Abstract: The present invention relates to a therapeutic oligonucleotide having a 5? and a 3? end and comprising a sequence of nucleosides linked together by inter-nucleoside linkages, wherein: at least one inter-nucleoside linkage is an amide linker moiety; at least one inter-nucleoside linkage is a phosphorothioate linker moiety; and at least one nucleoside present in the oligonucleotide is a locked nucleoside; wherein the at least one locked nucleoside is directly attached to the 3? end or the 5? end of the amide linker moeity.

Abstract: Provided herein is a method of producing a polynucleotide or an analogue or derivative thereof, comprising providing a support-attached nucleoside, nucleotide, polynucleotide or derivative or analogue thereof, which is a compound of formula (I) as defined herein and attached to a solid support at (II), (ii) increasing the chain length of the support-attached nucleoside, nucleotide, polynucleotide or derivative or analogue thereof by introducing one or more additional nucleoside units, nucleotide units and/or analogues or derivatives thereof; and (iii) cleaving the resulting polynucleotide from the solid support by photo-illuminating the compound of formula (I). Also provided are associated compounds, uses, and polynucleotides obtainable by such methods.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: Methods and systems for providing content and content recommendations are described. Content recommendations for users in a service area may be based on content requests from users within the same service area. Content recommendations over a period of time may dynamically change based on many factors, including: the number of requests for particular content within one or more time frames, the storage location of requested content relative to requesting users, and whether a request occurs within a time period of rapid shift in use and types of content requested. A content recommendation including one or more content recommendations may be provided to users.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: Methods and systems for providing content and content recommendations are described. Content recommendations for users in a service area may be based on content requests from users within the same service area. Content recommendations over a period of time may dynamically change based on many factors, including: the number of requests for particular content within one or more time frames, the storage location of requested content relative to requesting users, and whether a request occurs within a time period of rapid shift in use and types of content requested. A content recommendation including one or more content recommendations may be provided to users.

Abstract: The invention relates to methods of adding identifier sequences to polynucleotides of an array. The identifier sequences comprise a plurality of nucleotide blocks. Also provided are arrays of polynucleotides having identifier sequences, microparticles comprising said arrays, a plurality of 5 said microparticles, surfaces comprising said arrays, kits and methods for generating libraries using the array, methods for determining the accuracy of sequencing or amplification an array, and methods of analysing said libraries.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: Systems and methods are described that relate to on-demand compression for electronic data, such as media content, through a communication pathway. The communication pathway may be configured to multicast media content to a plurality of end-users in a service group. In certain embodiments, the service group may comprise a service group node having a quadrature amplitude modulation device. Optimization techniques may be applied a network including the communication pathway and/or to the media content. In some examples, it may be determined whether a bandwidth utilization threshold is satisfied. The determination may be based on, at least in part, by measurements taken at one or more electronic devices located throughout a network or system. If the threshold is not met, the network and/or the media content may be optimized using a first technique. If the threshold is met, the network and/or the media content may be optimized using a second technique.

Abstract: Systems and methods are described that relate to on-demand compression for electronic data, such as media content, through a communication pathway. The communication pathway may be configured to multicast media content to a plurality of end-users in a service group. In certain embodiments, the service group may comprise a service group node having a quadrature amplitude modulation device. In one exemplary method, it may be determined whether the amount of media content being transmitted meets a first utilization threshold. The determination may be based on, at least in part, by measurements taken at one or more electronic devices located throughout a network or system. If the first bandwidth utilization threshold is not met, media content may be transmitted in a first format. If the first bandwidth utilization threshold is met, at least a portion of the media content may be transmitted in a first optimized delivery technique.

Abstract: The invention relates to micro-particles in which polynucleotides are joined to a bead at the 3? end and include a linker that can be cleaved to separate the polynucleotides from the bead and provide free 3? hydroxyl groups. Also provided are arrays of polynucleotides, pluralities of micro-particles, fluidic compartments comprising micro-particles, methods of synthesising the arrays and methods of generating libraries using the array.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: Systems and methods are described that relate to on-demand compression for electronic data, such as media content, through a communication pathway. The communication pathway may be configured to multicast media content to a plurality of end-users in a service group. In certain embodiments, the service group may comprise a service group node having a quadrature amplitude modulation device. In one exemplary method, it may be determined whether the amount of media content being transmitted meets a first utilization threshold. The determination may be based on, at least in part, by measurements taken at one or more electronic devices located throughout a network or system. If the first bandwidth utilization threshold is not met, media content may be transmitted in a first format. If the first bandwidth utilization threshold is met, at least a portion of the media content may be transmitted in a first optimized delivery technique.

Abstract: Methods are described for managing the delivery of electronic data, such as media content, through a network. An indication of a quantity of data transmitted via a network during a first time period may be received, the quantity of data including a first portion of a content item. Based on the quantity of data transmitted during the first time period, it may be determined that a first bandwidth utilization threshold of the network has been satisfied, and the network may be optimized for delivery of a second portion of the content item.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: Cache management techniques are described for a content distribution network (CDN), for example, a video on demand (VOD) system supporting user requests and delivery of video content. A preferred cache size may be calculated for one or more cache devices in the CDN, for example, based on a maximum cache memory size, a bandwidth availability associated with the CDN, and a title dispersion calculation determined by the user requests within the CDN. After establishing the cache with a set of assets (e.g., video content), an asset replacement algorithm may be executed at one or more cache devices in the CDN. When a determination is made that a new asset should be added to a full cache, a multi-factor comparative analysis may be performed on the assets currently residing in the cache, comparing the popularity and size of assets and combinations of assets, along with other factors to determine which assets should be replaced in the cache device.

Abstract: Systems and methods for generating and receiving a sequenced listing of pieces of content are described. A request to search for assets may be received. A first listing of assets associated with the request may be received. Data representative of assets stored within an edge server associated with the user may be received. A first group of assets of the plurality of assets that are stored within the edge server based upon the received data representative of assets stored within the edge server and a second group of assets of the plurality of assets that are not stored within the edge server based upon the received data representative of assets stored within the edge server may be determined. A second listing of assets may be generated where the first group of assets is sequenced earlier in the second listing than the second group of assets.

Abstract: Systems and methods for generating and receiving a sequenced listing of pieces of content are described. A request to search for assets may be received. A first listing of assets associated with the request may be received. Data representative of assets stored within an edge server associated with the user may be received. A first group of assets of the first listing of assets that are stored within the edge server based upon the received data representative of assets stored within the edge server and a second group of assets of the first listing of assets that are not stored within the edge server based upon the received data representative of assets stored within the edge server may be determined. A second listing of assets may be generated where at least one asset of the first group is sequenced separate from at least one asset of the second group.

Abstract: The present disclosure relates to novel dinucleotides comprising at least two locked nucleosides, one of which is directly attached to the 3? end of the triazole linker moiety and the other of which is directly linked to the 5? end of the triazole linker moiety and that are useful for the preparation of oligonucleotides. The disclosed dinucleotides may be used in gene therapy.