Abstract: An acousto-optical component is suggested, in particular for use in the beam path of an optical arrangement, preferably of a microscope, with a crystal for the passage of light. Sound waves are generated via an electrical transducer and passed through the crystal. The sound waves passing through the crystal affect the optical properties of the crystal and therefore the light passing through the crystal. For exerting a variable influence on the light passing through the crystal, the sound waves passing through the crystal, or the acoustic field in the crystal, are/is variable.

Abstract: A photosensor chip includes a light-sensitive region with a plurality of detector elements and a light-insensitive region including a buffer memory. The light-insensitive region is at least double the light-sensitive region. A charge produced at different points in time in the detector elements is successively transferred into the buffer memory for being subsequently read out by a read-out register from the buffer memory. In one embodiment, the buffer memory includes a first and second buffer memory adjoining corresponding sides of the light-sensitive region. The first and second buffer memory can be used alternatively for buffer-storage and read-out.

Abstract: An apparatus, a microscope having an apparatus, and a method for calibration of a photosensor chip (19) are disclosed. The apparatus has a photosensor chip (19) which has a multiplicity of light-sensitive elements. A reference light source (30) is provided and directs the light at at least one part of the photosensor chip (19). In addition, an open-loop or closed-loop control unit (19a) is provided and determines and corrects variances between the individual light-sensitive elements.

Abstract: An optical arrangement having an optical assembly (1) for generating an intermediate image (2) in a beam path is configured and further developed such that an optical element (3) having a plurality of optical waveguides (4) is arranged in the beam bath after the intermediate image (2) to enable the imaging of the intermediate image (2) in a plane on a zone-by-zone or point-by-point basis. Further, a microscope exhibiting such an optical arrangement is specified.

Abstract: A method for investigating transport processes in a preferably biological specimen, a laser light beam (2, 3) being guided by means of a scanning apparatus line by line over the specimen within definable specimen regions, and the light proceeding from the specimen being detected by means of a detection apparatus, is characterized, in the interest of the capability of investigating, with high accuracy, processes within the specimen that proceed on a short time scale, in that both an image production light beam (5) for the purpose of observing the specimen and a manipulation light beam (4) for the purpose of manipulating the specimen are used as the laser light beam (2, 3), the image production light beam (5) preceding the manipulation light beam (4) in such a way that pixels of the specimen not yet manipulated by the manipulation light beam (4) are illuminated with the image production light beam (5).

Abstract: The method and the system simplify moving interactions by means of virtual reference subjects and flux-based coordinate transformations in order to generate a changeable frame of reference.

Abstract: A device for beam adjustment in an optical beam path, having at least two mutually independent light sources (1, 2), in particular in a beam path (8, 9) of a preferably high or extremely high resolution microscope, the beams of the light sources (1, 2) requiring to be superposed in a common illumination beam path (10), is characterized in that a calibration sample (22) with the aid of which the pupil position and/or focal position of the beams can be checked can be brought into and taken out of the illumination beam path (10).

Abstract: The invention relates to a photosensor-chip, a laser-microscope comprising a photosensor-chip and to a method for reading a photosensor-chip. Said photosensor-chip comprises a light-sensitive area and a light-insensitive area. The light-insensitive area has a surface which is at least the double the surface of the light-sensitive area.

Abstract: The invention relates to a confocal microscope which illuminates a sample (15) by means of at least one light source. A detection light beam (17) is emitted from the sample (15). The detection light beam (17) is spectrally split up in a spatial manner by the dispersive element (20) and subsequently formed on a photosensor chip (19) by means of a detection optical system (22). At least one expanding optical system (23) is arranged in front of the dispersive element (20) in the direction of the detection light beam (17). The expanding optical system (23) is embodied in such a manner that the numerical aperture of the detection optical system (22) is independent from the numerical aperture of the detection light beam (17) on the detection apertured diaphragm (18).

Abstract: An EMCCD detector includes a first gain register and a photoactive area divided into at least a two detection regions. At least one of the detection regions is assigned to the first gain register.

