Abstract: The present disclosure provides a method for promoting degradation of polychlorinated biphenyls (PCBs) by Pseudomonas JD37 with nanoscale zero-valent iron (nZVI) and use thereof. The method includes the following steps: adding nZVI and Pseudomonas JD37 in an environment having PCBs, and promoting degradation of the PCBs by the Pseudomonas JD37 with the nZVI. The nZVI increases an active oxygen content produced by extracellular metabolism of the Pseudomonas JD37, thus promoting degradation of the PCBs. Therefore, the method of the present disclosure is suitable for degrading the PCBs in water and soil for environmental restoration.

Abstract: Disclosed in the present invention are a pharmaceutical composition comprising a mixture of antibodies of anti-CTLA4 and anti-PD1, a pharmaceutical composition comprising the mixture of antibodies and a chemotherapeutic drug, and use of the above pharmaceutical composition in treating non-small cell lung cancer.

Abstract: The present disclosure discloses a flexible rehabilitation glove based on hybrid actuators. The flexible rehabilitation glove includes five hybrid actuators and an actuator control box, each hybrid actuator includes an actuator end mounting seat, a flexible actuator, a TPFE water pipe, a flexible actuator front section mounting seat, a flexible actuator air pipe, an air pipe connecting seat II, an SMA spring actuator, an air pipe connecting seat I and a cooling water pipe, the control box includes an air pump, a filter, a water pump and a water tank, a proportional valve and a control circuit board inside, and includes an actuator control box body, a control box cover, a main switch, a button and a voltage display outside, and the filter is connected to the air pump and the electrical proportional valve respectively.

Abstract: Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.

Abstract: Principles for reliable manufacturing carbon-based electrodes with arbitrary sensitivity and homogeneity are provided. Specifically, the sensitivity of carbon-based electrodes can be engineered by changing the density of sp2 type defects present in a multilayer graphene film. The engineered carbon-based electrodes can be used as a passive sensing element in electrochemical measurement of a target analyte. Carbon-based electrodes are also disclosed that have sp2 hybridization and can include multilayer graphene films. The disclosed carbon-based electrodes have a density of zero-dimensional defects (i.e., point-like defects) which provides enhanced area-normalized sensitivity when used in sensing applications. The maximum area-normalized sensitivity is achieved at the point defect density of 4-5×1012 cm?2.

Abstract: Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.

Abstract: The disclosure provides an InDel marker genotype database of whole genome of Malus and use in specific identification of germplasm resources thereof, belonging to the technical field of molecular biology. The constructing method of the genotype database comprises: genotyping InDel markers of genomic DNA samples of Malus germplasm resources by using primer pairs and multiplex PCR; recording one line data for each Malus germplasm resource according to the genotype of InDel marker; collecting all germplasm resources records to obtain an InDel marker genotype database of the whole genome of Malus. The whole genome InDel marker genotype database of Malus provided by the disclosure can identify the specificity of apple germplasm simply, efficiently and stably, thus overcoming the limitation that DUS test is easily influenced by environment, and providing a powerful tool for the protection of new apple varieties and the management of germplasm resources.

Abstract: The present disclosure provides a method for promoting degradation of polychlorinated biphenyls (PCBs) by Pseudomonas JD37 with nanoscale zero-valent iron (nZVI) and use thereof. The method includes the following steps: adding nZVI and Pseudomonas JD37 in an environment having PCBs, and promoting degradation of the PCBs by the Pseudomonas JD37 with the nZVI. The nZVI increases an active oxygen content produced by extracellular metabolism of the Pseudomonas JD37, thus promoting degradation of the PCBs. Therefore, the method of the present disclosure is suitable for degrading the PCBs in water and soil for environmental restoration.

Abstract: Disclosed is a method for detection a malicious attack based on deep learning in a transportation cyber-physical system (TCPS), comprising: extracting original feature data of a malicious data flow and a normal data flow from a TCPS; cleaning and coding original feature data; selecting key features from the feature data; cleaning and coding the key features to establish a deep learning model; finally, inputing unknown behavior data to be identified into the deep learning model to identify whether the data is malicious data, thereby detecting a malicious attack. The present invention uses a deep learning method to extract and learn the behavior of a program in a TCPS, and detect the malicious attack according to the deep learning result, and effectively identify the malicious attack in the TCPS.

Abstract: A strain of Oceanobacillus picturae and applications thereof are provided, which relate to technical fields of microorganism and food processing. A strain of halophilic bacteria was screened from the brine of Xiangyang Pickled Kohlrabi and was identified as the strain of Oceanobacillus picturae by morphology, physical and chemical properties and 16S rRNA sequence. The strain is able to significantly increase the varieties and contents of volatile substances, and decrease the contents of pungent substances in pickled vegetables. The strain of Oceanobacillus picturae can improve the taste and the flavor of the pickled vegetables.

