Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered photocatalytic activation of molecular entities in a proximity-dependent manner, which can be actuated within biological systems. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts, and activatable molecular entities incorporating light-responsive moieties that restrict their activity; systems thereof; and methods for catalytically activating the activatable molecular entities via bioluminescence-triggered catalysis.

Abstract: Provided herein are methods, compositions, and kits for decrosslinking formaldehyde cross-linked biological samples such as formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Abstract: Disclosed herein are compositions and methods for decrosslinking formaldehyde cross-linked biological samples such as formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Abstract: Disclosed are an alkali-activated geopolymer coating, and a preparation method and use thereof. The alkali-activated geopolymer coating, is prepared by raw materials including the following components: an aluminosilicate mineral, an alkali activator, and a performance additive; wherein the aluminosilicate mineral comprises fly ash, slag, and metakaolin; the performance additive comprises a polymer powder, titanium dioxide, a silicone defoamer, and a water-reducing admixture; and a mass ratio of the aluminosilicate mineral, the alkali activator, and the performance additive is in a range of 100:(60-80):(4-15).

Abstract: A winding die is disclosed, including: a gear baffle, where a middle portion of the gear baffle is provided with a first opening for placing an iron core post, and the gear baffle is provided with a first surface; and a cylinder, where the cylinder is arranged on the first surface of the gear baffle and connected with the gear baffle. The middle portion of the gear baffle is provided with the first opening for placing the iron core post, the gear baffle is fixedly connected with the cylinder, an external device is connected with a gear, and the cylinder on the gear baffle is driven to rotate around the iron core post by pulling the gear, so that a foil or an insulating material on the cylinder can be wound on the iron core post, finally realizing a foil winding.

Abstract: Provided herein are assays utilizing oxygen-sensitive fluorescent materials for the detection of oxygen. In particular, oxygen-sensitive fluorescent particles are provided for monitoring the oxygen consumption rate and metabolic fitness of living cells.

Abstract: Provided herein are materials and methods for performing bioluminescent assays using a bifunctional probe. In particular, the present disclosure provides compositions and methods for detecting and/or quantifying a biomolecule and/or assaying a cellular process associated with the biomolecule using a bifunctional probe capable of binding the biomolecule and generating a bioluminescent and/or fluorescent signal.

Abstract: Disclosed are an alkali-activated geopolymer coating, and a preparation method and use thereof. The alkali-activated geopolymer coating, is prepared by raw materials including the following components: an aluminosilicate mineral, an alkali activator, and a performance additive; wherein the aluminosilicate mineral comprises fly ash, slag, and metakaolin; the performance additive comprises a polymer powder, titanium dioxide, a silicone defoamer, and a water-reducing admixture; and a mass ratio of the aluminosilicate mineral, the alkali activator, and the performance additive is in a range of 100:(60-80):(4-15).

Abstract: A fastening assembly includes a mounting cylinder frame, a movable part and a turnover part, the mounting cylinder frame is provided with a first vertical beam and a transmission rod, the mounting cylinder is provided with a butting block at the top of the first vertical beam, the transmission rod passes through the butting block, the side wall of the first vertical beam is provided with a bulge, the movable part is connected with the transmission rod, and the turnover part is arranged on the first vertical beam and rotationally connected with the movable part. The turnover part is butted with the bulge, the turnover part is located between the butting block and the movable part, and has a vertical state and a horizontal state. The transmission rod is used to drive the turnover part to switch between the horizontal state or the vertical state.

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. For example, the compounds may be used for in vivo imaging.

Abstract: A mounting bracket includes a bracket body, a locking assembly and support foot. The bracket body is configured for component installation. The locking assembly is arranged on the bracket body, and the support foot is arranged on the bracket body. The support foot is slide along the up and down direction of the bracket body, and the locking assembly is configured to lock the support foot at a preset position. Through the above structure, the support foot is installed on the ground and used to support the bracket body, and the locking assembly locks the support foot at a preset position to correspondingly adjust the height of the bracket body from the ground, so as to adjust the suspension height of the components installed on the bracket body.

Abstract: A regulating device for regulating water consumption of a concealed water tank includes a water tank, a hinged base is fixedly arranged on the inner wall of the water tank, a water separator is hinged on the hinged base. A regulating mechanism is arranged between the hinged base and the water separator, and the regulating mechanism can drive the water separator to rotate and fix to divide the accommodation space of the water tank. Compared with the prior art, the regulating device realizes the variable volume of the water tank by regulating the rotation of the water separator through the regulating mechanism. The structure is simple and convenient for the later repair and maintenance of the water tank.

Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Abstract: Disclosed herein are compounds that can be used to selectively detect nitric oxide in samples. Also disclosed herein are compositions comprising the compounds and methods of detecting nitric oxide using the compounds.

Abstract: Provided herein are polysiloxane matrices and biocompatible films thereof for the detection of oxygen. In particular, oxygen-sensitive fluorophores embedded within polysiloxane matrices are utilized for detection of oxygen consumption rate in living cells.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Abstract: Conjugates comprise a drug or probe, a cell binding agent, and a quinone-containing linker. The quinone-containing linker may be reduced intracellularly to trigger release of the drug or probe. These conjugates may selectively deliver a drug or probe to a site of action of interest for local release of the active drug or probe.