Abstract: The present invention provides reagents and methods for RNA transfection and protein expression.

Abstract: Disclosed is a method of amplifying and detecting cytokeratin 19 mRNA in RNA amplification process, comprising: a step for forming a double-stranded DNA containing a promoter sequence with a reverse transcriptase by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous to a portion of cytokeratin 19 mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5?-end of either the first primer or the second primer, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, by measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.

Abstract: Disclosed is a method of amplifying and detecting mRNA of survivin gene in an RNA amplification process comprising: a step for forming a double-stranded DNA containing a promoter sequence by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous with a portion of survivin mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5?-end of either the first primer or the second primer with a reverse transcriptase, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.

Abstract: The present invention provides reagents and methods for RNA transfection and protein expression

Abstract: The amount of an RNA transcription product amplified in an RNA amplification process is measured using a nucleic acid probe labeled with an intercalating fluorescent dye. The RNA amplification process comprises the steps of using at least two sets of primer pairs comprising a first primer and a second primer (in which one of these primers carries a promoter sequence added to the 5? end thereof), both of which have high hybridization efficiency to a nucleic acid sequence that is homologous to or complementary to each norovirus genotype RNA; forming a double-stranded DNA containing the promoter sequence with a reverse transcriptase; forming an RNA transcription product with an RNA polymerase by using the double-stranded DNA as a template; and forming the double-stranded DNA by successively using the RNA transcription product as a template in the DNA synthesis with the reverse transcriptase.

Abstract: To detect a bacterium belonging to the genus Legionella rapidly, with high sensitivity, and specifically. Disclosed are: a primer set for amplifying ribosomal RNA of a bacterium belonging to the genus Legionella specifically and highly efficiently; and a method for detecting ribosomal RNA of a bacterium belonging to the genus Legionella by using the primer set.

Abstract: A seat reclining mechanism comprises a guide bracket, and an internal gear member coupled to the guide bracket in a rotatable manner about a rotation axis thereof with respect to the guide bracket. A forwardmost-inclination stopper protrusion and a rearwardmost-inclination stopper protrusion are provided on either one of the guide bracket and the internal gear member. A stopper plate is fixed to a remaining one of the guide bracket and the internal gear member. The stopper plate has a forwardmost-inclination stopper portion adapted to be brought into contact with the forwardmost-inclination stopper protrusion when a seat back is at a forwardmost inclined position, and a rearwardmost-inclination stopper portion adapted to be brought into contact with the rearwardmost-inclination stopper protrusion when the seat back is at a rearwardmost inclined position.

Abstract: A method for assaying ?1,4-N-acetylgiucosamine transferase (?4GnT) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5?-end of a specified nucleotide sequence of the RNA and a second primer complementary to at least a portion upstream from the 3?-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5?-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.

Abstract: Disclosed is a seat reclining mechanism, which is designed to be mounted to a seat cushion and a seat back so as to allow the seat back to be reclined. The seat reclining mechanism comprises a guide bracket and an internal gear member coupled to the guide bracket in a rotatable manner about a rotation axis thereof with respect to the guide bracket and formed with internal teeth along a circle having a center on the rotation axis. A plurality of lock gear members is disposed between the guide bracket and the internal gear member. A fitting portion is provided in either one of the guide bracket and the internal gear member. A rotatable cam is rotatably supported by the fitting portion.

Abstract: A method of extracting biological material-derived nucleic acid from samples containing biological material, the method comprising mixing biological material with an aqueous solution containing at least a surfactant, subjecting the mixture to heating treatment and then removing the aqueous solution to separate the biological material, adding a solid powder suspension to the biological material, agitating or sonicating it to disrupt the biological material, and extracting nucleic acid from the biological material.

Abstract: A seat reclining mechanism comprises a guide bracket, and an internal gear member coupled to the guide bracket in a rotatable manner about a rotation axis thereof with respect to the guide bracket. A forwardmost-inclination stopper protrusion and a rearwardmost-inclination stopper protrusion are provided on either one of the guide bracket and the internal gear member. A stopper plate is fixed to a remaining one of the guide bracket and the internal gear member. The stopper plate has a forwardmost-inclination stopper portion adapted to be brought into contact with the forwardmost-inclination stopper protrusion when a seat back is at a forwardmost inclined position, and a rearwardmost-inclination stopper portion adapted to be brought into contact with the rearwardmost-inclination stopper protrusion when the seat back is at a rearwardmost inclined position.

