Abstract: A method of processing a sample for analysis is disclosed. The method includes providing a first sample-transfer article with a sample disposed therein, a container (1110), a pierceable cap (1120), and a pierceable layer (1500). The method further includes operably coupling the cap (1120) and the container (1110), disposing the pierceable layer (1500) adjacent the cap (1120), urging the first sample-transfer article through the layer (1500) and the cap (1120), transferring a portion of the sample into the container (1110), and separating the layer (1500) from the cap (1120). The method further can comprise providing a second sample transfer article having a second sample delivery end and urging the second sample-transfer article through the cap. A kit comprising a container, a cap, a pierceable layer; and means for coupling the pierceable layer to the cap and/or to the container is also provided.