Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

Abstract: The present invention provides a separation column including a monolithic rod without a resin coating material. The separation column can prevent the monolithic rod from destroying, and a liquid can be delivered at a high pressure into the separation column. The separation column includes the monolithic rod (monolithic silica rod) in a tapered-columnar shape, and a clad with the interior formed in a tapered-columnar shape. The monolithic rod is built into the clad.

Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.

Abstract: In a capillary electrophoresis apparatus, a capillary array unit has a capillary array including capillaries having a capillary head and a detection unit, a frame for supporting the capillary array, and a load header for holding cathode ends of the capillaries. The frame has separators for separating and holding the capillaries. The capillary head, the detection unit, and the separators are disposed along one linear line. With this arrangement, there is provided the capillary array unit and the capillary electrophoresis apparatus which are arranged such that a job for mounting the capillary array can be executed easily.

Abstract: At a wall constituting a space for a thermostatic oven in an electrophoresis apparatus, a capillary array attachment portion is formed which permits attachment of a plurality of capillary arrays having different length. Thereby, a selected capillary array constituted by collecting a plurality of capillaries can be easily attached to the electrophoresis apparatus depending on measurement purpose.

Abstract: Even if a standby time to the measurement becomes long, a sample in a sample container is prevented from deteriorating. A cooling mechanism is provided in a sample tray for holding a sample plate, or in a plate-mounting portion of a table-type auto sampler of a capillary electrophoresis apparatus, so that the sample plate is cooled during the measurement also.

Abstract: To obtain a stable pressure to inject polymer into a capillary array in order to shorten the injection time and raise the processing capacity of the apparatus. Disclosed herein is an electrophoresis apparatus comprising one or more capillaries to be filled with an electrophoretic medium and a pump mechanism which can be connected to the capillaries for filling the capillaries with an electrophoretic medium. The pump mechanism comprises a plunger; a slider which is bonded to the plunger and moves together with the plunger; a spring which gives force to the slider; a cover which limits the range of displacement of the slider; and a linear motion system which is bonded to the cover and moves linearly, wherein the cover and the slider move as one body when the electrophoretic medium is taken to the plunger, and the plunger is pushed by the action of the spring when the electrophoretic medium is injected from the plunger into the capillaries.

Abstract: A monolithic separation column and a liquid chromatography system using the same are provided by which the pressure tightness is improved, and the manufacturing is enabled at a low temperature, thus preventing the degradation of the separation performance. A monolithic rod 118 is made of a porous body and has a cylindrical shape. A coat material 116 coats an outer circumference of the monolithic rod 118 and includes an uneven portion 116R on an outer face thereof A holder 112, into which the monolithic rod 118 coated with the coat material 116 is inserted, includes a taper portion 112T on an inner face thereof. A potting media 114 is fitted or filled between the coat material 116 and the holder 112.

Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.

Abstract: An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.

Abstract: The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased. An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.

Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Abstract: An electrophoresis apparatus is provided in which negative effects caused by abnormalities in a current-carrying path in an electrophoresis apparatus can be avoided or reduced. The current flowing in the current-carrying path during electrophoresis can be measured to detect the state of a separating medium, and the application of a voltage to the current-carrying path can be interrupted.

Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

Abstract: At a wall constituting a space for a thermostatic oven in an electrophoresis apparatus, a capillary array attachment portion is formed which permits attachment of a plurality of capillary arrays having different length. Thereby, a selected capillary array constituted by collecting a plurality of capillaries can be easily attached to the electrophoresis apparatus depending on measurement purpose.

Abstract: At a wall constituting a space for a thermostatic oven in an electrophoresis apparatus, a capillary array attachment portion is formed which permits attachment of a plurality of capillary arrays having different length. Thereby, a selected capillary array constituted by collecting a plurality of capillaries can be easily attached to the electrophoresis apparatus depending on measurement purpose.