Abstract: The present invention relates to a method to isolate fully methylated unamplified eukaryotic DNA made of hundreds-of-thousand to billions of base pairs from a lysate. The eukaryotic tissue samples are digested with protolytic enzymes in buffer releasing cellular contents. Magnetically responsive functionalized solid particles are added to the lysate. Polyethylene Glycol and a high salt concentration are added to the mixture to disrupt the hydrogen bonding in the lysate. The eukaryotic genomic DNA binds to the functionalized microparticles and is washed a number of time with an alcohol mixture to remove and denature proteins. The genomic DNA is the eluted off the magnetically responsive functionalized solid particles and used in downstream reactions.

Abstract: A method to directly detect eukaryotic or prokaryotic genomic DNA is disclosed. The invention relates to a method for printing, immobilizing, hybridizing and directly detecting a target nucleic acid sequence in a sample of methylated genomic DNA. Additionally, this invention provides a system for the detection of a target nucleic acid sequence including a flat substrate to bind methylated DNA in discrete patterns and a plurality of labeled target binding probes specific for a target nucleic acid sequence.

Abstract: The present invention provides a method and apparatus for to conduct transgenic and targeted mutagenesis screening of genomic DNA. This invention also provides a system for screening DNA for a designated genetic sequence. The system includes a computer having a processor, memory and web browser, wherein the computer receives instructions concerning the designated genetic sequence and other screening parameter selection from a remote user via a form of electronic communication, and an automatic screening device that analyzes samples of genomic DNA for the designated sequence.