Abstract: The present disclosure provides a hard mask structure for integrated circuit manufacturing and a method for manufacturing an integrated circuit device, which relate to a field of pattern transfer technology in a chip manufacturing process.

Abstract: A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Abstract: The disclosure discloses an expression cassette for expressing a gene including overlapping open reading frames in an insect cell and an application thereof. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; the overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a native or engineered intron with splicing activity in the insect cell, the intron includes ATG or the intron is located between any two adjacent nucleotides in ATG. A recombinant adeno-associated virus vector including the expression cassette of the disclosure regulates relative expressions of VP1, VP2, and VP3 proteins, and relative expressions of Rep78 and Rep52 proteins by using a designed intron sequence and through an intron splicing function for large-scale production of rAAV.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: Provided are a preparation method and system for a recombinant adeno-associated virus (rAAV) and a recombinant bacmid. The method comprises: first reconstructing a recombinant bacmid containing a recombinant baculovirus genome that produces essential functional elements for an rAAV, at least one of the essential functional elements being inserted into the N-terminal or C-terminal of a locus of an essential gene of the recombinant baculovirus genome; and then transfecting the obtained recombinant bacmid containing the recombinant baculovirus genome that produces the rAAV into a host cell line for culturing to prepare an rAAV. Compared with recombinant baculoviruses obtained by conventional Tn7 recombinant preparations of recombinant bacmid, the recombinant baculovirus obtained by inserting a core element containing Cap, Rep and ITR into two sides of a baculovirus essential gene has a more stable rAAV serial passage production level in a cell and has a higher rAAV yield.

Abstract: The present disclosure discloses a deep ultraviolet lithography method, a lithography pattern and a semiconductor structure, which relates to the field of deep ultraviolet lithography technology. The deep ultraviolet lithography method includes: dividing, when a depth of field of the deep ultraviolet lithography is less than a height of the step of the substrate, a pattern to be photoetched into at least two portions according to a distribution situation of the step, where each of the at least two portions corresponds to an on-step pattern or an off-step pattern of the step; corresponding the at least two portions of the pattern to be photoetched onto at least two masks respectively; and simultaneously baking and developing the exposed at least two masks after the at least two masks are exposed sequentially.

Abstract: The present disclosure relates to a method of obtaining a nanoscale line by using a laser, including: forming a dielectric layer and an amorphous silicon layer on a substrate sequentially; irradiating a mask plate by using the laser to perform a silicon crystallization in a partial region of the amorphous silicon layer, where a grain boundary of a polycrystalline silicon formed by the silicon crystallization in the partial region of the amorphous silicon layer is determined by a spacing between holes with a regular shape on the mask plate; performing a planarization process on the grain boundary of the polycrystalline silicon of the amorphous silicon layer; removing the grain boundary by using a corrosion solution to form a grain boundary trench; and obtaining the nanoscale line on the substrate by using the grain boundary trench.

Abstract: A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: A micro core-drilling testing system for high-pressure environment, including: a pressure chamber, a micro core-drilling testing platform and a control device. The micro core-drilling testing platform includes a lower tray, a middle tray, a hydraulic system, a stroke cylinder, a rock sample plate, a rock sample block, a pressure compensation device and a drilling rig. The drilling rig is configured to carry out core-drilling operation. The hydraulic system is configured to provide power for the drilling rig. The pressure compensation device is configured to balance the pressure inside the pressure chamber and the hydraulic system.

Abstract: The disclosure discloses an expression cassette for expressing a gene including overlapping open reading frames in an insect cell and an application thereof. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; the overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a native or engineered intron with splicing activity in the insect cell, the intron includes ATG or the intron is located between any two adjacent nucleotides in ATG. A recombinant adeno-associated virus vector including the expression cassette of the disclosure regulates relative expressions of VP1, VP2, and VP3 proteins, and relative expressions of Rep78 and Rep52 proteins by using a designed intron sequence and through an intron splicing function for large-scale production of rAAV.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: A frame door with a split connection frame is provided, including a split connection frame. The split connection frame includes a first connection frame which is provided in a frame door and a second connection frame which is provided in an other frame door. The first connection frame is provided with a slide rail groove, the second frame is provided with a slide plate which engages with the slide rail groove. The slide rail groove has a C-shaped cross section so as to wrap edges of the slide plate.

Abstract: The disclosure belongs to the field of genetic engineering and discloses an expression cassette of a gene including overlapping open reading frames and an application thereof in an insect cell. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a first intron and a second intron with splicing activity in the insect cell, the first intron and the second intron share a 5? part or a 3? part, and the artificially constructed sequence does not include a translation start codon; during a post-transcriptional processing process, through an alternative splicing function of the first intron and the second intron, a translation start codon AUG can be formed.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: Provided are a preparation method and system for a recombinant adeno-associated virus (rAAV) and a recombinant bacmid. The method comprises: first reconstructing a recombinant bacmid containing a recombinant baculovirus genome that produces essential functional elements for an rAAV, at least one of the essential functional elements being inserted into the N-terminal or C-terminal of a locus of an essential gene of the recombinant baculovirus genome; and then transfecting the obtained recombinant bacmid containing the recombinant baculovirus genome that produces the rAAV into a host cell line for culturing to prepare an rAAV. Compared with recombinant baculoviruses obtained by conventional Tn7 recombinant preparations of recombinant bacmid, the recombinant baculovirus obtained by inserting a core element containing Cap, Rep and ITR into two sides of a baculovirus essential gene has a more stable rAAV serial passage production level in a cell and has a higher rAAV yield.

Abstract: An assembled door frame is provided, including multiple of side frames spliced together, where every adjacent two of the side frames spliced at right angle are connected via a corner connector assembly. The corner connector assembly is arranged inside the every adjacent two of the side frames spliced at right angle. The corner connector assembly includes a corner connector and fixing bolts, and one of the every adjacent two of side frames spliced at right angle is fixed on the corner connector by the fixing bolts. In the embodiments, the side frames are spliced directly together through the respective corner connector assemblies each including the corner connector and the fixing bolts. On one hand, the corner connector and the fixing bolts are each disposed within the corresponding side frame, such that the original external structure of the side frames will not be changed after the side frames are spliced together, thereby reducing assembly errors of the door frame caused by using the connecting structure.

Abstract: A frame door with a split connection frame is provided, including a split connection frame. The split connection frame includes a first connection frame which is provided in a frame door and a second connection frame which is provided in an other frame door. The first connection frame is provided with a slide rail groove, the second frame is provided with a slide plate which engages with the slide rail groove. The slide rail groove has a C-shaped cross section so as to wrap edges of the slide plate. By using the split connection frame design, and using the sliding connection solution of slide rail groove and slide plate, on the one hand, the operation is convenient, time and labor are saved by the preset slide track during mounting process. On the other hand, the frame door is enable to be positioned in the mounting process via the preset slide track, thereby avoiding the assembly error caused by improper operation when using the existing mounting method and improving the assembly accuracy.

Abstract: Liver-specific promoters and application thereof are provided. A set of small-size recombinant nucleic acid sequences for regulating high specific expression of genes in a liver system are provided. Compared with the currently reported other sequences of similar size, the said recombinant regulatory sequence fragment has the advantage that the ability of driving expression of reporter gene and human coagulation factor FVIII in the liver system is significantly enhanced, thereby being suitable for recombinant adeno-associated virus (rAAV)-mediated gene therapy.