Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Abstract: A method for making controlled-release ammonium phosphate fertilizer has the following acts of: cracking, pulverizing, blending, aging, drying, and adding release-controlling materials into an ammonium phosphate slurry during preparing processes or adding release-controlling materials in achieved ammonium phosphate powder. Selectively, sulfuric acid is added into the mixture of the release-controlling materials and the ammonium phosphate slurry or ammonium phosphate powder to acidify the mixture. In this method, the release-controlling material combines with the ammonium phosphate by chemical bonding to control the release of nitrogen and phosphorus nutrients to enhance the use efficiency and elongate fertilization effect of the controlled-release ammonium phosphate fertilizer.

Abstract: A method for making controlled-release ammonium phosphate fertilizer has the following acts of: cracking, pulverizing, blending, aging, drying, and adding release-controlling materials into an ammonium phosphate slurry during preparing processes or adding release-controlling materials in achieved ammonium phosphate powder. Selectively, sulfuric acid is added into the mixture of the release-controlling materials and the ammonium phosphate slurry or ammonium phosphate powder to acidify the mixture. In this method, the release-controlling material combines with the ammonium phosphate by chemical bonding to controll the release of nitrogen and phosphorus nutrients to enhance the use efficiency and elongate fertilization effect of the controlled-release ammonium phosphate fertilizer.