Abstract: This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato. In further aspects the invention provides novel polynucleotides encoding the tomato thymidine kinase of mutant thereof, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention. In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog nucleoside analog AZT (3?-azido-3?-deoxythymidine) to treat abnormal cell growth.

Abstract: This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitizing cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs. In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa).

Abstract: This invention relates to mutant forms of carboxypeptidase U with increased thermal stability relative to wild-type. In addition to the individual thermal stabilizing mutations identified (at positions 166, 204, 219, 230, 251, 315), the inventors have identified a region (S327-H357) that is crucial to the stability of CPU. The invention relates to nucleic acid encoding such mutant forms and the polypeptides encoded thereby. The invention also relates to methods and materials for making CPU mutants with increased thermal stability relative to wild-type and their use, for example to produce crystals of CPU or proCPU for 3-dimensional structure determination, or in therapy.

Abstract: This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitising cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs. In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa).

Abstract: A heat exchanger including a plurality of longitudinal flat tubes stacked together with space between the broad sides of adjacent tubes, and inlet and outlet collecting tanks. The inlet collecting tank distributes a first medium into the tubes, and has a wall extending around the periphery of one end the stack of flat tubes over a selected longitudinal section, the wall having at least one of an inlet and outlet for a second medium distributed in the space between the stacked tubes. The outlet collecting tank receives the first medium from the tubes. Longitudinal internal inserts in the flat tubes are metallically connected to the broad sides of the associated flat tube. At least one row of cutouts is between the longitudinal ends of the internal inserts and in the selected longitudinal section, with the row of cutouts extending substantially across the broad width of the insert.

Abstract: A cooling system includes a radial fan rotatable by an axis to deliver radial air flow to the cooling system, a plurality of heat exchangers spaced radially from the fan and arranged around the fan in a box-like configuration to receive the radial air flow therefrom, with the heat exchangers bounding an interior space of the cooling system. One of the heat exchangers is provided in the form of a charge air cooler that includes a first flow path for a charge air flow in heat exchange relation with a second flow path for an engine coolant flow of the vehicle. The cooling system further includes a surface associated with the charge air cooler to substantially block the radial air flow from exiting the interior space through the charge air cooler.

Abstract: This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato, pine, rice or thale cress. In further aspects the invention provides novel polynucleotides encoding the plant thymidine kinases, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention. In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog AZT to treat abnormal cell growth.

Abstract: An economically manufactured heat exchanger is provided and includes a plurality of elongated, straight, flat tubes (16), each formed of two identical halves (78,80) joined to each other in mirror image fashion and each having opposed open ends and all arranged in a stack (18). At least one spacer wall (36) is disposed within each tube and extends generally from end to end thereof to define at least two side-by-side first fluid flow paths within a tube. An elongated housing (10) contains the stack and includes spaced headers (20,22) with each header including a tube slot for the adjacent end of each tube (16) in the stack (18). Spacers (26) in the stack separate adjacent tubes in the stack from one another and the spacers include ribs defining a serpentine flow path for a second fluid. An opening (30) is located in the housing (10) near each end thereof and is in fluid communication with the second fluid flow path (28).

Abstract: A heat exchanger including a first header having an inlet therein, a second header, an outlet in one of the first and second headers, and a plurality of flat tubes extending between the first and second headers for carrying a fluid between the first and second headers. A first connector is also provided for connecting a first exterior line to one of the first and second headers, the first connector being proximate and substantially parallel to an end of one of the flat tubes. In a compact cooling system, such heat exchangers may be disposed about a radial fan directing air flow outwardly away from the fan axis. One of a system inlet and a system outlet are connected via the first exterior lines to the first connectors of at least two of the heat exchangers.

Abstract: An air-cooled charge air cooler for vehicles with a coolant-filled pre-cooler oriented in an air collection tank of the charge air cooler. The pre-cooler is sized to contact a majority of the charge air entering the charge air cooler. The pre-cooler has flow paths carrying coolant between manifolds of the pre-cooler and the flow paths define channels therethrough to direct charge air through the pre-cooler and into a cooling grate of the charge cooler. The channels have a depth that allows for a corresponding adjustment in the length of the cooling grate of the charge air cooler while maintaining the overall space requirement for the charge air cooler in a vehicle and meeting the increasing performance requirements for such charge air coolers.

Abstract: A compact cooling system including a radial fan directing air flow outwardly and a plurality of heat exchangers disposed around the radial fan. Each heat exchanger has a plurality of tubes extending between an inlet header and an outlet header, with the headers extending generally in the same direction as the fan axis. A system inlet is connected to the inlet headers and a system outlet is connected to the outlet headers, whereby the length of the connection between each heat exchanger and the system outlet and system inlet is generally the same for each heat exchanger.

Abstract: This invention relates to novel multi-substrate deoxyribonucleoside kinase variants. More specifically the invention provides novel deoxyribonucleoside kinase variants derived from insects or lower vertebrates, in particular from Drosophila melanogaster, from Bombyx mori, or from Xenopus laevis, novel polynucleotides encoding multi-substrate nucleoside kinase variants, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, method of inhibiting pathogenic agents in warm-blooded animals, and pharmaceutical compositions comprising deoxyribonucleoside kinase variants of the invention.

