Abstract: A pre-saturated disposable disinfecting wipe may include a microfiber cloth. The microfiber cloth may include a low weight substrate having a first face and an opposed second face, and microfiber yarn attached to the low weight substrate in a plurality of parallel rows of stitches. The pre-saturated disposable disinfecting wipe further includes a disinfecting formulation saturated in the microfiber cloth. The pre-saturated disposable disinfecting wipe may have a single-touch two-minute wetness coverage of more than 100 in2. A method of manufacturing a pre-saturated disposable disinfecting wipe may include saturating, with a disinfecting formulation, a microfiber cloth.

Abstract: This disclosure relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3?-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This disclosure further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.

Abstract: This disclosure relates to an enzymatic method of synthesizing a polynucleotide, comprising a deprotecting step which uses as a deprotecting agent a specific phosphonate compound. It also pertains to a method for deprotecting 3?-O-amino elongated fragments of a polynucleotide in an enzymatic method of synthesizing a polynucleotide, comprising contacting the elongated fragments with this phosphonate compound. This disclosure further relates to a kit for synthesizing a polynucleotide comprising one or more vials of synthesis reagents, at least one of which contains an effective amount of a phosphonate compound and to a specific method for preparing said phosphonate compound.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: The present application is directed to, in part, nucleotide analogs with a novel design of a 3?-OH reversible terminator that comprises an enzyme-cleavable linkage.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: A pre-saturated disposable disinfecting wipe may include a microfiber cloth. The microfiber cloth may include a low weight substrate having a first face and an opposed second face, and microfiber yarn attached to the low weight substrate in a plurality of parallel rows of stitches. The pre-saturated disposable disinfecting wipe further includes a disinfecting formulation saturated in the microfiber cloth. The pre-saturated disposable disinfecting wipe may have a single-touch two-minute wetness coverage of more than 100 in2. A method of manufacturing a pre-saturated disposable disinfecting wipe may include saturating, with a disinfecting formulation, a microfiber cloth.

Abstract: A pre-saturated disposable disinfecting wipe may include a microfiber cloth. The microfiber cloth may include a low weight substrate having a first face and an opposed second face, and microfiber yarn attached to the low weight substrate in a plurality of parallel rows of stitches. The pre-saturated disposable disinfecting wipe further includes a disinfecting formulation saturated in the microfiber cloth. The pre-saturated disposable disinfecting wipe may have a single-touch two-minute wetness coverage of more than 100 in2. A method of manufacturing a pre-saturated disposable disinfecting wipe may include saturating, with a disinfecting formulation, a microfiber cloth.

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Methods and compositions are described for multi-stage primer extension reactions such as multiplex polymerase chain reactions (PCR) and reverse transcriptase PCR. Primer extension stages are performed in a closed vessel without opening the vessel between stages. The multi-stage primer extension methods and compositions utilize earlier stage primers in an earlier stage and later stage primers in a later stage, wherein the later stage primers are blocked from extension during the earlier stage. The blocked primers of the present technology comprise photocleavable blocking groups and are substantially inactive until the blocking group is cleaved by exposure to ultraviolet light. The blocked primers can be activated by ultraviolet light without opening the vessel.

Abstract: A pre-saturated disposable disinfecting wipe may include a microfiber cloth. The microfiber cloth may include a low weight substrate having a first face and an opposed second face, and microfiber yarn attached to the low weight substrate in a plurality of parallel rows of stitches. The pre-saturated disposable disinfecting wipe further includes a disinfecting formulation saturated in the microfiber cloth. The pre-saturated disposable disinfecting wipe may have a single-touch two-minute wetness coverage of more than 100 in2. A method of manufacturing a pre-saturated disposable disinfecting wipe may include saturating, with a disinfecting formulation, a microfiber cloth.

Abstract: An absorbent article is provided. The absorbent article includes a nonwoven, liquid permeable topsheet, a liquid impermeable backsheet, and an absorbent core disposed at least partially intermediate the topsheet and the backsheet. The topsheet includes a layer of fibers and has a plurality of apertures defined in the layer of fibers. At least some of the apertures have a side wall having a portion disposed an angle in the range of about 55 degrees to about 90 degrees, in the range of about 60 degrees to about 80 degrees, or in the range of about 63 degrees to about 75 degrees, according to the 2D X-Ray CT Scan Test herein.

Abstract: A disposable cleaning cloth includes a low weight substrate having a first face and an opposed second face. The disposable cleaning cloth further includes microfiber yarn sewn to the low weight substrate in a plurality of parallel rows of straight stiches. The straight stitches are substantially flush with the first and second faces of the substrate. A method of making a disposable cleaning cloth includes providing a low weight substrate having a first face and an opposed second face. The method further includes sewing a microfiber yarn to the low weight substrate in a plurality of parallel rows of straight stiches, such that the straight stitches are substantially flush with the first and second faces of the substrate.

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

Abstract: The invention provides a method and system for encrypting and decrypting two-dimensional code mask. The method comprises: generating a target two-dimensional code containing a function pattern and an identification, an initial mask pattern and a key by a two-dimensional code generating device; sending the identification, the initial mask pattern and the key to a server by the two-dimensional code generating device; sending the identification to the server by the two-dimensional code decoding device; receiving the identification by the server; sending the key and the initial mask pattern to the two-dimensional code decoding device by the server according to the identification; receiving by the two-dimensional code decoding device the key and the initial mask pattern sent by the server; and decrypting the encrypted two-dimensional code by the two-dimensional code decoding device according to the key and the initial mask pattern to acquire the target two-dimensional code. The system has high security.

Abstract: The invention provides a detection method and system for characteristic patterns of Han Xin codes. The detection method comprises the steps: binarizing a received two-dimensional code image, scanning the two-dimensional code image line-by-line and column-by-column so as to search out the four vertexes of a Han Xin code region, connecting every two vertexes of the four vertexes so as to form the four boundaries and two diagonal lines of the Han Xin code region, searching out four characteristic line segments starting from the vertexes and having the continuous binarization values 1, 0, 1, 0, 1 from the two diagonal lines and also searching out the end points of the characteristic line segments, and calculating the boundaries and the data bit width of position detection patterns of the Han Xin code according to the start points and the end points of the characteristic line segments.