Abstract: Herein disclosed is a microfluidic device comprising: semi-spiral-shaped channels in fluid communication with (i) at least two inlet ports and (ii) at least two outlet ports, wherein the at least two inlet ports comprise: a sample inlet port and a sheath inlet port, wherein the sample inlet port is in fluid communication with sample inlet channels, each of the sample inlet channels is connected to one semi-spiral-shaped channel, and wherein the sheath inlet port is in fluid communication with sheath inlet channels, each of the sheath inlet channels is connected to one semi-spiral-shaped channel; wherein the at least two outlet ports comprise a first outlet port and each of the semi-spiral-shaped channels has a first outlet channel connected to the first outlet port, and wherein each first outlet channel is longer than any other outlet channel connected to the same semi-spiral-shaped channel. A method of fractionating particles is also disclosed.

Abstract: An optical connector includes a fiber positioning assembly and a frame having two walls for supporting the fiber positioning assembly in a controlled vertical alignment relative to the frame. The fiber positioning assembly includes an optical fiber array disposed at a top surface of a support block for positioning between the walls, and a cap covering top portions of the optical fibers projecting from the block. The cap has a flat alignment surface resting upon the fiber top portions and extending laterally beyond the support block such that when the block is disposed between the walls, free portions of the alignment surface rest upon the walls to control the vertical alignment of the array. A single rail affixed to a bottom shelf of the frame, and a correspondingly dimensioned rectangular notch at the bottom of the support block, control the horizontal alignment of the array.

Abstract: An optical connector includes a fiber positioning assembly and a frame having two walls for supporting the fiber positioning assembly in a controlled vertical alignment relative to the frame. The fiber positioning assembly includes an optical fiber array disposed at a top surface of a support block for positioning between the walls, and a cap covering top portions of the optical fibers projecting from the block. The cap has a flat alignment surface resting upon the fiber top portions and extending laterally beyond the support block such that when the block is disposed between the walls, free portions of the alignment surface rest upon the walls to control the vertical alignment of the array. A single rail affixed to a bottom shelf of the frame, and a correspondingly dimensioned rectangular notch at the bottom of the support block, control the horizontal alignment of the array.

Abstract: A water exchange base station including a main body, a sewage exchange port, a clear water exchange port, a first sewage connecting port, a first clear water connecting port arranged on the main body, and an inlet valve; when the clear water port of the intelligent cleaning machine is docked with the clear water exchange port and in the state of supplying clear water, the clear water exchange port supplies the clear water to the intelligent cleaning machine; when the sewage port of the intelligent cleaning machine is docked with the sewage exchange port and in the state of sewage discharging, the sewage inside the intelligent cleaning machine is drawn out from the sewage exchange port and discharged from the first sewage connecting port to the outside.

Abstract: A blockchain system, and related devices, systems, and methods are provided. A blockchain node device node device receives a first message from a gaming device in communication with the blockchain node device, the first message including a request for first play data corresponding to historical wagering activity by a player and wagering limit data associated with a limitation on wagering activity by the player. The blockchain node transmits a first blockchain block of a plurality of blockchain blocks of a blockchain data structure to the gaming device, wherein the first blockchain block includes the first play data. The blockchain node receives a second message from the gaming device, the second message including a second blockchain block including hash data associated with the first blockchain block and second play data corresponding to further wagering activity by the player. The blockchain node adds the second blockchain block to the blockchain data structure.

Abstract: An optics-free method for single cell profiling, particularly white blood cells such as neutrophils is disclosed. Embodiments relate to multi-parametric biophysical profiling of neutrophils using a microfluidic impedance-deformability cytometry device. The device comprises a first flow channel comprising a particle focusing region, a detection region comprising a first pair of electrodes, a cell deformation zone and a second pair of electrodes. A multi-frequency impedance response of each cell before and after deformation is measured respectively by the first and second pairs of electrodes, such as to quantify membrane opacity, nucleus opacity, cell size and deformability index. A second flow channel bifurcated into two split flow pathways that converge in an intersecting manner with the first flow channel forms a cross-junction in a cell deformation zone, to hydrodynamically deform each cell by perfusing a sheath fluid along the two split flow paths from two opposite sides of the sample flow channel.

