Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3?-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Abstract: Methods and compositions are described for multi-stage primer extension reactions such as multiplex polymerase chain reactions (PCR) and reverse transcriptase PCR. Primer extension stages are performed in a closed vessel without opening the vessel between stages. The multi-stage primer extension methods and compositions utilize earlier stage primers in an earlier stage and later stage primers in a later stage, wherein the later stage primers are blocked from extension during the earlier stage. The blocked primers of the present technology comprise photocleavable blocking groups and are substantially inactive until the blocking group is cleaved by exposure to ultraviolet light. The blocked primers can be activated by ultraviolet light without opening the vessel.

Abstract: A pre-saturated disposable disinfecting wipe may include a microfiber cloth. The microfiber cloth may include a low weight substrate having a first face and an opposed second face, and microfiber yarn attached to the low weight substrate in a plurality of parallel rows of stitches. The pre-saturated disposable disinfecting wipe further includes a disinfecting formulation saturated in the microfiber cloth. The pre-saturated disposable disinfecting wipe may have a single-touch two-minute wetness coverage of more than 100 in2. A method of manufacturing a pre-saturated disposable disinfecting wipe may include saturating, with a disinfecting formulation, a microfiber cloth.

Abstract: An absorbent article is provided. The absorbent article includes a nonwoven, liquid permeable topsheet, a liquid impermeable backsheet, and an absorbent core disposed at least partially intermediate the topsheet and the backsheet. The topsheet includes a layer of fibers and has a plurality of apertures defined in the layer of fibers. At least some of the apertures have a side wall having a portion disposed an angle in the range of about 55 degrees to about 90 degrees, in the range of about 60 degrees to about 80 degrees, or in the range of about 63 degrees to about 75 degrees, according to the 2D X-Ray CT Scan Test herein.

Abstract: A disposable cleaning cloth includes a low weight substrate having a first face and an opposed second face. The disposable cleaning cloth further includes microfiber yarn sewn to the low weight substrate in a plurality of parallel rows of straight stiches. The straight stitches are substantially flush with the first and second faces of the substrate. A method of making a disposable cleaning cloth includes providing a low weight substrate having a first face and an opposed second face. The method further includes sewing a microfiber yarn to the low weight substrate in a plurality of parallel rows of straight stiches, such that the straight stitches are substantially flush with the first and second faces of the substrate.

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

Abstract: Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

Abstract: The invention provides a method and system for encrypting and decrypting two-dimensional code mask. The method comprises: generating a target two-dimensional code containing a function pattern and an identification, an initial mask pattern and a key by a two-dimensional code generating device; sending the identification, the initial mask pattern and the key to a server by the two-dimensional code generating device; sending the identification to the server by the two-dimensional code decoding device; receiving the identification by the server; sending the key and the initial mask pattern to the two-dimensional code decoding device by the server according to the identification; receiving by the two-dimensional code decoding device the key and the initial mask pattern sent by the server; and decrypting the encrypted two-dimensional code by the two-dimensional code decoding device according to the key and the initial mask pattern to acquire the target two-dimensional code. The system has high security.

Abstract: The invention provides a detection method and system for characteristic patterns of Han Xin codes. The detection method comprises the steps: binarizing a received two-dimensional code image, scanning the two-dimensional code image line-by-line and column-by-column so as to search out the four vertexes of a Han Xin code region, connecting every two vertexes of the four vertexes so as to form the four boundaries and two diagonal lines of the Han Xin code region, searching out four characteristic line segments starting from the vertexes and having the continuous binarization values 1, 0, 1, 0, 1 from the two diagonal lines and also searching out the end points of the characteristic line segments, and calculating the boundaries and the data bit width of position detection patterns of the Han Xin code according to the start points and the end points of the characteristic line segments.

Abstract: Mop assemblies and accessories for cleaning are provided. A mop head includes an elongated body having a length dimension that is greater than a width dimension, the elongated body having a cleaning side and an opposed backside, and a plurality of fabric ribs defining a plurality of channels therebetween, the plurality of fabric ribs extending from the cleaning side of the elongated body.

