Patents by Inventor Tomoko Matsui
Tomoko Matsui has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8507240Abstract: The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.Type: GrantFiled: May 16, 2012Date of Patent: August 13, 2013Assignee: Novozymes A/SInventors: Soeren Flensted Lassen, Leonardo De Maria, Lars Kobberoee Skov, Esben Peter Friis, Tomoko Matsui, Allan Noergaard, Jesper Vind
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Publication number: 20130195829Abstract: The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.Type: ApplicationFiled: March 25, 2011Publication date: August 1, 2013Applicant: NOVOZYMES A/SInventors: Lars Koberoee Skov, Leonardo De Maria, Tomoko Matsui
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Publication number: 20120330493Abstract: A method, featuring robustness against changes in tire size, is provided for determining a road surface condition by dividing a time-series waveform of tire vibrations into windows without resorting to detection of the peak positions or measurement of the wheel speed. A time-series waveform of tire vibrations detected by a tire vibration detecting unit is windowed by a windowing unit. Time-series waveforms are extracted from the respective time windows, feature vectors X are calculated therefor, and then likelihoods Z for road-surface HMMs (hidden Markov models) are calculated. The likelihoods Z1 to Z5 calculated for the respective road-surface HMMs are compared with one another, and a road surface condition corresponding to the road-surface HMM showing the highest likelihood is determined to be the condition of the road surface on which the tire is running.Type: ApplicationFiled: June 20, 2012Publication date: December 27, 2012Applicants: Inter-University Research Institute Corporation, Research Organization of Information and System, KABUSHIKI KAISHA BRIDGESTONEInventors: Yasushi HANATSUKA, Tomoyuki HIGUCHI, Tomoko MATSUI
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Publication number: 20120309067Abstract: The present invention relates to proteases having at least 75% identity to a protease derived from Thermoascus aurantiacus and comprises at least one modification in the amino acid sequence thereof. These protease variants have improved thermostability. The invention also relates to DNA encoding these proteases, methods of their production, as well as the use thereof.Type: ApplicationFiled: December 10, 2010Publication date: December 6, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Tomoko Matsui, Allan Noergaard, Thomas Poulsen Agersten, John Matthews
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Publication number: 20120294978Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: ApplicationFiled: July 19, 2012Publication date: November 22, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielson, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Patent number: 8263381Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: GrantFiled: August 20, 2010Date of Patent: September 11, 2012Assignees: Novozyms A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Publication number: 20120225468Abstract: The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.Type: ApplicationFiled: May 16, 2012Publication date: September 6, 2012Applicant: NOVOZYMES A/SInventors: Soeren Flensted Lassen, Leonardo De Maria, Esben Peter Friis, Tomoko Matsui, Allan Noergaard, Lars Kobberoee Skov, Jesper Vind
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Patent number: 8206962Abstract: The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.Type: GrantFiled: September 28, 2009Date of Patent: June 26, 2012Assignee: Novozymes A/SInventors: Soeren Flensted Lassen, Leonardo De Maria, Esben Peter Friis, Tomoko Matsui, Allan Noergaard, Lars Kobberoee Skov, Jesper Vind
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Publication number: 20120122157Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: ApplicationFiled: January 24, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Patent number: 8124379Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: GrantFiled: March 31, 2009Date of Patent: February 28, 2012Assignee: Novozymes A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Publication number: 20110104759Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: ApplicationFiled: December 20, 2010Publication date: May 5, 2011Applicants: Novozymes A/S, Novozymes North America, Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Patent number: 7883883Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: GrantFiled: June 25, 2004Date of Patent: February 8, 2011Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Patent number: 7871806Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.Type: GrantFiled: May 18, 2010Date of Patent: January 18, 2011Assignee: Novozymes A/SInventors: Tomoko Matsui, Esben Peter Friis, Akihiko Yamagishi
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Publication number: 20100323061Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: ApplicationFiled: August 20, 2010Publication date: December 23, 2010Applicants: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Eric Allain, Carsten Hjort, Anders Vikso-Nielsen, Shiro Fukuyama, Tomoko Matsui
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Patent number: 7829320Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.Type: GrantFiled: November 6, 2009Date of Patent: November 9, 2010Assignee: Novozymes A/SInventors: Tomoko Matsui, Esben Peter Friis, Akihiko Yamagishi
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Publication number: 20100261259Abstract: The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.Type: ApplicationFiled: August 3, 2007Publication date: October 14, 2010Applicant: Novozymes A/SInventors: Tomoko Matsui, Pia Francke Johannesen, Shinobu Takagi, Thomas Agersten Poulsen, Noriko Tsutsumi, Zheng Liu, XiangYu Kong
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Publication number: 20100221385Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.Type: ApplicationFiled: May 18, 2010Publication date: September 2, 2010Applicant: NOVOZYMES A/SInventors: TOMOKO MATSUI, ESBEN PETER FRIIS, AKIHIKO YAMAGISHI
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Publication number: 20100180095Abstract: The buffer control device of this invention includes: a pointer holding unit which holds a virtual pointer different from a read pointer and a write pointer; an access control unit that controls an access to a ring buffer; a judging unit that judges whether or not one of the read pointer and the write pointer has reached an address substantially identical to an address indicated by the virtual pointer; and disabling unit that disables a normal access using the one of the read pointer and the write pointer, when the judging unit judges that the one of the read pointer and the write pointer has reached the address substantially identical to the address indicated by the virtual pointer, the normal access being controlled by the access control unit, wherein the access control unit further controls a reaccess to the ring buffer.Type: ApplicationFiled: November 28, 2006Publication date: July 15, 2010Applicant: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.Inventors: Masanori Fujibayashi, Nobuo Higaki, Kazushi Kurata, Tomoko Matsui
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Publication number: 20100083392Abstract: The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.Type: ApplicationFiled: September 28, 2009Publication date: April 1, 2010Applicant: Novozymes A/SInventors: Soeren Flensted Lassen, Leonardo De Maria, Esben Peter Friis, Tomoko Matsui, Allan Noergaard, Lars Kobberoee Skov, Jesper Vind
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Publication number: 20100055726Abstract: The invention relates to new asparaginases having improved properties, preferably improved thermotolerance, such as improved activity at high temperatures and/or improved thermostability. The invention also relates to DNA sequences encoding such improved asparaginases, their production in a recombinant host cell, as well as methods of using the asparaginases, in particular for reduction of acrylamide in foods. The invention furthermore relates to methods of generating and preparing asparaginase variants having improved properties.Type: ApplicationFiled: November 6, 2009Publication date: March 4, 2010Applicant: NOVOZYMES A/SInventors: TOMOKO MATSUI, ESBEN PETER FRIIS, AKIHIKO YAMAGISHI