Abstract: Herein disclosed is a method of encapsulating a spheroid in a gel, the method comprising: providing a frame comprising a base and an island protruding from the base; depositing one or more suspensions on the island, wherein the one or more suspensions comprise different cells; arranging the frame to have the one or more suspensions hang from the island in a direction which gravity acts to render growth of the spheroid; depositing a gel on the spheroid with the spheroid resting on the island to have the gel encapsulate the spheroid; and arranging the frame against a substrate with the base distally positioned from the substrate (i) to have the gel confined between the island and the substrate and (ii) to have the gel encapsulate the spheroid.

Abstract: An opening and closing device for curtains and a system based on the same, mounted on a curtain track, comprises a main engine and a roller mechanism, The main engine comprises a shell with a cavity, a driving wheel mounted in the cavity and partially exposed the shell, a motor arranged in the cavity and controlling the driving wheel to rotate, a controller lied in the cavity and controlling the motor to start and stop, and a signal transceiver module coupled electrically with the controller and receiving starting and stopping signals of the motor; the driving wheel is used for driving the curtains to move along the curtain track; a roller mechanism is mounted on the shell, an end, far away from the shell, of the roller mechanism is hung on the curtain track and presses the driving wheel closely on the curtain track.

Abstract: The present invention relates to a microfluidic impedance cytometer comprising an integrated biosensor and use of the device thereof for label-free activation profiling of cells in a sample, such as myelocytes and/or lymphocytes and neutrophils. The microfluidic impedance cytometer may comprise a spiral-shaped flow channel, coplanar electrodes for generating an electric field across the channel, a first sample inlet and a second sheath fluid inlet; wherein the sample inlet is a stepped sample inlet in that the height of the sample inlet is lower than the height of the sheath fluid inlet and the flow channel.

Abstract: An opening and closing device for a curtain mounted on a guiding part, the guiding part is provided with the curtain; the opening and closing device is used for driving automatically the opening and closing of the curtain; the opening and closing device includes a mainframe and a connecting mechanism. The mainframe includes a shell, a driver, and a driving wheel; the driving wheel and the driver are mounted in the shell, the driver drives the driving wheel to rotate. The connecting mechanism includes a hanging piece and a bracket; a first end of the bracket is inserted into the shell, the hanging piece is movably connected with a second end of the bracket and hung on the guiding part; the hanging piece can be moved vertically relative to the bracket so that the driving wheel is attached closely to the outer wall of the guiding part.

Abstract: A shutter controller configured to control an operating rod of a shutter includes a controller housing, a clamping mechanism, and a driving mechanism. The controller housing includes a first housing and a second housing connected to each other, and the second housing is provided with a receiving cavity. The clamping mechanism is configured to clamp the operating rod and rotatably provided in the receiving cavity. The driving mechanism is provided in the first housing and is in transmission connection with the clamping mechanism, and the driving mechanism is configured to drive the clamping mechanism to rotate, thereby driving the operating rod to rotate.

Abstract: Provided are an SGLT-2 inhibitor-sarcosine cocrystal, a preparation method therefor and use thereof. Using sarcosine as a ligand, said cocrystal has higher safety and lower costs; a drug cocrystal has higher stability, and during the production process or storage process of a formulation composition, the crystal form is not easily changed; said cocrystal does not melt when heated, does not stick, aggregate or generate static electricity, and has better mixing uniformity. Said cocrystal has uniform distribution in a prepared pharmaceutical composition, causing that the pharmaceutical composition has better in-vivo release, absorption and efficacy, and has small difference between batches; and the pharmaceutical composition has high stability, and is more conducive to storage and transportation.

Abstract: A method of monitoring one or more cell aggregates, comprising providing a flow path in which the one or more cell aggregates are in a medium and the flow path being configured to pass through a collective sensing zone of a set of electrodes, obtaining impedance-related signals corresponding to each of the medium and one of the one or more cell aggregates in the medium, determining one or more electrical signatures for a cell aggregate, in which the one or more electrical signatures are based on impedance-related signals obtained from the set of electrodes. The method is one of dynamic testing at single-particle resolution. The electrical signatures may be an opacity and/or electrical size of the one or more cell aggregates, or electrical impedance spectroscopy-based electrical signatures. The cell aggregate is a spheroid, an encapsulated microcarrier, or a cell-adhered microcarrier. It is also to provide a microfluidic chip comprising a channel and electrodes for obtaining impedance- related signals.

