Abstract: The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.

Abstract: Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-21-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described.

Abstract: The present invention relates to methods of detecting anionic proteins in a sample with fluorescent carbocyanine dye compounds. The invention also describes methods of simultaneously detecting anionic and non-anionic proteins in a sample with discrete fluorescent signals produced by carbocyanine dye compounds. The invention is of use in a variety of fields including immunology, diagnostics, molecular biology and fluorescence based assays.

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.

Abstract: The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers.

Abstract: A video decoder transcodes video data from various input formats to a predetermined output format. Input data may be standard definition data or MPEG2 compressed data. Standard definition data are rearranged into block format to be compatible with the decoder's single display processor. The display processor selectively processes and conveys either MPEG2 format data or non-MPEG2 format data to a display device. A block based frame memory stores MPEG2 and non-MPEG2 pixel block data, as well as standard definition data in raster line format during processing.

Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

Abstract: Apparatus for displaying a combined image of an auxiliary image and a main image includes a source of a main image signal and a source of samples representing an auxiliary image signal. A quincunx subsampler is coupled to the auxiliary image sample source and the quincunx subsampler. The sample combiner combines the main image signal and a signal representing the quincunx subsampled samples to generate a signal representing a combined image of the main and auxiliary images.

Abstract: Apparatus for combining an auxiliary image and a main image includes a source of a main image video signal and a source of samples representing an auxiliary image video signal having successive fields. A quincunx subsampler is coupled to the auxiliary image sample source and subsamples the auxiliary image samples in either a first sample pattern in which samples are taken at a first set of horizontal locations, or a second sample pattern in which samples are taken at a second set of horizontal locations substantially midway between the first set, all in response to a control signal. A signal combiner is coupled to the quincunx subsampler and the main image signal source, and combines the main image signal and a signal representing the quincunx subsampled auxiliary image samples to generate a signal representing a combined image of the main and auxiliary images.

Abstract: Apparatus for combining an auxiliary image and a main image includes a source of a main image signal and a source of samples representing an auxiliary image signal. A subsampler selectively subsamples the auxiliary image samples in either a quincunx subsampling pattern or a rectangular subsampling pattern. A signal combiner combines the main image signal and a signal representing the subsampled auxiliary image samples to generate a combined image signal. A control circuit generates a freeze control signal for the subsampler which conditions it to take samples in the rectangular subsampling pattern during a freeze frame operation, and in the quincunx sampling pattern otherwise.

Abstract: In order to selectively reduce the size of an auxiliary image, such as may be displayed within main image during a so called "picture-in-picture" or "PIP" mode of operation of a television system, a fixed amount of the auxiliary image is caused to be cropped from the auxiliary image without changing the compression ratio of the compressed auxiliary video signal from which the auxiliary image is derived.

Abstract: A method for monitoring a condition, such as the presence of electrical power, at a remote location and for generating a telephone call on to a telephone line that delivers to a central control unit notification of a change in the monitored condition in the form of a set of unique identifying signals. According to the method, the condition is monitored at the remote location and is sensed for changes in the condition from a first state to a second state. Responsive to a change in the condition to the second state, the telephone line is tested and a line availability signal generated. Responsive to indicia of line availability, the line is selectively seized.

Abstract: A method and apparatus for providing information, such as power consumption, to a subscriber over an electronic network in response to commands provided by the subscriber. The apparatus includes a remote unit which detects and stores the power consumption information, a subscriber device, i.e., a telephone, for generating request commands and a control device which tracks the information. The subscriber using the subscriber device, feeds the request commands via the network to the control device. The control device responds to the commands by directing the remote unit to feed its stored power consumption information onto the network to the control device. The control device then processes the information and indicates the result back to the subscriber.