Abstract: A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic—MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.

Abstract: Herein are provided a method for producing a multispecific antibody comprising the steps of providing a mammalian cell expressing the antibody, transfecting said mammalian cell with an expression vector comprising an expression cassette encoding a polypeptide of the antibody that has a domain crossover, cultivating the transfected cell and recovering the antibody from the cell or the cultivation medium and thereby producing the multispecific antibody.

Abstract: Method for monitoring and controlling current distribution in load circuits of an installation control system of a technical installation, wherein a predetermined and constant output voltage is provided by a clocked power supply and distributed to the load circuits, where load circuits are protected by a switch actuated by a controller, a variation of the current in each load circuit is measured during a learning phase, a significant current profile with an associated tolerance range is derived and associated with the respective load circuit from the measured current variation which is continuously monitored by the control unit and a check is performed to determine whether a power capacity limit is reached by the clocked power supply while operate the installation, and the current consumed load circuits is reduced and/or switched off by actuating switches in load circuits in which a current variation exceeds an upper limit of the tolerance range.

Abstract: High throughput cultivation systems are used in pharmaceutical research and development. In this connection, samples are taken and analyzed for important parameters using external analysis. The results of the analysis serve to assess the cultivation process and provide important information about the process. Especially with cultivations carried out in parallel, the manual effort of sample preparation is great and can lead to errors. In order to avoid the need for sampling and thus to minimize the errors, a method is described in the present patent application which makes desired target parameters accessible in the form of soft sensors by means of previously recorded process variables. Herein is described a method for determining process-relevant parameters in CHO processes (Chinese hamster ovary) in high-throughput cultivations, in particular glucose, lactate and the live cell density or the live cell volume.

Abstract: A method and a circuit for complying with specified maximum values for output parameters a power supply unit includes at least a non-floating switch converter, an output voltage control unit, a current limiter and a switch element, wherein actual values of the current and voltage outputs of the power supply unit are measured continuously, where an evaluation unit calculates actual output power values of the power supply unit from the actual measured values of the output current and voltages, and subsequently compares at least the respective actually measured values of the output current and the respective actually calculated output power values with specified maximum values such that if at least one of the specified maximum values is exceeded by an actually measured value of the output current and/or by an actually calculated output power value, a current flow in the power supply unit is then interrupted by the evaluation unit.

Abstract: A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic—MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.

Abstract: Herein are provided a method for producing a multispecific antibody comprising the steps of providing a mammalian cell expressing the antibody, transfecting said mammalian cell with an expression vector comprising an expression cassette encoding a polypeptide of the antibody that has a domain crossover, cultivating the transfected cell and recovering the antibody from the cell or the cultivation medium and thereby producing the multispecific antibody.

Abstract: A method and a circuit for complying with specified maximum values for output parameters a power supply unit includes at least a non-floating switch converter, an output voltage control unit, a current limiter and a switch element, wherein actual values of the current and voltage outputs of the power supply unit are measured continuously, where an evaluation unit calculates actual output power values of the power supply unit from the actual measured values of the output current and voltages, and subsequently compares at least the respective actually measured values of the output current and the respective actually calculated output power values with specified maximum values such that if at least one of the specified maximum values is exceeded by an actually measured value of the output current and/or by an actually calculated output power value, a current flow in the power supply unit is then interrupted by the evaluation unit.

Abstract: The invention relates to a tool for crushing coke in drums with of high-pressure water jets, which has a housing 2 with a feed system 4 for high-pressure water, and a housing wall 3 with outwardly directed boring and cutting nozzles 5a, 5b, out of the openings 7 of which high-pressure water jets 28 exit, as well as flow channels 4g, 4h, which connect the feed system 4 with the boring and cutting nozzles 5a, 5b. So that the opening of the boring and cutting nozzles is permanently protected and kept free of deposits of coke or the like, the opening of the boring or cutting nozzle is respectively closable by a one- or multi-part flap, which is adjustable between a closed position and an open position.

Abstract: The invention relates to a tool for crushing coke, and includes a housing 2 that, in an operating state, is fastened to a drill rod, and in which is arranged at least one cutting nozzle 10 for cutting and a drilling nozzle 9 for drilling coke by means of a water jet, and a control for supplying water flowing through the housing 2 under pressure for drilling or cutting, wherein the control for switching the tool 1 from a drilling mode to a cutting mode and vice versa includes a control device 7 that is rotatable about a longitudinal axis of the housing 2 to switch the tool 1, wherein the drilling or cutting mode is set depending on the angular position of the control device 7. To obtain a clear understanding of whether the tool 1 is in drilling or cutting mode, at least one position element 38, 39 is arranged on the control device 7 that displays the angular position of the control device 7 in the drilling and/or cutting mode of the tool 1 through a window 18 in the housing 2.