Abstract: A rehabilitation glove based on a bidirectional driver of a honeycomb imitating structure, including five bidirectional drivers and a cotton glove. The drivers are fixed to a back of the glove through hook and loop fasteners. Each driver includes a hollow buckling air bag in a continuous bent state, a middle guide layer in a continuous bent state and a hollow stretching air bag. The buckling air bag and the middle guide layer are symmetrically arranged, and the stretching air bag in a straightened state is arranged below the middle guide layer. A novel bidirectional driver of a honeycomb imitating structure is provided, which may provide a patient with rehabilitation training in two degrees of freedom: buckling and stretching. A control algorithm of the bidirectional driver is further provided to perform force control output for the driver, which may better help the patient recover hand functions.

Abstract: The disclosure relates to an anti-VEGF-PD1 bispecific antibody with a novel structure and a use thereof, which belongs to the technical field of molecular immunology. The CDR-H1 in the heavy chain variable region of the antibody is an amino acid sequence expressed by SEQ ID NO: 1, the CDR-H2 is an amino acid sequence expressed by SEQ ID NO: 2, the CDR-H3 is an amino acid sequence expressed by SEQ ID NO: 3, and the CDR-L in the light chain variable region of the antibody is an amino acid sequence expressed by SEQ ID NO:4.

Abstract: A rehabilitation glove based on a bidirectional driver of a honeycomb imitating structure, including five bidirectional drivers and a cotton glove. The drivers are fixed to a back of the glove through hook and loop fasteners. Each driver includes a hollow buckling air bag in a continuous bent state, a middle guide layer in a continuous bent state and a hollow stretching air bag. The buckling air bag and the middle guide layer are symmetrically arranged, and the stretching air bag in a straightened state is arranged below the middle guide layer. A novel bidirectional driver of a honeycomb imitating structure is provided, which may provide a patient with rehabilitation training in two degrees of freedom: buckling and stretching. A control algorithm of the bidirectional driver is further provided to perform force control output for the driver, which may better help the patient recover hand functions.

Abstract: The present disclosure provides a genomics-assisted prediction method for apple fruit quality traits and disease resistance and use thereof, and belongs to the technical field of plant genetics, genomics and breeding. The method is achieved by molecular markers. There are a total of 319 molecular markers, including 318 single-nucleotide polymorphism (SNP) markers and 1 InDel marker; and the molecular markers are associated with 16 traits of the Malus genus, including fruit ripening date, fruit shape, fruit cover color degree, fruit weight, soluble solid content, fruit juice pH value, malate content, chlorogenate content, procyanidin B2 content, flesh firmness at harvest, flesh crispness at harvest, flesh firmness retainability, flesh crispness retainability, fruit ring rot disease resistance, Glomerella leaf blotch resistance, and spur tree architecture.

Abstract: A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.

Abstract: Provided are a CAR-T cell specifically recognizing CD70, and a preparation method therefor. The method comprises expressing a SCFV(Anti CD70)-CD8-4-1BB-CD3? molecule in a T cell. The CAR-T cell has tumor killing activity and may be used for preparing a medication in the treatment of a tumor with the high expression level of CD70 molecules.

Abstract: T cells are transfected with a recombinant RNA molecule that encodes at least one of an alpha chain and a beta chain of a CD1b restricted T cell receptor. Preferably, the so prepared T cells are used as a cell-based therapeutic composition to treat tuberculosis.

Abstract: The present disclosure discloses melatonin derivatives, a method thereof, and a use thereof. The method comprises: dissolving melatonin in a solvent, adding a catalyst while maintaining the solvent at 0° C. to obtain a first reaction solution, stirring the first reaction solution, adding reactants in the first reaction solution, continually stirring at room temperature or at refluxing reaction temperature until, when analyzing by thin layer chromatography, the first reaction solution is fully reacted, quenching the reaction with ice water, concentrating the first reaction solution to obtain a residue of the first reaction solution, and purifying the residue of the first reaction solution by column chromatography to obtain the melatonin derivatives.

Abstract: The disclosure provides an InDel marker genotype database of whole genome of Malus and use in specific identification of germplasm resources thereof, belonging to the technical field of molecular biology. The constructing method of the genotype database comprises: genotyping InDel markers of genomic DNA samples of Malus germplasm resources by using primer pairs and multiplex PCR; recording one line data for each Malus germplasm resource according to the genotype of InDel marker; collecting all germplasm resources records to obtain an InDel marker genotype database of the whole genome of Malus. The whole genome InDel marker genotype database of Malus provided by the disclosure can identify the specificity of apple germplasm simply, efficiently and stably, thus overcoming the limitation that DUS test is easily influenced by environment, and providing a powerful tool for the protection of new apple varieties and the management of germplasm resources.