Abstract: Disclosed is a seat reclining mechanism, which is designed to be mounted to a seat cushion and a seat back so as to allow the seat back to be reclined. The seat reclining mechanism comprises a guide bracket and an internal gear member coupled to the guide bracket in a rotatable manner about a rotation axis thereof with respect to the guide bracket and formed with internal teeth along a circle having a center on the rotation axis. A plurality of lock gear members is disposed between the guide bracket and the internal gear member. A fitting portion is provided in either one of the guide bracket and the internal gear member. A rotatable cam is rotatably supported by the fitting portion.

Abstract: A seat reclining mechanism comprises a guide bracket, an internal gear member coupled to the guide bracket in a rotatable manner about a rotation axis thereof with respect to the guide bracket, a plurality of lock gear members disposed between the guide bracket and the internal gear member, and a rotatable cam rotatably supported by either one of the guide bracket and the internal gear member. The rotatable cam has a cam body, and a plurality of engagement pawls disposed on a circumference of a circle having a center on the rotation axis and each formed to protrude from the cam body in a lock-causing rotation direction or an unlock-causing rotation direction, and each of the plurality of lock gear members has an engagement groove engageable with a corresponding one of the plurality of engagement pawls.

Abstract: In an RNA amplification process comprising steps of using a first primer and a second primer, at least one of which has a promoter sequence at the 5? end, and reverse transcriptase, to produce double-stranded DNA containing the promoter sequence, using the double-stranded DNA as template to produce an RNA transcript with RNA polymerase, and using the RNA transcript in turn as template for DNA synthesis with the reverse transcriptase to produce the double-stranded DNA, the amount of amplified RNA product is measured with an intercalating fluorescent dye-labeled nucleic acid probe.

Abstract: A seat reclining mechanism comprises a plurality of lock gear members. One of the plurality of lock gear members has a protrusion which protrudes toward an internal gear member. The internal gear member has a first free-zone arc portion formed along a circle having a center on a rotation axis and adapted to allow the protrusion to be in contact therewith when the lock gear members are located at a position where external teeth of each of lock gear members are not in meshing engagement with internal teeth of the internal gear, and a second free-zone arc portion formed along the circle to have an arc length different from that of the first free-zone arc portion and adapted to allow the protrusion to be in contact therewith when the lock gear members are located at the position.

Abstract: A method of detecting micrometastasis of tumor cells in a sample obtained from a region of a subject other than the primary focus of the tumor by using a first primer complementary to part of a specific sequence in the RNA from tumor cells and a second primer homologous to part of the specific sequence (either of which additionally has a promoter sequence for an RNA polymerase at the 5? end), which comprises (1) synthesizing a cDNA by the action of an enzyme having RNA-dependent DNA polymerase activity by using the specific sequence as a template, (2) degrading the RNA strand in the RNA-DNA double strand by an enzyme having a ribonuclease H activity (to give a single-stranded DNA), (3) forming a double-stranded DNA having a promoter sequence which can be transcribed into an RNA homologous or complementary to the specific sequence by using the single-stranded DNA as a template by the action of an enzyme having DNA-dependent DNA polymerase activity, and (4) transcribing the double-stranded DNA into an RNA trans

Abstract: A method for assaying heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5?-end of a specified nucleotlde sequence of the RNA and a second primer complementary to at least a portion upstream from the 3?-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5?-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.

Abstract: In an RNA amplification process comprising steps of using a first primer and a second primer, at least one of which has a promoter sequence at the 5? end, and reverse transcriptase, to produce double-stranded DNA containing the promoter sequence, using the double-stranded DNA as template to produce an RNA transcript with RNA polymerase, and using the RNA transcript in turn as template for DNA synthesis with the reverse transcriptase to produce the double-stranded DNA, the amount of amplified RNA product is measured with an intercalating fluorescent dye-labeled nucleic acid probe.

Abstract: A device includes a first substrate having a channel formed on front and rear surfaces of the first substrate, and an information recording layer forming an optical recording medium on at least one of the front and rear surfaces of the first substrate.

Abstract: The present invention provides an RNA amplification process, comprising steps in which double-stranded DNA containing a promoter sequence is produced using a first primer and second primer, at least one of which has the promoter sequence at the 5? end, and reverse transcriptase, the double-stranded DNA is used as template for production of RNA transcript using RNA polymerase and the RNA transcript is used as template for subsequent DNA synthesis with the reverse transcriptase to produce the double-stranded DNA. The process yields an amplified RNA product which is assayed with a fluorescent intercalator dye-labeled nucleic acid probe.