Abstract: An example method includes capturing a plurality of images of a rotating object using an imaging device optically coupled to a microscope. The method removing a first portion of a model of the rotating object based on a first contour of the rotating object in a first image of the plurality of images. The method includes orienting the model based on an amount of rotation of the rotating object between capture of the first image and capture of a second image of the plurality of images. The method also includes removing a second portion of the model of the rotating object based on a second contour of the rotating object in the second image.

Abstract: A surface enhanced luminescence (SEL) sensor may include a substrate and nano fingers extending from the substrate. In one implementation, the nano fingers may be arranged in a cluster of at least three nano fingers extending from the substrate. The nano fingers of the cluster having different geometries so as to bend into a closed state such that each of the nano fingers of the cluster are linked to one another.

Abstract: A system and a method for the batch sorting of particles are provided. An example of a batch sorting system includes a microfluidic ejector, a flow channel fluidically coupled to the microfluidic ejector at one end, and a reservoir coupled to an opposite end of the flow channel from the microfluidic ejector. A counter is disposed in the flow channel upstream of the microfluidic ejector to count particles prior to ejection from the microfluidic ejector. An optical sensor is to image the flow channel. A controller is configured to locate a target particle in the flow channel based, at least in part, on the image and capture the target particle in a collection vessel based, at least in part, on a count from the counter.

Abstract: A microfluidic device may, in an example, include at least one microfluidic channel, a dielectrophoresis separator to separate a plurality of cells passing within the at least one microfluidic channel, and a thermal resistor to eject at least one cell from the microfluidic device. A cassette may, in an example, include a die coupled to a substrate of the cassette, the die including at least one microfluidic channel, a dielectrophoresis separator along the microfluidic channel to separate a plurality of cells passing within the microfluidic channel, and an ejection device to eject at least one of the plurality of cells into an assay well.

Abstract: An apparatus includes a polymer base layer having a surface. A die has a surface that is substantially coplanar with the surface of the polymer base layer. The die includes a fluidic actuator to control fluid flow across the surface of the die. A fluidic channel is coupled to the polymer base layer to provide a fluidic interconnect between the die and a fluidic input/output port.

Abstract: In example implementations, an apparatus is provided. The apparatus includes a channel, an energy source, and a transfection chamber. The channel includes an indentation to hold a cell. The energy source is to apply a shockwave to the cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.

Abstract: In example implementations, an apparatus is provided. The apparatus includes a channel, a thermal inkjet (TIJ) resistor, and a transfection chamber. The TIJ resistor is to apply heat to a cell in the channel to porate the cell. The transfection chamber is to store a reagent to be inserted into the cell after the cell is porated.

Abstract: The present disclosure is drawn to a method of labeling and identifying microfluidic samples. The method can include tagging a first microfluidic sample with a first combination of markers to obtain a first labeled sample; tagging a second microfluidic sample with a second combination of markers that is different than the first combination of markers to obtain a second labeled sample; introducing a common variable to the first labeled sample and the second labeled sample. The common variable can generates a first interaction with the first labeled sample that is different than a second interaction or lack of interaction with the second labeled sample. The method can further include based on the first interaction, identifying the first labeled sample by assaying for the first combination of markers.

Abstract: Examples disclosed herein relate to single cell transfection with interchangeable reagent. The present disclosure relates generally to a device, method, and system for single cell transfection including a transfection chamber on a transfection chip. The example system may include a fluidic channel located on the transfection chip for guiding a cell towards the transfection chamber, the fluidic channel sized to allow no more than a single cell to arrive at the transfection chamber at a time. The example system may also include a reagent receiver located on the transfection chip guiding received reagent towards the transfection chamber and intersecting with the path of the fluidic channel.

Abstract: In example implementations, a cell trapping array is provided. The cell trapping array includes a plurality of plates coupled along adjacent edges to form a channel. A plurality of orifices are formed in a first plate of the plurality of plates of the channel. The plurality of orifices is shaped to create a meniscus of a fluid in the channel in the plurality of orifices that is to attract a single cell from cells flowing through the channel in the fluid. The cell trapping array includes a selective ejection system coupled to a second plate located opposite the first plate of the channel. The selective ejection system is to selectively eject the single cell from one of the plurality of orifices.

Abstract: A fluid entrained particle separator may include an inlet passage to direct particles entrained in a fluid, a first separation passage branching from the inlet passage, a second separation passage branching from the inlet passage and electrodes to create electric field exerting a dielectrophoretic force on the particles to direct the particles to the first separation passage or the second separation passage, wherein the first separation passage, the second separation passage, the electric field and the dielectrophoretic force extend in a plane.