Abstract: An economically manufactured heat exchanger is provided and includes a plurality of elongated, straight, flat tubes (16), each formed of two identical halves (78,80) joined to each other in mirror image fashion and each having opposed open ends and all arranged in a stack (18). At least one spacer wall (36) is disposed within each tube and extends generally from end to end thereof to define at least two side-by-side first fluid flow paths within a tube. An elongated housing (10) contains the stack and includes spaced headers (20,22) with each header including a tube slot for the adjacent end of each tube (16) in the stack (18). Spacers (26) in the stack separate adjacent tubes in the stack from one another and the spacers include ribs defining a serpentine flow path for a second fluid. An opening (30) is located in the housing (10) near each end thereof and is in fluid communication with the second fluid flow path (28).

Abstract: A transmission drives a radial fan about a fan axis in a vehicle cooling unit including a plurality of heat exchangers arranged to surround the radial fan to receive a radial air flow therefrom. A driving pulley, a driven pulley, and a idler pulley of the heat exchangers.

Abstract: An air-cooled charge air cooler for vehicles with a coolant-filled pre-cooler oriented in an air collection tank of the charge air cooler. The pre-cooler is sized to contact a majority of the charge air entering the charge air cooler. The pre-cooler has flow paths carrying coolant between manifolds of the pre-cooler and the flow paths define channels therethrough to direct charge air through the pre-cooler and into a cooling grate of the charge cooler. The channels have a depth that allows for a corresponding adjustment in the length of the cooling grate of the charge air cooler while maintaining the overall space requirement for the charge air cooler in a vehicle and meeting the increasing performance requirements for such charge air coolers.

Abstract: A cooling system includes a radial fan rotatable by an axis to deliver radial air flow to the cooling system, a plurality of heat exchangers spaced radially from the fan and arranged around the fan in a box-like configuration to receive the radial air flow therefrom, with the heat exchangers bounding an interior space of the cooling system. One of the heat exchangers is provided in the form of a charge air cooler that includes a first flow path for a charge air flow in heat exchange relation with a second flow path for an engine coolant flow of the vehicle. The cooling system further includes a surface associated with the charge air cooler to substantially block the radial air flow from exiting the interior space through the charge air cooler.

Abstract: A box-like cooling system (10) is provided for use in a vehicle. The system (10) include a plurality of heat exchangers (16, 18, 20) in a box-like arrangement around a radial fan (12) that directs a radial air flow through the cores (42) of the heat exchangers (16, 18, 20). The system (10) includes a front wall (22) having an inlet opening (60) for the air flow drawn by the fan (12), and a plurality of dynamic air flow ports (66) that are closed when the fan (12) is in an operating state providing the radial cooling air flow to the heat exchangers (16, 18, 20), and are open when the fan (12) is in a non-operating state and there is a dynamic air pressure on the front wall (22) produced by forward movement of the vehicle so as to allow a cooling air flow through the ports (66) into interior space (26) of the cooling system (10) and then through the cores (42) of the heat exchangers (16, 18, 20).

Abstract: A compact cooling system for a vehicle includes an assembly of heat exchangers (10), (12), (14) and (16) connected to each other to provide a box-like configuration. Each heat exchanger (10), (12), (14), (16) includes a core with headers (20), (22) at each end in fluid communication with spaced, flattened tubes (18) with fins (24) extending between the tubes in each of the cores. One of the cores (10) is generally horizontal and located at the upper side of the box-like configuration. A radial discharge fan (42) is located within the box-like configuration and receives cooling air and directs the same radially outwardly through the cores to cool fluid passing through the tubes (18) of the cores. An equalization tank (60), (80) is mounted on the box-like configuration in overlying, spaced relation to a substantial portion of the surface of the heat exchanger (18) so as to be in the path of cooling air merging from the heat exchanger (10) to divert the same.

Abstract: An exhaust gas heat exchange system for an internal combustion engine (10) includes an intake manifold (12) including an inlet (16) for recirculating exhaust gases and an exhaust manifold (28) having at least one inlet (30) for receiving exhaust gas from the engine (10) and an outlet (36), (60), for discharging exhaust gas and spaced from the inlets (30). An exhaust gas heat exchanger (32) includes a first flow path having an inlet (50) connected to the exhaust manifold (32) to receive exhaust gas therefrom, an outlet (52) for discharging cooled exhaust therefrom and a second flow path in heat exchange relation with the first flow path for receipt of a coolant whereby exhaust gas flowing in the first flow path may be cooled.

Abstract: A heat exchanger including a first header having an inlet therein, a second header, an outlet in one of the first and second headers, and a plurality of flat tubes extending between the first and second headers for carrying a fluid between the first and second headers. A first connector is also provided for connecting a first exterior line to one of the first and second headers, the first connector being proximate and substantially parallel to an end of one of the flat tubes. In a compact cooling system, such heat exchangers may be disposed about a radial fan directing air flow outwardly away from the fan axis. One of a system inlet and a system outlet are connected via the first exterior lines to the first connectors of at least two of the heat exchangers. 655.