Abstract: The present disclosure provides a device for patterning extracellular matrix (ECM) hydrogel comprising a first layer surface patterned to define a microchannel, a second layer comprising a loading channel in fluid communication with loading ports to receive an ECM hydrogel, wherein the first layer is attached over the second layer such that the patterned surface faces the loading channel to define an open chamber with regions of reduced cross-sectional area, and wherein the ECM hydrogel is confined to fill said regions, thereby forming a perfusable channel in the open chamber. The present disclosure also provides the same device wherein the second layer is a substrate without a loading channel and is optically pervious; and additionally provides a method of patterning ECM hydrogel comprising use of the aforementioned device. Importantly, ECM patterning is achieved by surface tension between the ECM hydrogel and the first layer at the boundaries of the microchannel.

Abstract: A server chassis includes a housing and two support portions. A bottom plate of the housing includes a loading surface and a three-dimensional reinforcing pattern integrally formed on the loading surface for reinforcing the structural strength of the bottom plate. The support portions are respectively located on an outer surface of the sidewall of the housing. A coverage area of the three-dimensional reinforcing pattern is greater than 10% of the total area of the loading surface. The three-dimensional reinforcing pattern includes a plurality of texture patterns regularly reproduced on the loading surface toward a linear axial direction. The three-dimensional reinforcing pattern also includes a plurality of first arc portions and a plurality of second arc portions arranged in a staggered alignment.

Abstract: A method herein to isolate exosomes includes providing a microfluidic device having a spiral-shaped channel in fluid communication with two inlet ports and at least two outlet ports. One of the two inlet ports is proximal to an inner wall of the spiral-shaped channel and the other is proximal to an outer wall thereof. At least one of the outlet ports is in fluid communication with a container for storing isolated exosomes. A blood sample and sheath fluid are introduced into the inlet ports proximal to the outer and inner walls, respectively, to form a diluted sample in the spiral-shaped channel and driven through for exosomes recovery in the container. The spiral-shaped channel in fluid communication with a first outlet port includes a first outlet channel connecting the spiral-shaped channel to the first outlet port and is longer than other outlet channels respectively connecting the spiral-shaped channel to the other outlet ports. A method of identifying diabetes mellitus is also disclosed herein.

Abstract: The present invention relates to high-strength high-toughness low-temperature thick-plate structural steel and a heat treatment method thereof. The steel is composed of the following components by weight percentage: C: 0.03-0.08%, Cr: 0.8-1.9%, Mn: 0.01-1.0%, Ni: 3.5-7%, Mo: 0.2-0.5%, V: 0.15-0.2%, Nb: 0.01-0.05%, Cu: 1.2-3.8%, Al: 0-0.5%, P: <0.015%, S: <0.010%, and Fe and inevitable impurities as balance. Compared with the prior art, a steel plate prepared in the present invention can be used at low temperature of ?20 to ?120° C. and ?196° C., maintains relatively high strength and certain toughness, and mainly resolves a technical problem that existing high-strength high-toughness quenched and tempered steel cannot meet equipment requirements in polar resource and energy development; transportation, etc.

Abstract: An opening and closing device for curtains and a system based on the same, mounted on a curtain track, comprises a main engine and a roller mechanism, The main engine comprises a shell with a cavity, a driving wheel mounted in the cavity and partially exposed the shell, a motor arranged in the cavity and controlling the driving wheel to rotate, a controller lied in the cavity and controlling the motor to start and stop, and a signal transceiver module coupled electrically with the controller and receiving starting and stopping signals of the motor; the driving wheel is used for driving the curtains to move along the curtain track; a roller mechanism is mounted on the shell, an end, far away from the shell, of the roller mechanism is hung on the curtain track and presses the driving wheel closely on the curtain track.

Abstract: An opening and closing device for a curtain mounted on a guiding part, the guiding part is provided with the curtain; the opening and closing device is used for driving automatic opening and closing of the curtain; the opening and closing device includes a mainframe and a connecting mechanism. The mainframe includes a shell, a driver and a driving wheel; the driving wheel and the driver are mounted in the shell, the driver drive the driving wheel to rotate. The connecting mechanism includes a hanging piece and a bracket; a first end of the bracket is inserted into the shell, the hanging piece is movably connected with a second end of the bracket and hung on the guiding part; the hanging piece can be moved vertically relative to the bracket so that the driving wheel is attached closely to the outer wall of the guiding part.

Abstract: A transparent conductive film is disclosed. The transparent conductive film includes a substrate and a first silver nanowire layer. The transparent conductive film has a first absorption peak at 340 nm to 400 nm and a second absorption peak at 500 nm-650 nm, and a ratio of a maximum peak intensity of the first absorption peak to a maximum peak intensity of the second absorption peak is in a range of 2 to 5.5.