Abstract: An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Abstract: An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Abstract: Methods and devices are provided to provide clean signals even in the presence of spectral interference. At least some of these methods can be applied for cases when interfering signals are to be accounted for. These cases include, but are not limited to, hemolysis detection, icterus detection, and assays. They can be implemented in with data collected with spectrophotometers, instruments that can collect absorbance values at the few wavelengths of interest, and, in the case of the method based on background subtraction, simple imaging setups with only two filters (such as but not limited to narrow-band and wide-band) per absorption peak of interest.

Abstract: An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Abstract: Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Abstract: An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Abstract: Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Abstract: Methods and devices are provided to provide clean signals even in the presence of spectral interference. At least some of these methods can be applied for cases when interfering signals are to be accounted for. These cases include, but are not limited to, hemolysis detection, icterus detection, and assays. They can be implemented in with data collected with spectrophotometers, instruments that can collect absorbance values at the few wavelengths of interest, and, in the case of the method based on background subtraction, simple imaging setups with only two filters (such as but not limited to narrow-band and wide-band) per absorption peak of interest.

Abstract: Credentials and other sensitive strings can undergo automatic rotation before each transmission or storage of those credentials. String modification, which can utilize a key stretching algorithm, can be used to modify the credential before transmission. This can be for an initial sign up, a subsequent login, or another such action. A random number can be generated to determine the number of iterations for the key stretching algorithm to be applied. For subsequent actions, a new random number can be added to the prior iteration number in order to create a new string that can be generated using the previously utilized iteration number and the new random number, with only the new random number being transmitted with the modified credential string. This increases security, as the transmission itself cannot be used to recover the original plaintext credential if recovered.

Abstract: An embodiment of a system includes a compartment-generating device, a compartment detector, and electronic computing circuitry. The device is configured to generate compartments of a digital assay, at least one of the compartments having a respective volume that is different from a respective volume of each of at least another one of the compartments. The detector is configured to determine a number of the compartments each having a respective number of a target that is greater than a threshold number of the target. And the electronic circuitry is configured to determine a bulk concentration of the target in a source of the sample in response to the determined number of compartments. Because such a system can be configured to estimate a bulk concentration of a target in a source from a polydisperse digital assay, the system can be portable, and lower-cost and faster, than conventional systems.

Abstract: Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.

Abstract: Methods and devices are provided to provide clean signals even in the presence of spectral interference. At least some of these methods can be applied for cases when interfering signals are to be accounted for. These cases include, but are not limited to, hemolysis detection, icterus detection, and assays. They can be implemented in with data collected with spectrophotometers, instruments that can collect absorbance values at the few wavelengths of interest, and, in the case of the method based on background subtraction, simple imaging setups with only two filters (such as but not limited to narrow-band and wide-band) per absorption peak of interest.

Abstract: Credentials and other sensitive strings can undergo automatic rotation before each transmission or storage of those credentials. String modification, which can utilize a key stretching algorithm, can be used to modify the credential before transmission. This can be for an initial sign up, a subsequent login, or another such action. A random number can be generated to determine the number of iterations for the key stretching algorithm to be applied. For subsequent actions, a new random number can be added to the prior iteration number in order to create a new string that can be generated using the previously utilized iteration number and the new random number, with only the new random number being transmitted with the modified credential string. This increases security, as the transmission itself cannot be used to recover the original plaintext credential if recovered.

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract: Credentials and other sensitive strings can undergo automatic rotation before each transmission or storage of those credentials. String modification, which can utilize a key stretching algorithm, can be used to modify the credential before transmission. This can be for an initial sign up, a subsequent login, or another such action. A random number can be generated to determine the number of iterations for the key stretching algorithm to be applied. For subsequent actions, a new random number can be added to the prior iteration number in order to create a new string that can be generated using the previously utilized iteration number and the new random number, with only the new random number being transmitted with the modified credential string. This increases security, as the transmission itself cannot be used to recover the original plaintext credential if recovered.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.

Abstract: Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract: A file system data is divided into two or more data blocks. A unique encryption key is assigned to each data block with the encryption key assigned to each data block being distinct from other encryption keys used to encrypt the other data blocks and each of the data blocks is encrypted using its assigned encryption key. One of the data blocks within the file system is then selected and decrypted using the distinct encryption key assigned to the selected data block and a new encryption key, distinct for the previously assigned encryption key, is assigned to the selected data block and the selected data block is re-encrypted using the new encryption key. This process is then repeated for each data block on a sequential/cyclic and continually rotating basis.

Abstract: A method for biometric identification for use with a computing device is provided herein. The method includes capturing a temporal sequence of images of the face of a user at different locations within a three-dimensional interaction space. The method further includes extracting one or more face descriptors from the images and generating a biometric template compiling the face descriptors.