Abstract: A decoding method includes that when encoding at a sending terminal, for a m-order primitive polynomial P(x), a primitive field element in galois field GF(2m) is represented by ?; a lookup table f(?j) for different power exponents of ? is established, where the value of j is selected from all the integers ranging from 0 to 2m?1, with a total number of 2m; a generator polynomial G(x) is expanded to obtain a polynomial with respect to x, with coefficients being an addition or subtraction of the power exponents of ?; a remainder polynomial R(x), obtained by dividing code word polynomial Q(x) by the generator polynomial G(x), is a polynomial with respect to x, with coefficients being an addition or subtraction of the power exponents of ?; and the coefficients of the generator polynomial G(x) and the remainder polynomial R(x) are both calculated using data found in the lookup table f(?j).

Abstract: The invention provides a decoding method and system for a QR code with one damaged position detection pattern. The decoding method comprises the steps: binarizing a received image containing a QR code pattern and searching for position detection patterns, decoding the QR code pattern through the following method if two position detection patterns are searched out, recording the linear equations of the boundaries of the two position detection patterns and the vertex coordinates of the two position detection patterns and calculating the data bit width of the QR code pattern, searching out a location pattern between the two position detection patterns with the positional relation determined, determining the region where the QR code pattern is located according to the two position detection patterns and the location pattern, partitioning the region where the QR code pattern is located with grids, and decoding the QR code pattern partitioned with grids.

Abstract: A flame resistant fabric is formed from synthetic fibers, such as polyester fibers or non-aramid polyamide fibers. A first flame retardant is incorporated into the fibers by being intimately mixed with the polymer used to form the fibers. A second flame retardant is then surface applied to the fabric or to the yarns used to form the fabric. The fabric has a total flame retardant compound concentration of at least 5000 ppm. In one embodiment, the fabric can be combined with other fabric layers to produce a composite fabric product.

Abstract: A primary localization method for QR codes comprises: S1, binarizing pixel points of a received image containing a QR code; S2, partitioning the image into at least one mesh region; S3, recording the number of black pixel points in each mesh region, assigning the value 1 to mesh regions including black points, and assigning the value 0 to mesh regions not including black points; S4, marking the image as a non-object region; S5, scanning the non-object region line-by-line and column by column and marking the union of the mesh regions continuously with the value 1 for the first time in each line and the mesh regions continuously with the value 1 for the first time in each column as an object region; S6, judging whether the object region is the QR code region, if not, returning to step S5, and if yes, outputting the line numbers and the column numbers.

Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

Abstract: An absorbent article is provided. The absorbent article includes a nonwoven, liquid permeable topsheet, a liquid impermeable backsheet, and an absorbent core disposed at least partially intermediate the topsheet and the backsheet. The topsheet includes a layer of fibers and has a plurality of apertures defined in the layer of fibers. At least some of the apertures have a side wall having a portion disposed an angle in the range of about 55 degrees to about 90 degrees, in the range of about 60 degrees to about 80 degrees, or in the range of about 63 degrees to about 75 degrees, according to the 2D X-Ray CT Scan Test herein.

Abstract: High absorbency mops and associated methods and kits are provided. The mop includes a mob body with at least one outer layer and an inner layer. The outer layer of the mop contains a nonwoven material, and the inner layer contains sodium polyacrylate in an amount of at least one percent by weight of the inner layer.

Abstract: The invention provides a method and system for decoding two-dimensional code using weighted average gray-scale algorithm. The method comprises: segmenting a received two-dimensional code image into bit regions each containing one bit data; presetting a shifting threshold, and shifting four original boundary lines of the bit region outwards by a distance of the shifting threshold to obtain four preset boundary lines; calculating an average gray-scale value of the bit region using the weighted average algorithm by taking an overlapping area between pixels inside the four preset boundary lines and the bit region as a weight; binarizing the two-dimensional code image according to the average gray-scale value of the bit region; and decoding the two-dimensional code image. The influence of the pixels which have an overlapping area with the bit region on the average gray-scale value of the bit region is considered, and the decoding accuracy and success rate are high.