Abstract: An example system includes a microfluidic cavity; a retaining feature within the microfluidic cavity, and a releasing feature. The retaining feature is to position capsules at a predetermined location in the microfluidic cavity. The capsules have a thermally degradable shell enclosing a material therein. The releasing feature is to selectively cause degradation of the shell to release the material into the microfluidic opening. The releasing feature is to generate heat to facilitate degradation of the shell. In some examples, the retaining feature is a physical barrier sized to prevent flow of the capsule and to allow flow of the released materials through the microfluidic cavity.

Abstract: A surface enhanced luminescence (SELS) sensor may include a substrate and nano fingers projecting from the substrate. Each of the nano fingers may include a polymer pillar having a sidewall and a top, a coating layer covering the sidewall and a metal cap supported by and in contact with the top of the pillar.

Abstract: The present disclosure relates to a microfluidic device including a microfluidic substrate and dry reagent-containing particles. The microfluidic substrate includes an ingress microfluidic channel that fluidly feeds an egress microfluidic channel through a microfluidic-retaining region that includes a microfluidic discontinuity feature, a particle-retaining chemical coating, or a combination thereof. The dry reagent-containing particles include a reagent that is releasable from the dry reagent-containing particles when exposed to a release fluid. The dry reagent-containing particles are retained within the microfluidic substrate at the microfluidic discontinuity feature or particle-retaining chemical coating in position to release the reagent into the egress microfluidic channel upon flow of release fluid from the ingress microfluidic channel through the microfluidic-retaining region.

Abstract: A microfluidic device is provided that includes a substrate and microfluidic sub-chips embedded in the substrate. An electric field is applied between an adjacent pair microfluidic sub-chips to move a fluid droplet from one of the adjacent pair of microfluidic sub-chips to another of the adjacent pair microfluidic sub-chips.

Abstract: A device may include a substrate, a first fluid processing chip, a second fluid processing chip, a tapered channel, and a fluid actuator. The first fluid processing chip may be disposed on the substrate and may process a micro-volume of fluid. The second fluid processing chip may be disposed on the substrate and co-planar with the first fluid processing chip. The second fluid processing chip may process at least a portion of the micro-volume of fluid. The tapered channel may be disposed between the first and second fluid processing chips to transport the at least the portion of the micro-volume of fluid from the first fluid processing chip to the second fluid processing chip. The fluid actuator may be disposed proximate to the tapered channel and may control movement of the at least the portion of the micro-volume of fluid within the tapered channel.

Abstract: The present disclosure relates to a microfluidic particle concentrator that includes an inlet microchannel, a filtering chamber fluidly connected to the inlet microchannel to receive a sample fluid, and a mechanical filter positioned in the filtering chamber. The particle concentrator also includes a filter outlet microchannel fluidly connected to the filtering chamber to receive a particle-ablated fluid formed by passing through the mechanical filter, a particle outlet microchannel fluidly connected to the filtering chamber to receive a particle-concentrated fluid including a plurality of particles not permitted to pass through the mechanical filter, and a fluid movement network including multiple pumps. The multiple fluid pumps generate sample fluid flow through the inlet microchannel and into the filtering chamber, particle-ablated fluid flow from the mechanical filter into the filter outlet microchannel, and particle-concentrated fluid from the filtering chamber into the particle outlet microchannel.

Abstract: A system includes a microchannel analysis region, a first fluid actuation device, a second fluid actuation device, a sensor, and a controller. The first fluid actuation device is at a first end of the microchannel analysis region. The second fluid actuation device is at a second end of the microchannel analysis region opposite to the first end. The sensor is within the microchannel analysis region between the first fluid actuation device and the second fluid actuation device. The sensor measures an impedance of a fluid within the microchannel analysis region. The controller activates the first fluid actuation device to generate a first pressure wave in the fluid and activates the second fluid actuation device to generate a second pressure wave in the fluid. The first pressure wave and the second pressure wave converge at the sensor.

Abstract: Blood being circulated to mimic the environment it naturally inhabits increases growth of cultures including bacteria for analysis. The present disclosure presents examples of a device, method, computer-readable medium, and other techniques for simulating a natural environment for blood. The techniques may include a channel with a hydrophobic interior surface and a fluid mover to encourage continuous fluid flow through the channel. The techniques may further include a gas exchange opening of the channel that exposes a fluid to gas outside of the channel.

Abstract: A particle imaging system may include a volume to contain a fluid having a suspended particle, electrodes proximate to the volume to apply an electric field to rotate the suspended particle, an optical sensor comprising a first region and a second region and a diffraction element to split an image of the suspended particle into a bright field image focused on the first region and a spectral image focused on the second region.