Abstract: A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for accommodating a cell culture insert and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured in the cell culture insert. The means for varying the gas diffusion is a gas permeable membrane provided in an opening in the lid and a removable thin impermeable film label is provided for selectively occluding passage of gases through the gas permeable membrane.

Abstract: A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for accommodating a cell culture insert and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured in the cell culture insert. The means for varying the gas diffusion is a gas permeable membrane provided in a plurality of openings in the lid and a plurality of removable thin impermeable film labels are provided for selectively occluding passage of gases through the gas permeable membranes. The means for minimizing biological contamination of the cells being cultured is a sealing material located in the lid and means for removably securing the lid and the plate together whereby the sealing material is compressed between the lid and the plate.

Abstract: A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for receiving liquids therein and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured. The means for varying the gas diffusion is a gas permeable membrane provided in an opening in the lid and a removable thin impermeable film label is provided for selectively occluding passage of gases through the gas permeable membrane. The means for minimizing biological contamination of the cells being cultured is a sealing material located in the lid and means for removably securing the lid and the plate together whereby the sealing material is compressed between the lid and the plate.

Abstract: An assembly and components therefor are disclosed for use in the field of cell and tissue culture. The assembly includes a base having one or more wells, and a cell culture insert which is mountable to the base such that the bottom wall of the insert is suspended within the well. Each well has a generally rectangular configuration such that openings are provided at each corner of the well for introducing fluid to the well. A cover is provided for protecting the assembly and maintaining the position of the cell culture insert.

Abstract: A vessel assembly for culturing cells wherein a breaker wall zone is provided in the opening of the vessel to minimize media beach or travel into the neck from the vessel body.

Abstract: A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for accommodating a cell culture insert and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured in the cell culture insert. The means for varying the gas diffusion is a gas permeable membrane provided in a plurality of openings in the lid and a plurality of removable thin impermeable film labels are provided for selectively occluding passage of gases through the gas permeable membranes.

Abstract: An assembly and components therefor are disclosed for use in the field of cell and tissue culture. The assembly includes a base having one or more wells, and a cell culture insert which is mountable to the base such that the bottom wall of the insert is suspended within the well. Each well has a generally rectangular configuration such that openings are provided at each corner of the well for introducing fluid to the well. A cover is provided for protecting the assembly and maintaining the position of the cell culture insert.

Abstract: A tissue biopsy cell suspender device includes a receptacle having a sealable opening. The receptacle contains a sufficient quantity of an aqueous medium to support an enzymatic dissociation of cells from a tissue biopsy sample. The device has a removable closure with a first sealing position and a second sealing position for sealing the opening in the receptacle. The device further includes a frangible container having a sufficient quantity of an enzyme therein to dissociate cells from the tissue biopsy sample. The frangible container is covered with a membrane that is permeable to water and to the enzyme. The frangible container is disposed within the receptacle so that the container is intact when the closure is in the first sealing position and the container is breached when the closure is in the second sealing position. When the frangible container is breached, the enzyme is released to permeate into the medium and for dissociation of the cells from the tissue sample.

Abstract: The present invention is a chamber slide assembly having a base member, a cooperable vessel comprising a chamber and a neck wherein said vessel is removably attached to the base and a movable lever attached to the vessel for separating the vessel from the base. Preferably, the assembly may be used for carrying out biological reactions or growth therein, such as growing tissue or cell cultures.

Abstract: The present invention is a culture slide assembly having a base member, a cooperable receptacle chamber removably attached to the base and means for separating the chamber from the base. Preferably, the assembly may be used for carrying out biological reactions or growth therein, such as growing tissue or cell cultures.