Patents by Inventor Victor Lyamichev

Victor Lyamichev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11054425
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.
    Type: Grant
    Filed: February 7, 2020
    Date of Patent: July 6, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Mohamed Yosry Hassan Mohamed, Tobias Oelschlaegel, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Patent number: 10976317
    Abstract: The invention comprises systems, methods and arrays for identification and optimization of novel peptide binders to protein targets. Embodiments include steps of peptide binder discovery, core peptide maturation, N-terminal and C-terminal extension and kinetics analysis of the final peptide binder.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: April 13, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Jigar Patel, Victor Lyamichev, Thomas Albert
  • Patent number: 10947272
    Abstract: The present invention relates to dual-site BACE1 inhibitors, their manufacture, pharmaceutical compositions containing them and their use as therapeutically active substances. The active compounds of the present invention are useful in the therapeutic and/or prophylactic treatment of e.g. Alzheimer's disease.
    Type: Grant
    Filed: July 21, 2016
    Date of Patent: March 16, 2021
    Assignees: Hoffmann-La Roche Inc., ROCHE NIMBLEGEN, INC.
    Inventors: Jeremy Beauchamp, Per-Ola Freskgard, Eric A. Kitas, Victor Lyamichev, Jigar Patel
  • Publication number: 20200371102
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.
    Type: Application
    Filed: August 12, 2020
    Publication date: November 26, 2020
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Mohamed Yosry Hassan Mohamed, Tobias Oelschlaegel, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Patent number: 10788495
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: September 29, 2020
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Publication number: 20200249231
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.
    Type: Application
    Filed: February 7, 2020
    Publication date: August 6, 2020
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Mohamed Yosry Hassan Mohamed, Tobias Oelschlaegel, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Patent number: 10393751
    Abstract: The invention provides novel peptide binders for streptavidin (SA), Taq polymerase and several human proteins: Prostate Specific Antigen (PSA), thrombin, Tumor Necrosis Factor Alpha (TNF?), and Urokinase-type Plasminogen Activator (uPA).
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: August 27, 2019
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventors: Thomas Albert, Ryan Bannen, Victor Lyamichev, Jigar Patel, Eric Sullivan
  • Publication number: 20190086411
    Abstract: The invention comprises systems, methods and arrays for identification and optimization of novel peptide binders to protein targets. Embodiments include steps of peptide binder discovery, core peptide maturation, N-terminal and C-terminal extension and kinetics analysis of the final peptide binder.
    Type: Application
    Filed: November 14, 2018
    Publication date: March 21, 2019
    Inventors: Jigar Patel, Victor Lyamichev, Thomas Albert
  • Patent number: 10161938
    Abstract: The invention comprises systems, methods and arrays for identification and optimization of novel peptide binders to protein targets. Embodiments include steps of peptide binder discovery, core peptide maturation, N-terminal and C-terminal extension and kinetics analysis of the final peptide binder.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: December 25, 2018
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Jigar Patel, Victor Lyamichev, Thomas Albert
  • Publication number: 20180356424
    Abstract: The disclosure provides compositions comprising at least one assembly comprising a peptide and a major histocompatibility complex (MHC), wherein the peptide is an integral component of the MHC, wherein the peptide is attached to a surface at its C-terminus through a linker and wherein the peptide is synthesized on the surface. In certain embodiments, the compositions comprise a plurality of assemblies in a spatially-ordered array. The disclosure provides methods for making and using these compositions.
    Type: Application
    Filed: September 5, 2017
    Publication date: December 13, 2018
    Inventors: Christian Klein, Hanying Li, Victor Lyamichev, Ekkehard Moessner, Jigar Patel
  • Publication number: 20180252714
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.
    Type: Application
    Filed: May 8, 2018
    Publication date: September 6, 2018
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Publication number: 20180179252
    Abstract: The present invention relates to dual-site BACE1 inhibitors, their manufacture, pharmaceutical compositions containing them and their use as therapeutically active substances. The active compounds of the present invention are useful in the therapeutic and/or prophylactic treatment of e.g. Alzheimer's disease.
