a microscope for imaging a sample. The method includes the following steps: providing the microscope for imaging the sample, wherein the microscope comprises an objective having a motor-adjustable objective correction ring for correcting imaging aberrations; acquiring sample information comprising at least one of the following indications: thickness of a cover glass, material of the cover glass, sample temperature, sample type, sample location on a sample carrier, embedding medium of the sample, or immersion medium; imaging the sample using the microscope for generating at least one raw image of the sample with a first setting of the objective correction ring; and inputting the at least one raw image and the sample information into a machine algorithm and determining a second setting of the objective correction ring, which reduces the imaging aberrations vis à vis the first setting, by means of the algorithm on the basis of the raw image and the sample information.

Abstract: A method for ascertaining offset values for correcting the offset of an immersion objective of an image-creating optical system comprises the following steps: imaging an object using a reference objective of the optical system and the immersion objective without immersion and ascertaining offset values for an offset between the reference objective and the immersion objective with immersion on the basis of the image representations of the object

Abstract: For the color correction of microscope images, an object that corresponds to a predetermined object type of a known color is localized in a microscope image using an image processing program. Based on an image region of the localized object, a color correction is determined with which a color of the localized object in the image region is brought into accordance with the known color. The color correction is applied to at least one section of the microscope image or of another microscope image or is used in a capture of a further microscope image.

Abstract: A microscopy system comprises a microscope with an overview camera for capturing at least one overview image of a sample carrier designed to receive at least one sample fluid; and a computing device configured to determine at least one sample image area of the at least one sample fluid within the at least one overview image. The computing device comprises an evaluation program into which a determined sample image area is entered and which is configured to determine a fluid state of the associated sample fluid based on the sample image area.

Abstract: Provision is made for a method of monitoring an immersion fluid in a microscope having a lens which images a sample located on a sample carrier. In a step a), a camera is positioned which has an image field which is oriented in such a way that it captures the sample carrier and a space between the sample carrier and the lens and adjoining the sample carrier towards the lens, which space is used to receive the immersion fluid. In a step b), the immersion fluid is applied into the space between the sample carrier and the lens. In step c), an image with the immersion fluid being in the space between the sample carrier and the lens is recorded, and in a step d), the position, the area and/or the contour of the immersion fluid on the sample carrier from the image recorded in step d) are/is determined.

Abstract: A method for generating an overview image of a sample which is arranged in an observation volume of a microscope by means of a sample carrier is proposed, wherein the sample carrier is illuminated by a first illumination, wherein a preliminary overview image is generated using the first illumination and an overview camera of the microscope, wherein an overview image illumination is chosen on the basis of the preliminary overview image, wherein the sample carrier is illuminated by the overview illumination, and wherein the overview image is generated using the overview image illumination and the overview camera.

Abstract: A method for generating an overview image of a sample which is arranged in an observation volume of a microscope by means of a sample carrier is proposed, wherein the sample carrier is illuminated by a first illumination, wherein a preliminary overview image is generated using the first illumination and an overview camera of the microscope, wherein an overview image illumination is chosen on the basis of the preliminary overview image, wherein the sample carrier is illuminated by the overview illumination, and wherein the overview image is generated using the overview image illumination and the overview camera.

Abstract: A method for processing microscope images comprises: acquiring a microscope image captured by a microscope; identifying at least one image section with sensitive information within the microscope image by means of an image processing program which uses provided reference information regarding sensitive information; rendering unrecognizable the at least one identified image section with sensitive information in order to generate an anonymized image; and outputting the anonymized image.

Abstract: At least one image is obtained which images an external view of at least one interchangeable component (111-116) of an optical system (100). Contextual information for the at least one interchangeable component (111-116) is determined on the basis of the at least one image.

Abstract: System for state monitoring of a microscope the system having at least one measuring sensor in each case for capturing at least one time-variable chemical and/or physical quantity, a camera for recording an image in a field of view and a processing unit. The at least one measuring sensor has a display area and displays thereon a measured value for the captured time-variable chemical and/or physical quantity. The camera is arranged so that the display areas of at least one measuring sensor are located in the field of view and the processing unit is configured to evaluate the image and to extract the display areas contained in the image therefrom. Also, a method for state monitoring of a microscope is disclosed, wherein at least one measuring sensor with a display area is provided in order to capture in each case at least one time-variable chemical and/or physical quantity, and an image is recorded. The image is recorded so that it contains the display areas of at least one measuring sensor.

Abstract: For the color correction of microscope images, an object that corresponds to a predetermined object type of a known color is localized in a microscope image using an image processing program. Based on an image region of the localized object, a color correction is determined with which a color of the localized object in the image region is brought into accordance with the known color. The color correction is applied to at least one section of the microscope image or of another microscope image or is used in a capture of a further microscope image.

Abstract: System for state monitoring of a microscope the system having at least one measuring sensor in each case for capturing at least one time-variable chemical and/or physical quantity, a camera for recording an image in a field of view and a processing unit. The at least one measuring sensor has a display area and displays thereon a measured value for the captured time-variable chemical and/or physical quantity. The camera is arranged so that the display areas of at least one measuring sensor are located in the field of view and the processing unit is configured to evaluate the image and to extract the display areas contained in the image therefrom. Also, a method for state monitoring of a microscope is disclosed, wherein at least one measuring sensor with a display area is provided in order to capture in each case at least one time-variable chemical and/or physical quantity, and an image is recorded. The image is recorded so that it contains the display areas of at least one measuring sensor.

Abstract: A method for regulating a light source of a microscope that illuminates an object, said method including specifying an intended value of an energy parameter of illumination radiation on the object; producing illumination radiation; providing an objective for focusing illumination radiation onto the object; ascertaining a transmission property of the objective for the illumination radiation; output coupling a component of the illumination radiation upstream of the objective as measurement radiation and measuring an actual value of the energy parameter of the measurement radiation; providing a relationship between energy parameters of the measurement radiation and energy parameters of the illumination radiation on the object, and setting the light source in such a way that the actual value of the energy parameter measured for the measurement radiation corresponds to the intended value of the energy parameter according to the relationship.

Abstract: A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective having a front lens enclosed by a surround and being designed for microscopy with an immersion liquid. In the microscope objective, the front lens and/or the surround thereof is provided with a coating which can be switched between a state which repels the immersion liquid and a state which does not repel the immersion liquid.