Abstract: A bracelet, featuring a leash of adjustable length which extends from the adjustable wristband at a particular point generally at the top of the wrist, with a guitar pick attached to the other end of the leash. When the pick is in use, the leash extends between the thumb and index finger to where the pick is held, and behaves as a normal guitar pick. When the pick is dropped, it swings on the leash so that it is easy to control; the leash can hang completely out of the way down the back of the hand. There is also a storage feature such as a clip to store the pick and leash in a convenient, self-contained bracelet form. The system can be adjusted to accommodate the player's body and preference. It is a low-profile bracelet appropriate for everyday wear.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: Wall panel constructions are disclosed having visually detectable dimensional characteristic imparted to a panel exterior surface as a raised relief area by providing a cabin wall panel assembly with a panel insert having a dimensional characteristic interposed between a panel substrate and a deformable outer panel layer that is deformed under pressure to form the visually detectable raised relief area comprising the dimensional characteristic in the cabin panel exterior surface.

Abstract: A modular, cooling device for providing cool air to a body and/or an external environment comprises a first body portion combining a cooling medium and air passageways therethrough. The first body portion may be detached from second and third body portions and placed in a freezer to refreeze the cooling media, and reattached for further use. The second body portion may include a material that absorbs condensate that forms within the air passageways. The third body portion may include a fan for driving air into the cooling device, through the air passageways and out of the cooling device. When passing through the air passageways, a heat transfer is produced between the passing air and the cooling medium, cooling the passing air. The fan speed may be varied to adjust air flow.

Abstract: The present disclosure provides compositions and methods for the targeted modification of RNA molecules by RNA prime editing. The compositions and methods may be conducted invitro or in vivo within cells (e.g., human cells) for the therapeutic correction of disease-causing mutations and/or installation of motifs or mutations in RNA molecules of interest as a tool for scientific research. The disclosure provides compositions and methods for conducting RNA prime editing of a target RNA molecule (e.g., an RNA transcript) that enables the incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target RNA molecule. The nucleotide change can include a single-nucleotide change, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: A manufacturing tool for forming composite structures includes at least one interchangeable mold template having a plurality of incorporated part shapes, and a backbone that connects to and supports the at least one interchangeable mold template during a process of laying up prepreg composite material to form a plurality of three-dimensional structures corresponding to the plurality of incorporated part shapes.

Abstract: Vehicle cabin structures comprising vehicle structure panels with embedded or associated lighting for producing changing and visually detected illuminated images appearing within the vehicle structure panels are disclosed with methods of their manufacture.

Abstract: The present disclosure provides compositions and methods for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The nucleotide change can include a single-nucleotide change (e.g., any transition or any transversion), an insertion of one or more nucleotides, or a deletion of one or more nucleotides. More in particular, the disclosure provides fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA.

Abstract: A robe garment provided with an integrated mammary support incorporates support system is provided. The robe may be operable to envelop a torso and features a front and a strategically positioned posterior opening. The support system includes bilateral mammary cups, each tethered to a respective cable. These cables are conjoined to belts emanating from the robe's posterior opening and the actuation of these belts facilitates breast elevation, thereby providing tailored support. Additionally, the robe is equipped with interior guide loops for precise cable navigation, exterior belt loops for belt alignment, and a concealing rear cover to enhance aesthetics and ensure user privacy.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: The disclosure provides modified pegRNAs comprising one or more appended nucleotide structural motifs which increase the editing efficiency during prime editing, increase half-life in vivo, and increase lifespan in a cell. Modifications include, but are not limited to, an aptamer (e.g., prequeosim-1 riboswitch aptamer or “evopreQi-1”) or a variant thereof, a pseudoknot (the MMLV viral genome pseudoknot or “Mpknot-1”) or a variant thereof, a tRNA (e.g., the modified tRNA used by MMLV as a primer for reverse transcription) or a variant thereof, or a G-quadruplex or a variant thereof. The disclosure further provides prime editor complexes comprising the modified pegRNAs and having improved characteristics and/or performance, including stability, improved cellular lifespan, and improved editing efficiency.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incoporated into the target DNA molecule.

Abstract: A portable radio may include a radio frequency (RF) transmitter, an RF receiver, and an audio input transducer. A controller may store command messages and speech messages, implement a stand-alone, speech recognition and text-to-speech (TTS) function for the stored command messages and stored speech messages. The controller may also control at least one of an RF transmitter and RF receiver of a remote radio based upon an input command matching one of the stored command messages using the audio input transducer and the stand-alone speech recognition and TTS function, and convert a speech message matching one of the stored speech messages into a text message. The RF transmitter may send the text message to the remote receiver.

Abstract: Compositions and methods are provided herein for conducting prime editing of a target DNA molecule (e.g., a genome) that enables the incorporation of a nucleotide change and/or targeted mutagenesis. The compositions include fusion proteins comprising nucleic acid programmable DNA binding proteins (napDNAbp) and a polymerase (e.g., reverse transcriptase), which is guided to a specific DNA sequence by a modified guide RNA, named an PEgRNA. The PEgRNA has been altered (relative to a standard guide RNA) to comprise an extended portion that provides a DNA synthesis template sequence which encodes a single strand DNA flap which is synthesized by the polymerase of the fusion protein and which becomes incorporated into the target DNA molecule.

Abstract: A system to clean well casing in a downhole well operation. The system comprises an inner collar having flow ports and an out collar having jet ports in fluid communication with the flow ports. The inner collar couples with a section of a tool string and the outer collar rotates about the inner collar in response to fluid flow through the tool string. The inner collar can include a sleeve. The sleeve can be moved from a first position to a second position causing the jet ports to be in fluid communication with the flow ports. The inner collar remains relatively stationary with respect to the rotation of the outer collar. In addition, the jet ports are angled in a way that a portion of force generated by the fluid flow through the jet ports induce rotation of the outer collar in the opposite direction of the fluid flow.

Abstract: Systems and methods for detecting the presence of a target nucleic acid in a sample via a recombinase polymerase amplification (RPA) reaction followed by a FEN1 cleavage detection reaction are disclosed. One aspect of the present disclosure relates to systems involving a sample collection device for collecting a sample and performing an RPA reaction on the sample, followed by the detection of the amplified product via a two-step FEN1 cleavage detection reaction which generates a fluorescent signal indicative of the presence of amplified product.