Abstract: The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules.

Abstract: The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules.

Abstract: Molecular determinants of cancer response to immunotherapy are described, as are systems and tools for identifying and/or characterizing cancers likely to respond to immunotherapy. The present invention encompasses the discovery that the likelihood of a favorable response to cancer immunotherapy can be predicted. The present invention further comprises the discovery that cancer cells may harbor somatic mutations that result in neoepitopes that are recognizable by a patient's immune system as non-self. The identification of one or more neoepitopes in a cancer sample is useful for determining which cancer patients are likely to respond favorably to immunotherapy, in particular, treatment with an immune checkpoint modulator.

Abstract: The invention relates to polynucleotide combinations and their use in allele-specific enrichment, amplification, and detection. The disclosure also provides methods to multiplex various target DNA molecules in a single tube with high sensitivity and specificity. The disclosure provides a polynucleotide competitor that comprises a sequence that is fully complementary to a first target DNA polynucleotide region (T1) such that the competitor polynucleotide will hybridize to the first target DNA polynucleotide region under appropriate conditions. In another aspect, the polynucleotide competitor comprises a mismatch to a non-target DNA polynucleotide that is a sequence variant of the first target DNA polynucleotide region (T1*).

Abstract: The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3? end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing.

Abstract: A polynucleotide device is provided that adds one or more bases to a single stranded polynucleotide. Methods of using the device are provided, comprising contacting a single stranded target molecule with an extension reaction mixture comprising (i) a device or composition of the disclosure, (ii) a polymerase, and (iii) free nucleotides, whereupon an extension reaction product is generated. Kits comprising a device of the disclosure are also provided.

Abstract: Methods of isolating target double-stranded polynucleotides with internal single-stranded regions are provided. Compositions and kits comprising double-stranded polynucleotides with internal single-stranded regions are also provided.

Abstract: The present disclosure provides a novel technology that involves improved primer design. These primer pairs have a wide range of applications and provide high sensitivity and specificity.

Abstract: The present invention provides a novel technology that involves improved primer design. These primer pairs have a wide range of applications and provide high sensitivity and specificity.

Abstract: The present invention provides a novel technology that involves improved primer design. These primer pairs have a wide range of applications and provide high sensitivity and specificity.

Abstract: According to certain general aspects, the present invention relates to a method and apparatus for allowing commands for an application to be effectively and conveniently executed. In general embodiments, an application executed on a tablet computer automatically senses fingertips on the tablet and forms command regions around them. Commands are associated with the command regions, and when a touch event occurs in a command region (e.g. a finger tap), the information is sent to a client application possibly running on a remote host, where the associated command is executed.

Abstract: Diamond thin films were deposited on copper substrate by the Vapor Solid (VS) deposition method using a mixture of fullerene C60 and graphite as the source material. The deposition took place only when the substrate was kept in a narrow temperature range of approximately 550-650° C. Temperatures below and above this range results in the deposition of fullerenes and other carbon compounds, respectively.

Abstract: A mechanism for performing register allocation based on priority spills and assignments is disclosed. A method of embodiments of the invention includes repetitively detecting fat points during a compilation process of a software program running on a virtual machine of a computer system, each fat point representing a program point having a high register pressure, the high register pressure occurs when a number of live program variables of the software program living at a given program point of the software program is greater than a number of available processor registers of the computer system. The method further includes choosing a fat point with a highest register pressure, selecting a live program variable having a lowest priority at the chosen fat point, and spilling the lowest priority live program variable to memory of the computer system.

Abstract: A mechanism for performing instruction scheduling based on register pressure sensitivity is disclosed. A method of embodiments of the invention includes performing a preliminary register pressure minimization on program points during a compilation process of a software program running on a virtual machine of a computer system. The method further includes calculating a register pressure at each of the program points, detecting an instruction to be scheduled, and performing instruction scheduling of the instruction based on a current register pressure at a current scheduling point and potential register pressures at subsequent scheduling points.

Abstract: The invention presents a simple, non-destructive and non-abrasive method of diamond nucleation using polyethene. It particularly describes the nucleation of diamond on an electrically viable substrate surface using polyethene via chemical vapor deposition (CVD) technique in a gaseous environment.

Abstract: Methods of isolating target double-stranded polynucleotides with internal single-stranded regions are provided.

Abstract: Materials and methods are provided for the gel-free fractionation of polynucleotide molecules. According to the present invention, fractionation is size-based or sequence-based.

Abstract: A mechanism for performing instruction scheduling based on register pressure sensitivity is disclosed. A method of embodiments of the invention includes performing a preliminary register pressure minimization on program points during a compilation process of a software program running on a virtual machine of a computer system. The method further includes calculating a register pressure at each of the program points, detecting an instruction to be scheduled, and performing instruction scheduling of the instruction based on a current register pressure at a current scheduling point and potential register pressures at subsequent scheduling points.

Abstract: A mechanism for performing register allocation based on priority spills and assignments is disclosed. A method of embodiments of the invention includes repetitively detecting fat points during a compilation process of a software program running on a virtual machine of a computer system, each fat point representing a program point having a high register pressure, the high register pressure occurs when a number of live program variables of the software program living at a given program point of the software program is greater than a number of available processor registers of the computer system. The method further includes choosing a fat point with a highest register pressure, selecting a live program variable having a lowest priority at the chosen fat point, and spilling the lowest priority live program variable to memory of the computer system.

Abstract: A polynucleotide device is provided that add one or more bases to a single stranded polynucleotide. Methods of using the device and kits comprising the device are also provide.