Abstract: A detector includes a photosensitive array including at least one photosensitive surface. A focusing device focuses spectrally split light onto the photosensitive array. The focusing device is located in an optical path upstream from the photosensitive array. The focusing device includes a microlens array including at least one microlens.

Abstract: A device for generating a laser light beam includes a module. The module includes at least one laser light source, and a mechanical, an electrical and/or an optical interface defined towards an outside of the module.

Abstract: A device for generating a light beam having several wavelengths includes a beam recombiner arrangement for recombining several laser light beams having different wavelengths. The beam recombiner arrangement includes several individual beam recombiners arranged in a row or in groups parallel to each other and each configured to couple in a respective laser light beam having a wavelength of a defined wavelength range.

Abstract: A position measuring device for detecting the spatial position of a movable element in relation to a base body, the device including a linear measuring device that measures a distance between a movable element and a base body and an angle-measuring apparatus that measures an angle between the movable element and the base body. A light source that directs light along a beam path, a detector within the beam path and a grating within the beam path between the light source and the detector. For measuring the angle, the beam path extends between the movable element and the base body so that an intensity strip pattern is created by illuminating the grating by the light source, whose position relative to the detector is a measure of the angle.

Abstract: A method for high spatial resolution examination of samples, preferably by using a laser scanning fluorescence microscope, the sample (1) to be examined comprising a substance that can be repeatedly converted from a first state (Z1, A) into a second state (Z2, B), the first and the second states (Z1, A; Z2, B) differing from one another in at least one optical property, comprising the following steps: a) the substance is brought into the first state (Z1, A) by means of a switching signal (2) in a sample region (P) to be recorded, b) the second state (Z2, B) is induced by means of an optical signal (4), spatially delimited subregions being specifically excluded within the sample region (P) to be recorded, c) the remaining first states (Z1, A1, A2, A3) are read out by means of a test signal (7), and d) steps a) to c) are repeated, the optical signal (4) being displaced upon each repetition in order to scan the sample (1), is defined in that the individual steps a) to d) are carried out in a sequence adapted

Abstract: An apparatus, a microscope having an apparatus, and a method for calibration of a photosensor chip (19) are disclosed. The apparatus has a photosensor chip (19) which has a multiplicity of light-sensitive elements. A reference light source (30) is provided and directs the light at at least one part of the photosensor chip (19). In addition, an open-loop or closed-loop control unit (19a) is provided and determines and corrects variances between the individual light-sensitive elements.

Abstract: An apparatus for detecting photons of a light beam emanating from a spatially limited source includes a detection device having a plurality of detectors forming a three-dimensional array with semitranslucent EMCCDs disposed one behind another. A light splitting device is disposed in a path of rays of the light beam for splitting the light beam so as to distribute the photons over the detectors for detection.

Abstract: A device for selectively detecting specific wavelength components of a light beam includes a spectral spreading element for spectrally spreading the light beam, and a detector array arranged downstream of the element. The detector array includes light-insensitive regions and light-sensitive regions. The element and the detector array are matched to each other so that selectable wavelength components of the light beam hit the light-insensitive regions and remaining wavelength components of the light beam hit the light-sensitive regions.

Abstract: The invention relates to an optical device containing a dispersive element and a projection lens, which define a splitting plane on which a location is assigned to each wavelength. A microstructures element is located on the splitting plane, the element guiding light beams, which emanate from different directions and are focused on the locations corresponding to their wavelengths, through the projection lens to the dispersive element, which combines the light beams in a collinear manner.

Abstract: A detection device includes a spectral splitting device located in a detection beam path for spectrally splitting detection light into individual spectral components. A deflection device is located downstream of the spectral splitting device for deflecting the individual spectral components in different deflection directions onto detectors assigned to the individual spectral components. At least one optical element is located in the detection beam path downstream of the spectral splitting device and upstream of the deflection device such that at least one of the individual spectral components incident on the deflection device is collimated.