Abstract: Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.

Abstract: Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.

Abstract: The invention relates to a method for the non-invasive selection of single living cells under gentle conditions from mixtures of cells or cell cultures with respect to a specific production performance. To this end, the concentration of substances produced by the individual cells which become enriched at the cell membrane, such as reporter gene products (GFP) or specifically secreted products, such as antibodies, is determined by fluorescence-microscopic detection methods.

Abstract: The invention relates to methods and devises for the faithful modeling of organ functions in vitro.

Abstract: The invention relates to ubiquitous/universal processes for establishing cells capable of stable high yield expression of a recombinant gene with human glycosylation pattern, and for establishing stable universal precursor cells available for insertion of arbitrary target genes.

Abstract: Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.

Abstract: The invention relates to a method for the non-invasive selection of single living cells under gentle conditions from mixtures of cells or cell cultures with respect to a specific production performance. To this end, the concentration of substances produced by the individual cells which become enriched at the cell membrane, such as reporter gene products (GFP) or specifically secreted products, such as antibodies, is determined by fluorescence-microscopic detection methods.

Abstract: The invention relates to culture systems and methods for continuous sterile cultivation of cells in high densities and for reducing the inoculation density at the beginning of cell cultivation in bioreactors. By using biodegradable gels that release low-molecular-weight constituents as nutrients for the cell culture or semi-solid media that are diluted during the course of the culturing and thus free up culture space for colonization with cells in high density, it is possible to sharply reduce the starting cell density at the beginning of cultivation in bioreactors. Biodegradable gels used are polypeptide (block) copolymers, in part comprising poly-L-glutamine, and semi-solid media used are methylcellulose, alginates, and agaroses.

Abstract: A polymer mixture of a thermoplastically processable fluoropolymer and a sulfur polymer, wherein the sulfur polymer component in the polymer mixture is 0.1 to 20% by weight.

Abstract: The invention relates to a method and a device which allow to cultivate cells in a sterile way over long time periods and at high densities and to obtain the maximum product volumes for time units from the cell cultures. Here the cultivation chamber and the supply container are semipermeably separated from each other, wherein the cultivation chamber comprises several fixed chambers and wherein the supply chamber is connected to a device for generating of a variably adjustable gas/cell cultivation medium mixture.

Abstract: The invention relates to an electrical line (L) having at least one electrical conductor (1) enclosed by temperature-resistant insulation (2) which ensures the functionality of the line (1) in case of fire. To minimize the fire load of the line (1), the insulation (2) comprises at least one multifilament thread (3) made of glass which is wound around the conductor (1) and whose windings are contiguous so as to create a completely closed sleeve (4) for the conductor (1). A thin protective layer (5) of a halogen-free, temperature-resistant insulation material is applied all over the sleeve (4).

Abstract: The invention relates to an electrical line (L) having at least one electrical conductor (1) enclosed by temperature-resistant insulation (2) which ensures the functionality of the line (1) in case of fire. To minimize the fire load of the line (1), the insulation (2) comprises at least one multifilament thread (3) made of glass which is wound around the conductor (1) and whose windings are contiguous so as to create a completely closed sleeve (4) for the conductor (1). A thin protective layer (5) of a halogen-free, temperature-resistant insulation material is applied all over the sleeve (4).

Abstract: A polymer mixture of a thermoplastically processable fluoropolymer and a sulfur polymer, wherein the sulfur polymer component in the polymer mixture is 0.1 to 20% by weight.

Abstract: A process and device are disclosed for simultaneously cultivating different mammal cells, for separately obtaining different mammal cell products and for simulating organic interactions on the humoral plane. Essentially, the invention consists of arranging several culture vessels in a common supply circuit and or cultivating different mammal cells in separate vessels.

Abstract: A process and device are disclosed for simultaneously cultivating different mammal cells, for separately obtaining different mammal cell products and for simulating organic interactions on the humoral plane. Essentially, the invention consists of arranging several culture vessels in a common supply circuit and of cultivating different mammal cells in separate vessels.