Abstract: Cache management techniques are described for a content distribution network (CDN), for example, a video on demand (VOD) system supporting user requests and delivery of video content. A preferred cache size may be calculated for one or more cache devices in the CDN, for example, based on a maximum cache memory size, a bandwidth availability associated with the CDN, and a title dispersion calculation determined by the user requests within the CDN. After establishing the cache with a set of assets (e.g., video content), an asset replacement algorithm may be executed at one or more cache devices in the CDN. When a determination is made that a new asset should be added to a full cache, a multi-factor comparative analysis may be performed on the assets currently residing in the cache, comparing the popularity and size of assets and combinations of assets, along with other factors to determine which assets should be replaced in the cache device.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: The present disclosure describes a method and system for monitoring the bandwidth utilization on a high speed data network port over time. According to the method described herein, measurements of the bandwidth utilization on the port may be taken over a predetermined time interval and analyzed to determine whether the utilization measurement equals or exceeds a predetermined upper threshold. If the port exhibits high bandwidth utilization within the time interval, an alert may be set for further monitoring of the port or for port augmentation.

Abstract: A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm.

Abstract: Systems and methods for generating and receiving a sequenced listing of pieces of content are described. A request to search for assets may be received. A first listing of assets associated with the request may be received. Data representative of assets stored within an edge server associated with the user may be received. A first group of assets of the first listing of assets that are stored within the edge server based upon the received data representative of assets stored within the edge server and a second group of assets of the first listing of assets that are not stored within the edge server based upon the received data representative of assets stored within the edge server may be determined. A second listing of assets may be generated where at least one asset of the first group is sequenced separate from at least one asset of the second group.

Abstract: Systems and methods are discussed for providing a sensor enhanced employee safety evaluation system. Sensors that monitor employee behavior are placed at the workplace of an insured entity. Data from the sensors are processed to obtain a safety evaluation. Based on the safety evaluation, the insurance provider can adjust the terms of the insurance policy to accurately reflect the risks associated with the insured entity. Feedback based on the safety evaluation is also provided to the insured entity and the employees of the insured entity to promote improvements in safe behavior.

Abstract: A method for determining the number of tandem repeats in a target polynucleotide, the method comprising (a) providing a sample containing the target polynucleotide, wherein one or more of the tandem repeats in the target polynucleotide is in single stranded form, (b) hybridising a labelled probe oligonucleotide to the single stranded portion of the target polynucleotide, wherein the probe oligonucleotide is complementary to at least one of the tandem repeats, and at least 5 nucleotides of the probe oligonucleotide are complementary to the tandem repeats, in the single stranded portion of the target polynucleotide, and (c) determining the number of tandem repeats in the target polynucleotide based on the hybridisation of the probe oligonucleotide to the single stranded portion of the target polynucleotide.

Abstract: The invention provides a hairpin polynucleotide, having a loop and a stem region, characterised in that a sulfur-based nucleophile is attached to an internal nucleotide in the hairpin through a linker to enable binding to a solid support.

Abstract: A structural post arrangement and cooperating clamp assembly positioning and supporting related furniture components, such as beams. The clamping assembly includes a split clamping collar which surrounds the post and defines upper and lower rims for engagement with top and bottom jaws as coupled to a furniture component.

Abstract: The use of a red nucleic acid stain, in particular red fluorescent SYTO® dye in various methods used for the detection or characterisation of nucleic acids is described. In particular, the red nucleic acid stains have been found to be particularly compatible with the polymerase chain reaction (PCR), and therefore form the basis of enhanced detection methods.

Abstract: A door assembly comprising an outer door frame and a panel mounted in a panel frame, the door assembly further comprising a first outer door skin comprising a body portion of a defined first thickness and defining an aperture for receiving the panel. A first stepped portion adjacent a periphery of the aperture, the first stepped portion comprising a defined second thickness and adapted to receive the panel frame of the panel therein. The panel frame of the panel is received within the first stepped portion and the first thickness of the body portion is greater than the second thickness of the first stepped portion.

Abstract: A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm.

Abstract: Systems and methods are discussed for providing a sensor enhanced employee safety evaluation system. Sensors that monitor employee behavior are placed at the workplace of an insured entity. Data from the sensors are processed to obtain a safety evaluation. Based on the safety evaluation, the insurance provider can adjust the terms of the insurance policy to accurately reflect the risks associated with the insured entity. Feedback based on the safety evaluation is also provided to the insured entity and the employees of the insured entity to promote improvements in safe behavior.

Abstract: A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with a reporter molecule preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm.

Abstract: The invention provides a hairpin polynucleotide, having a loop and a stem region, characterised in that a sulfur-based nucleophile is attached to an internal nucleotide in the hairpin through a linker to enable binding to a solid support.

Abstract: A structural post arrangement and cooperating clamp assembly positioning and supporting related furniture components, such as beams. The clamping assembly includes a split clamping collar which surrounds the post and defines upper and lower rims for engagement with top and bottom jaws as coupled to a furniture component.