Patents by Inventor Wayne P. Fitzmaurice
Wayne P. Fitzmaurice has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20110111413Abstract: The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.Type: ApplicationFiled: November 23, 2010Publication date: May 12, 2011Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
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Patent number: 7838219Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: August 8, 2003Date of Patent: November 23, 2010Assignee: Novici Biotech LLCInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
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Patent number: 7833759Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: June 25, 2007Date of Patent: November 16, 2010Assignee: Novici Biotech LLCInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Patent number: 7582423Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.Type: GrantFiled: October 25, 2002Date of Patent: September 1, 2009Assignee: Novici Biotech LLCInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Patent number: 7235386Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: July 25, 2002Date of Patent: June 26, 2007Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Patent number: 7217514Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: GrantFiled: July 25, 2002Date of Patent: May 15, 2007Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Patent number: 7132588Abstract: The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability tostabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.Type: GrantFiled: July 21, 2003Date of Patent: November 7, 2006Assignee: Large Scale Biology CorporationInventors: Wayne P. Fitzmaurice, Gregory P. Pogue, John A. Lindbo
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Patent number: 7056740Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.Type: GrantFiled: January 31, 2003Date of Patent: June 6, 2006Assignee: Large Scale Biology CorporationInventors: Hal S. Padgett, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith, John A. Lindbo, Wayne P. Fitzmaurice
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Publication number: 20040180352Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: August 8, 2003Publication date: September 16, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
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Publication number: 20040142433Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.Type: ApplicationFiled: October 10, 2003Publication date: July 22, 2004Inventors: Hal S. Padgett, Wayne P. Fitzmaurice, John A. Lindbo, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith
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Publication number: 20040110130Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.Type: ApplicationFiled: October 25, 2002Publication date: June 10, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Publication number: 20040106198Abstract: The invention provides a system for expressing a foreign peptide in a plant cell, wherein the foreign protein is sensitive to a protease activity in the plant cell, by introducing into a plant cell a polynucleotide, which encodes the foreign protein and an another polynucleotide, which encodes a genetic element capable of reducing the protease activity in the plant cell. The invention also provides for plant cells, which incorporate this system, and for methods of reducing the proteolysis of the foreign protein expressed in a plant cell by using this system.Type: ApplicationFiled: July 16, 2003Publication date: June 3, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventors: Kathleen M. Hanley, Fakhrieh S. Vojdani, Long V. Nguyen, Wayne P. Fitzmaurice
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Publication number: 20040060087Abstract: The present invention relates to novel interspecific Nicotiana excelsior×N. benthamiana hybrid seeds and plants and to a method of producing interspecific Nicotiana hybrids having enhanced properties for biomass and the production of recombinant proteins using a viral vector system.Type: ApplicationFiled: September 18, 2003Publication date: March 25, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventor: Wayne P. Fitzmaurice
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Publication number: 20040060086Abstract: The present invention relates to novel interspecific Nicotiana excelsior×N. benthamiana hybrid seeds and plants and to a method of producing interspecific Nicotiana hybrids having enhanced properties for biomass and the production of recombinant proteins using a viral vector system.Type: ApplicationFiled: September 18, 2003Publication date: March 25, 2004Applicant: LARGE SCALE BIOLOGY CORPORATIONInventor: Wayne P. Fitzmaurice
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Patent number: 6656726Abstract: The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability to stabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.Type: GrantFiled: May 4, 2000Date of Patent: December 2, 2003Assignee: Large Scale Biology CorporationInventors: Wayne P. Fitzmaurice, Gregory P. Pogue, John A. Lindbo
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Publication number: 20030186261Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: July 25, 2002Publication date: October 2, 2003Applicant: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
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Publication number: 20030157682Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.Type: ApplicationFiled: January 31, 2003Publication date: August 21, 2003Inventors: Hal S. Padgett, Andrew A. Vaewhongs, Fakhrieh S. Vojdani, Mark L. Smith, John A. Lindbo, Wayne P. Fitzmaurice
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Publication number: 20030036641Abstract: The invention provides methods of forcing recombination between polynucleotides. The methods can include the steps of, (a) generating a single strand of a first polynucleotide; (b) generating a single strand of a second polynucleotide, wherein the second polynucleotide is partially complementary to the first polynucleotide; (c) fragmenting the single strand of the first polynucleotide to generate single stranded first polynucleotide fragments; (d) fragmenting the single strand of the second polynucleotide to generate single stranded second polynucleotide fragments; (e) annealing the single stranded first polynucleotide fragments with the single stranded second polynucleotide fragments; and (f) extending the annealed polynucleotide fragments.Type: ApplicationFiled: January 31, 2001Publication date: February 20, 2003Inventors: Hal S. Padgett, Wayne P. Fitzmaurice, John A. Lindbo
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Publication number: 20030027173Abstract: The present invention provides methods for rapidly determining the function of nucleic acid sequences by transfecting the same into a host organism to effect expression. Phenotypic and biochemical changes produced thereby are then analyzed to ascertain the function of the nucleic acids which have been transfected into the host organism. The invention also provides methods for silencing endogenous genes by transfecting hosts with nucleic acid sequences to effect expression of the same. The present invention also provides methods for selecting desired functions of RNAs and proteins by the use of virus vectors to express libraries of nucleic acid sequence variants. Moreover, the present invention provides methods for inhibiting an endogenous protease of a plant host.Type: ApplicationFiled: February 5, 2002Publication date: February 6, 2003Inventors: Guy Della-Cioppa, Robert L. Erwin, Wayne P. Fitzmaurice, Kathleen Hanley, Monto H. Kumagai, John A. Lindbo, David R. McGee, Hal S. Padgett, Gregory P. Pogue
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Publication number: 20020177160Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.Type: ApplicationFiled: July 25, 2002Publication date: November 28, 2002Applicant: Large Scale Biology CorporationInventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice