Abstract: A polypeptide selected from the group of lysosomal enzymes and lysosomal enzyme activators, comprising at least one introduced glycosylation site as compared to a corresponding parent enzyme or activator. By introducing additional glycosylation sites the resulting glycosylated lysosomal enzyme or activator obtains improved in vivo activity and thereby provides for improved treatment of lysosomal storage diseases.

Abstract: The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.

Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.

Abstract: The present invention relates to a 35 kDa enzyme exhibiting aminopeptidase activity which is derived from a fungal microorganism, a DNA construct comprising a DNA sequence encoding said enzyme, a recombinant expression vector comprising said DNA construct, and a cell comprising said DNA sequence. The present invention also provides a method for producing said enzyme exhibiting aminopeptidase activity, and an enzyme preparation comprising said enzyme, a bread-improving or dough-improving composition comprising the aminopeptidase of the invention. Finally the invention relates to the use of said enzyme exhibiting aminopeptidase activity or enzyme preparations or compositions thereof.

Abstract: Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.

Abstract: The present invention relates to a DNA construct exhibiting &bgr;-1,3-glucanase activity, which DNA sequence comprises: a) the DNA sequence shown in SEQ ID No. 1 or SEQ ID No. 12, or b) an analogue of the DNA sequence defined in a), which i) is homologous with the DNA sequence defined in a), or ii) hybridizes with the same oligonucleotide probe as the DNA sequence defined in a), or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence defined in a), or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified &bgr;-1,3-glucanase derived from Oerksovia xanthineolytica LLG109 encoded by the DNA sequence defined in a). The DNA construct may further encode a mannose binding domain. The invention also relates to a DNA construct encoding a mannose binding domain.

Abstract: The present invention relates to polypeptides having laccase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides.

Abstract: The present invention relates to polypeptides having laccase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides.

Abstract: The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.

Abstract: An isolated polypeptide having &agr;-galactosidase activity and characterized as having a pH optimum in the range of 5.0-7.0, and a temperature optimum within the range of 50-70° C. The &agr;-galactosidase is derived from Aspergillus niger.

Abstract: A method for identifying a transglutaminase-producing microorganism based on a selective assay is disclosed.

Abstract: An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.

Abstract: The present invention relates to a cloned DNA sequence encoding an enzyme with dextranase activity, a recombinant expression vector comprising said DNA sequence, a filamentous fungus host cell, a method for producing said recombinant dextranase, and the isolated and purified enzyme.The invention also relates to compositions comprising the recombinant enzyme, oral care compositions and products and the use for removing of dental plaque.

Abstract: The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorus content, wherein said method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of said enzyme for a number of industrial applications.

Abstract: The present invention relates to an enzyme exhibiting aminopeptidase activity, a method for producing said enzyme, an enzyme preparation containing said enzyme exhibiting aminopeptidase activity, and use of said enzyme for various industrial purposes.

Abstract: An acidic phospholipase is obtained from a strain of the genus Hyphozyma. It is able to hydrolyze both fatty acyl groups in intact phospholipid. Advantageously, it has no lipase activity and is active at very low pH; these properties make it very suitable for use in oil degumming, as enzymatic and alkaline hydrolysis (saponification) of the oil can both be suppressed. The phospholipase is not membrane bound, making it suitable for commercial production and purification.

Abstract: This invention is a protease mutant wherein said mutant substitutes the naturally occurring tyrosine amino acids for other amino acids at positions 91, 167, 171, 192, 209, 214, and 263. The protease mutants are used in detergent compositions.

Abstract: The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorous content, wherein the method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of enzyme for a number of industrial applications.

Abstract: Transglutaminase preparations are producible by a wide range of fungi, especially ascomycotina, basidiomycotina and zygomycota, and gram-negative and gram-positive bacteria, especially Streptomyces lydicus, NRRL B-3446. A DNA construct encoding a novel transglutaminase and comprising the DNA sequence obtainable from the plasmid in E. coli, DSM 10175, is also described together with a method of producing the transglutaminases, a composition comprising the transglutaminase and a method for producing a gel or protein gelation composition.