    Type: Application
    Filed: July 21, 2016
    Publication date: June 28, 2018
    Inventors: Jeremy BEAUCHAMP, Per-Ola FRESKGARD, Eric A. KITAS, Victor LYAMICHEV, Jigar PATEL
  • Patent number: 10006915
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: June 26, 2018
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Publication number: 20170314054
    Abstract: According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.
    Type: Application
    Filed: June 16, 2017
    Publication date: November 2, 2017
    Inventors: THOMAS ALBERT, FRANK BERGMANN, VICTOR LYAMICHEV, JIGAR PATEL, MICHAEL SCHRAEML, WOJTEK STEFFEN
  • Publication number: 20170152543
    Abstract: The present disclosure provides a method of identifying a substrate for a protease including contacting a protease to one of a first array and a second array, each array having the same plurality of features, each feature including at least one sequence linked to a solid support. The at least one sequence includes a candidate protease substrate linked to a reporter. The method further includes contacting a detectable element to each of the arrays to allow binding of the detectable element to the reporter, and detecting first and second signals resulting from binding of the detectable element to the each of the reporters in the first and second arrays. The method further includes comparing the first signal and the second signal to identify a difference in the first signal and the second signal, and identifying at least one candidate protease substrate as a substrate for the protease.
    Type: Application
    Filed: November 29, 2016
    Publication date: June 1, 2017
    Inventors: Lauren Goodrich, Christian Klein, Victor Lyamichev, Jigar Patel, Eric Sullivan
  • Publication number: 20160367961
    Abstract: This invention relates to peptide microarrays, methods of generating peptide microarrays, and methods of identifying peptide binders using microarrays. More specifically, this invention relates to peptide microarrays, methods of generating peptide microarrays, and methods of identifying peptide binders using microarrays wherein the microarrays comprise cyclic peptides. The invention also relates to methods of increasing the number of cyclized peptides on a microarray by treating the peptides on the microarray with a protease. Additionally, the invention relates to methods of generating linear and cyclic peptides subarrays on a microarray.
    Type: Application
    Filed: April 22, 2016
    Publication date: December 22, 2016
    Inventors: Jigar Patel, Lauren Goodrich, Victor Lyamichev, Thomas Albert
  • Publication number: 20160312212
    Abstract: This invention relates to a method of selecting a peptide using an mRNA-displayed cow antibody scaffold polypeptide. The invention also relates to the mRNA-displayed cow antibody scaffold polypeptide comprising a peptide of interest or to a peptide microarray comprising the cow antibody scaffold polypeptide comprising a peptide of interest or to a peptide microarray comprising a peptide of interest as described herein.
    Type: Application
    Filed: April 22, 2016
    Publication date: October 27, 2016
    Inventors: Jigar Patel, Hanying Li, Victor Lyamichev, Thomas Albert, Lauren Goodrich
  • Publication number: 20160305952
    Abstract: The invention provides novel peptide binders for streptavidin (SA), Taq polymerase and several human proteins: Prostate Specific Antigen (PSA), thrombin, Tumor Necrosis Factor Alpha (TNF?), and Urokinase-type Plasminogen Activator (uPA).
    Type: Application
    Filed: April 19, 2016
    Publication date: October 20, 2016
    Inventors: Thomas Albert, Ryan Bannen, Victor Lyamichev, Jigar Patel, Eric Sullivan
  • Publication number: 20160178627
    Abstract: In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases.
    Type: Application
    Filed: December 17, 2015
    Publication date: June 23, 2016
    Inventors: Thomas Albert, Frank Bergmann, Victor Lyamichev, Jigar Patel, Michael Schraeml, Wojtek Steffen, Thomas Streidl
  • Patent number: 9133503
    Abstract: The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: September 15, 2015
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Allawi, Victor Lyamichev