Abstract: In a method for scanning microscopy an illuminating light beam that contains at least first light of a first wavelength and second light of a second wavelength, is coded. The coded illuminating light beam is directed onto a specimen and detection light proceeding from the specimen is decoded.

Abstract: An apparatus for detecting photons of a light beam emanating from a spatially limited source includes a detection device having a plurality of detectors forming a three-dimensional array with semitranslucent EMCCDs disposed one behind another. A light splitting device is disposed in a path of rays of the light beam for splitting the light beam so as to distribute the photons over the detectors for detection.

Abstract: A detector includes a CCD arrangement having at least one CCD, and a focusing device. The focusing device focuses spectrally separated light onto the CCD arrangement. The focusing device is located in an optical path before the CCD arrangement, and includes a microlens arrangement having at least one microlens.

Abstract: A detector includes a photosensitive array including at least one photosensitive surface. A focusing device focuses spectrally split light onto the photosensitive array. The focusing device is located in an optical path upstream from the photosensitive array. The focusing device includes a microlens array including at least one microlens.

Abstract: A method for microscopy includes generating pulsed illuminating light including wavelengths in a spectral region. A detection spectral region within the spectral region is defined. Using a dynamically controllable mask, light components of the illuminating light that comprise wavelengths within the detection spectral region are influenced. A specimen is illuminated with the influenced illuminating light. Detection light proceeding from the specimen within the detection spectral region is detected.

Abstract: A method for separating different emission wavelengths in a scanning microscope includes scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector, and selectively applying each of a plurality of excitation wavelengths to the illuminating light beam during the scanning according to a predefinable illumination scheme. Emission light coming from the specimen is detected using a detector, the emission light including emission wavelengths corresponding to the excitation wavelengths. The detector is read out when an excitation wavelength is applied so as to provide detected signals. The detected signals are associated with the respective excitation wavelengths using the illumination scheme.

Abstract: An EMCCD detector includes a first gain register and a photoactive area divided into at least a two detection regions. At least one of the detection regions is assigned to the first gain register.

Abstract: A method for superimposing optical information in a scanning microscope includes determining a transformation matrix, and superimposing first optical information of a CCD image and second optical information of at least one piece of second image information using the transformation matrix.

Abstract: A method for investigating transport processes in a preferably biological specimen, a laser light beam (2, 3) being guided by means of a scanning apparatus line by line over the specimen within definable specimen regions, and the light proceeding from the specimen being detected by means of a detection apparatus, is characterized, in the interest of the capability of investigating, with high accuracy, processes within the specimen that proceed on a short time scale, in that both an image production light beam (5) for the purpose of observing the specimen and a manipulation light beam (4) for the purpose of manipulating the specimen are used as the laser light beam (2, 3), the image production light beam (5) preceding the manipulation light beam (4) in such a way that pixels of the specimen not yet manipulated by the manipulation light beam (4) are illuminated with the image production light beam (5).

Abstract: The invention discloses a method for microscopy in which a specimen is illuminated with pulsed illuminating light that comprises light from a spectral region, and detection light proceeding from the specimen is detected in a detection spectral region. The method is characterized in that the detection spectral region lies within the spectral region, and that the illuminating light contains no light from the detection spectral region or at least none having the same polarization properties. A microscope is additionally disclosed.

Abstract: An angle measuring system for the contactless determination of an angle between a first object and a second object which can be moved with respect to each other, the angle measuring system including a first component having a light source and connected to a first object and a second component having a photodetector and connected to a second object. A grating is connected either to the first component or the second component, the grating having a combined amplitude-phase grating.

Abstract: A microscope includes at least one illuminating light source and an illumination beam path for guiding the illuminating light to a specimen. A detection beam path guides detected light from the specimen to a detector. An activatable element is positioned in the detection beam path for regulating and/or limiting the light power level in the detection beam path.

Abstract: A detection device includes a spectral splitting device located in a detection beam path for spectrally splitting detection light into individual spectral components. A deflection device is located downstream of the spectral splitting device for deflecting the individual spectral components in different deflection directions onto detectors assigned to the individual spectral components. At least one optical element is located in the detection beam path downstream of the spectral splitting device and upstream of the deflection device such that at least one of the individual spectral components incident on the deflection device is collimated.

Abstract: A device for generating a light beam having several wavelengths includes a beam recombiner arrangement for recombining several laser light beams having different wavelengths. The beam recombiner arrangement includes several individual beam recombiners arranged in a row or in groups parallel to each other and each configured to couple in a respective laser light beam having a wavelength of a defined wavelength range.

Abstract: A device for generating a laser light beam includes a module. The module includes at least one laser light source, and a mechanical, an electrical and/or an optical interface defined towards an outside of the module.

Abstract: A method for separating different emission wavelengths in a scanning microscope includes scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector, and selectively applying each of a plurality of excitation wavelengths to the illuminating light beam during the scanning according to a predefinable illumination scheme. Emission light coming from the specimen is detected using a detector, the emission light including emission wavelengths corresponding to the excitation wavelengths. The detector is read out when an excitation wavelength is applied so as to provide detected signals. The detected signals are associated with the respective excitation wavelengths using the illumination scheme.

Abstract: An optical arrangement for directing illumination light to a sample and for directing the detection light proceeding from the sample to a detector has a spectrally selective influencing means in the beam path of the detection light, which influences the polarization properties of the illumination light reflected or scattered from the sample in such a way that a polarizing beam splitter splits the illumination light reflected or scattered from the sample out of the detection light.

Abstract: A device for selectively detecting specific wavelength components of a light beam includes a spectral spreading element for spectrally spreading the light beam, and a detector array arranged downstream of the element. The detector array includes light-insensitive regions and light-sensitive regions. The element and the detector array are matched to each other so that selectable wavelength components of the light beam hit the light-insensitive regions and remaining wavelength components of the light beam hit the light-sensitive regions.

Abstract: A position measuring device for detecting the spatial position of a movable element in relation to a base body, the device including a linear measuring device that measures a distance between a movable element and a base body and an angle-measuring apparatus that measures an angle between the movable element and the base body. A light source that directs light along a beam path, a detector within the beam path and a grating within the beam path between the light source and the detector. For measuring the angle, the beam path extends between the movable element and the base body so that an intensity strip pattern is created by illuminating the grating by the light source, whose position relative to the detector is a measure of the angle.

Abstract: A detection device, in particular for use in a laser scanning microscope, includes a means (2) located in a detection beam path (1) to spectrally split detection light into individual spectral components (3, 4), and further includes a deflection device (5) located downstream of the means (2) for spectral splitting to deflect the individual spectral components (3, 4) in different deflection directions onto detectors (6) assigned to the individual spectral components (3, 4). With a view to reliable separation of the individual spectral components (3, 4) deflected by deflection device (5), the detection device is built and further refined in such a way that at least one optical element (7) is arranged in the detection beam path (1) downstream of the means (2) for spectral splitting and upstream of the deflection device such that at least one spectral component (3, 4) of the light incident on the deflection device (5) is collimated in at least one spatial direction.