Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

Abstract: Provided herein are polymeric compounds, compositions, and methods for isolation of nucleic acids from a sample.

Abstract: Methods using photothermal nanoparticles in rapid nucleic acid amplification and photothermal nanoparticles include forming a reaction mixture comprising a nucleic acid template, a polymerase enzyme, and a heating media. The heating media comprises a plurality of photothermal nanoparticles suspended in a solution, fabricated on a microchip, or on a surface of a well in a multi-well plate. The photothermal nanoparticles are nanoparticles having a particular geometric shape and comprise a single component or a multi-component. The plurality of photothermal nanoparticles are further categorized by surface plasmon resonance with a resonance wavelength in a particular range to convert energy absorbed from a light source to sufficiently heat a volume of a composition for the transcription of the new nucleic acid strand. The particular geometric shape of the photothermal nanoparticles is selected from the group consisting of: polyhedral, spheroid, torus, and hollow shapes.

Abstract: Provided herein are compositions and methods for purifying and concentrating nucleic acids from large-volume samples. In particular, reagents are provided for non-specifically binding total nucleic acid to a solid surface, separating bound nucleic acid from a large-volume sample, separating nucleic acid from amplification inhibitors, and/or eluting nucleic acids into a small volume amenable to further analysis.

Abstract: The present invention provides compounds and methods of use thereof for assessing target engagement for DNA polymerase theta. In particular, provided herein are tracer compounds comprising a small molecule that binds DNA polymerase theta tethered to a fluorophore, and methods of use thereof in target engagement assays.

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered photocatalytic activation of molecular entities in a proximity-dependent manner, which can be actuated within biological systems. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts, and activatable molecular entities incorporating light-responsive moieties that restrict their activity; systems thereof; and methods for catalytically activating the activatable molecular entities via bioluminescence-triggered catalysis.

Abstract: Disclosed herein are compositions and methods for decrosslinking formaldehyde cross-linked biological samples such as formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Abstract: Provided herein are methods, compositions, and kits for decrosslinking formaldehyde cross-linked biological samples such as formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Abstract: Provided herein are assays utilizing oxygen-sensitive fluorescent materials for the detection of oxygen. In particular, oxygen-sensitive fluorescent particles are provided for monitoring the oxygen consumption rate and metabolic fitness of living cells.

Abstract: Provided herein are materials and methods for performing bioluminescent assays using a bifunctional probe. In particular, the present disclosure provides compositions and methods for detecting and/or quantifying a biomolecule and/or assaying a cellular process associated with the biomolecule using a bifunctional probe capable of binding the biomolecule and generating a bioluminescent and/or fluorescent signal.

Abstract: A fastening assembly includes a mounting cylinder frame, a movable part and a turnover part, the mounting cylinder frame is provided with a first vertical beam and a transmission rod, the mounting cylinder is provided with a butting block at the top of the first vertical beam, the transmission rod passes through the butting block, the side wall of the first vertical beam is provided with a bulge, the movable part is connected with the transmission rod, and the turnover part is arranged on the first vertical beam and rotationally connected with the movable part. The turnover part is butted with the bulge, the turnover part is located between the butting block and the movable part, and has a vertical state and a horizontal state. The transmission rod is used to drive the turnover part to switch between the horizontal state or the vertical state.

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. For example, the compounds may be used for in vivo imaging.

Abstract: A mounting bracket includes a bracket body, a locking assembly and support foot. The bracket body is configured for component installation. The locking assembly is arranged on the bracket body, and the support foot is arranged on the bracket body. The support foot is slide along the up and down direction of the bracket body, and the locking assembly is configured to lock the support foot at a preset position. Through the above structure, the support foot is installed on the ground and used to support the bracket body, and the locking assembly locks the support foot at a preset position to correspondingly adjust the height of the bracket body from the ground, so as to adjust the suspension height of the components installed on the bracket body.

Abstract: A regulating device for regulating water consumption of a concealed water tank includes a water tank, a hinged base is fixedly arranged on the inner wall of the water tank, a water separator is hinged on the hinged base. A regulating mechanism is arranged between the hinged base and the water separator, and the regulating mechanism can drive the water separator to rotate and fix to divide the accommodation space of the water tank. Compared with the prior art, the regulating device realizes the variable volume of the water tank by regulating the rotation of the water separator through the regulating mechanism. The structure is simple and convenient for the later repair and maintenance of the water tank.

Abstract: The present disclosure includes compositions and methods for improved DNA amplification reactions. In particular, the present disclosure provides compositions and methods for hot-start PCR applications using DNA polymerase inhibitors that minimize non-specific DNA amplification by inactivating DNA polymerase at lower temperatures.

Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Abstract: Disclosed herein are compounds that can be used to selectively detect nitric oxide in samples. Also disclosed herein are compositions comprising the compounds and methods of detecting nitric oxide using the compounds.

Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.