Abstract: A saddle, in particular a clamping saddle for plastic pipes, containing a branch projection, a saddle piece, center axes (X), (Y) and (Z), and a groove for the arrangement of a seal, wherein the inner cheek of the groove is arranged, throughout the course of the groove, perpendicular to the inner surface of the saddle piece.

Abstract: A converter for an electrical machine having a plurality of phase lines for connecting the electrical machine. For each phase line the converter has a half-bridge with a first semiconductor switch is configured to electrically connect at least one of the phase lines intermittently to a first supply line of the converter, and a second semiconductor switch configured to electrically connect the phase line intermittently to a second supply line of the converter. The converter is configured for operation in a first energy recovery limiting mode in which at least two of the first semiconductor switches are at least intermittently on simultaneously.

Abstract: The invention relates to a tool for crushing coke, and includes a housing 2 that, in an operating state, is fastened to a drill rod, and in which is arranged at least one cutting nozzle 10 for cutting and a drilling nozzle 9 for drilling coke by means of a water jet, and a control for supplying water flowing through the housing 2 under pressure for drilling or cutting, wherein the control for switching the tool 1 from a drilling mode to a cutting mode and vice versa includes a control device 7 that is rotatable about a longitudinal axis of the housing 2 to switch the tool 1, wherein the drilling or cutting mode is set depending on the angular position of the control device 7. To obtain a dear understanding of whether the tool 1 is in drilling or cutting mode, at least one position element 38, 39 is arranged on the control device 7 that displays the angular position of the control device 7 in the drilling and/or cutting mode of the tool 1 through a window 18 in the housing 2.

Abstract: The invention relates to a tool for crushing coke in drums with of high-pressure water jets, which has a housing 2 with a feed system 4 for high-pressure water, and a housing wall 3 with outwardly directed boring and cutting nozzles 5a, 5b, out of the openings 7 of which high-pressure water jets 28 exit, as well as flow channels 4g, 4h, which connect the feed system 4 with the boring and cutting nozzles 5a, 5b. So that the opening of the boring and cutting nozzles is permanently protected and kept free of deposits of coke or the like, the opening of the boring or cutting nozzle is respectively closable by a one- or multi-part flap, which is adjustable between a dosed position and an open position.

Abstract: A tool for crushing coke using high-pressure water is attached to a drilling rod through which water flows at high pressure. The tool further a control device for directing the water into either drilling nozzles or cutting nozzles depending on the rotational position of the control device. A switching element driven by water pressure engages the control device to cause the control the device to rotate from one rotational position to another rotational position, thereby changing the tool from cutting to drilling or vice versa.

Abstract: A saddle, in particular a clamping saddle for plastic pipes, containing a branch projection, a saddle piece, centre axes (X), (Y) and (Z), and a groove for the arrangement of a seal, wherein the inner cheek of the groove is arranged, throughout the course of the groove, perpendicular to the inner surface of the saddle piece.

Abstract: This invention relates to a device (13) for the distribution of firing pulses, a circuit arrangement (10) for sequence control of power regulators (20) with this device (13) for the distribution of firing pulses and a process for the sequence control of power regulators (20) for operation with this circuit arrangement (10). A circuit arrangement of the above type and components for such a circuit arrangement and a process for operation of the circuit arrangement is provided, enabling better usage of the components, in particular of the device (12) for the generation of firing pulses. This is made possible through the invented device (13) for the distribution of firing pulses or an invented circuit arrangement (10) with this device (13).

Abstract: The present invention relates to a novel process for preparing solid, small particles comprising melamine cyanurate in essentially crystalline form. A concentrated aqueous dispersion is prepared of a mixture of cyanuric acid and melamine and selected surfactants are added to the dispersion.

Abstract: Embodiments of the present invention provide a method and system for managing life-cycles of a “software as a service” (SaaS) software application. In one embodiment, a method comprises installing the SaaS software application in a system landscape on a computer server, the system landscape containing at least an application server and a database (DB) server; separating system data from customer data and storing them in different databases; creating a virtual machine (VM) image for the system landscape, the VM image to include the databases containing system data and exclude the databases containing customer data; deploying the SaaS software application to one or more computer servers by loading VMs based on the VM image; when the SaaS software application need to be upgraded to a new version, preparing a new VM image with the new version of the SaaS software application and using the new VM image.

Abstract: The invention relates to a method for programming and/or diagnosis of a memory-programmable controller, having at least one memory-programmable function component. For programming, a predetermined programming system is used. In the context of this programming system variables are predetermined, and information exchange sequences are used for the programming. Results of the programming are output during at least one programming mode via an output device, and input information is at least in part stored permanently in memory.