Abstract: A baculovirus vector and a use thereof in the preparation of a recombinant adeno-associated virus (rAAV) in an insect cell are provided. The baculovirus vector includes an exogenous gene expression cassette and a stable sequence. The stable sequence is located at a site 5 kb or less from the exogenous gene expression cassette, and the stable sequence is a conserved noncoding element (CNE) sequence or a nucleocapsid assembly-essential element (NAE) sequence. When an insect cell is infected with a recombinant baculovirus (rBV) constructed in this way, after multiple continuous passages, production levels of the rBV and the rAAV still remain relatively stable.

Abstract: An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5? end to a 3? end: a first promoter at least used to drive gene transcription of the first ORF; a 5? part of a gene of the first ORF; an intron; and a 3? part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Abstract: A micro core-drilling testing system for high-pressure environment, including: a pressure chamber, a micro core-drilling testing platform and a control device. The micro core-drilling testing platform includes a lower tray, a middle tray, a hydraulic system, a stroke cylinder, a rock sample plate, a rock sample block, a pressure compensation device and a drilling rig. The drilling rig is configured to carry out core-drilling operation. The hydraulic system is configured to provide power for the drilling rig. The pressure compensation device is configured to balance the pressure inside the pressure chamber and the hydraulic system.

Abstract: The disclosure discloses an expression cassette for expressing a gene including overlapping open reading frames in an insect cell and an application thereof. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; the overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a native or engineered intron with splicing activity in the insect cell, the intron includes ATG or the intron is located between any two adjacent nucleotides in ATG. A recombinant adeno-associated virus vector including the expression cassette of the disclosure regulates relative expressions of VP1, VP2, and VP3 proteins, and relative expressions of Rep78 and Rep52 proteins by using a designed intron sequence and through an intron splicing function for large-scale production of rAAV.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: A frame door with a split connection frame is provided, including a split connection frame. The split connection frame includes a first connection frame which is provided in a frame door and a second connection frame which is provided in an other frame door. The first connection frame is provided with a slide rail groove, the second frame is provided with a slide plate which engages with the slide rail groove. The slide rail groove has a C-shaped cross section so as to wrap edges of the slide plate.

Abstract: The disclosure belongs to the field of genetic engineering and discloses an expression cassette of a gene including overlapping open reading frames and an application thereof in an insect cell. The expression cassette includes from 5? to 3? and operably linked: a promoter capable of driving transcription in the insect cell; an artificially constructed sequence; overlapping open reading frames missing only a first translation start codon; wherein the artificially constructed sequence includes a first intron and a second intron with splicing activity in the insect cell, the first intron and the second intron share a 5? part or a 3? part, and the artificially constructed sequence does not include a translation start codon; during a post-transcriptional processing process, through an alternative splicing function of the first intron and the second intron, a translation start codon AUG can be formed.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: Provided are a preparation method and system for a recombinant adeno-associated virus (rAAV) and a recombinant bacmid. The method comprises: first reconstructing a recombinant bacmid containing a recombinant baculovirus genome that produces essential functional elements for an rAAV, at least one of the essential functional elements being inserted into the N-terminal or C-terminal of a locus of an essential gene of the recombinant baculovirus genome; and then transfecting the obtained recombinant bacmid containing the recombinant baculovirus genome that produces the rAAV into a host cell line for culturing to prepare an rAAV. Compared with recombinant baculoviruses obtained by conventional Tn7 recombinant preparations of recombinant bacmid, the recombinant baculovirus obtained by inserting a core element containing Cap, Rep and ITR into two sides of a baculovirus essential gene has a more stable rAAV serial passage production level in a cell and has a higher rAAV yield.

Abstract: A frame door with a split connection frame is provided, including a split connection frame. The split connection frame includes a first connection frame which is provided in a frame door and a second connection frame which is provided in an other frame door. The first connection frame is provided with a slide rail groove, the second frame is provided with a slide plate which engages with the slide rail groove. The slide rail groove has a C-shaped cross section so as to wrap edges of the slide plate. By using the split connection frame design, and using the sliding connection solution of slide rail groove and slide plate, on the one hand, the operation is convenient, time and labor are saved by the preset slide track during mounting process. On the other hand, the frame door is enable to be positioned in the mounting process via the preset slide track, thereby avoiding the assembly error caused by improper operation when using the existing mounting method and improving the assembly accuracy.

Abstract: An assembled door frame is provided, including multiple of side frames spliced together, where every adjacent two of the side frames spliced at right angle are connected via a corner connector assembly. The corner connector assembly is arranged inside the every adjacent two of the side frames spliced at right angle. The corner connector assembly includes a corner connector and fixing bolts, and one of the every adjacent two of side frames spliced at right angle is fixed on the corner connector by the fixing bolts. In the embodiments, the side frames are spliced directly together through the respective corner connector assemblies each including the corner connector and the fixing bolts. On one hand, the corner connector and the fixing bolts are each disposed within the corresponding side frame, such that the original external structure of the side frames will not be changed after the side frames are spliced together, thereby reducing assembly errors of the door frame caused by using the connecting structure.

Abstract: Liver-specific promoters and application thereof are provided. A set of small-size recombinant nucleic acid sequences for regulating high specific expression of genes in a liver system are provided. Compared with the currently reported other sequences of similar size, the said recombinant regulatory sequence fragment has the advantage that the ability of driving expression of reporter gene and human coagulation factor FVIII in the liver system is significantly enhanced, thereby being suitable for recombinant adeno-associated virus (rAAV)-mediated gene therapy.

Abstract: A micro core-drilling testing system for high-pressure environment, including: a pressure chamber, a micro core-drilling testing platform and a control device. The micro core-drilling testing platform includes a lower tray, a middle tray, a hydraulic system, a stroke cylinder, a rock sample plate, a rock sample block, a pressure compensation device and a drilling rig. The drilling rig is configured to carry out core-drilling operation. The hydraulic system is configured to provide power for the drilling rig. The pressure compensation device is configured to balance the pressure inside the pressure chamber and the hydraulic system.

Abstract: A method for producing a recombinant adeno-associated virus (rAAV) and a recombinant baculovirus virus, the method including: (1) infecting an insect host packaging cell line with a corresponding recombinant baculovirus integrated with an rAAV genome ITR-GOI (gene of interest flanked by AAV inverted terminal repeats) and an AAV Cap gene or AAV Rep gene; (2) culturing the host packaging cell line infected with the recombinant baculovirus, so as to produce the recombinant adeno-associated virus; and (3) separating and purifying the recombinant adeno-associated virus obtained in (2).

Abstract: A method for producing recombinant adeno-associated virus, the method including: (1) transforming a gene of interest (GOI) into a recombinant baculovirus, where the recombinant baculovirus has a genome integrated with AAV Rep gene, Cap gene, and rAAV genome ITR-GOI that are needed in the production of the rAAV; and where the ITR-GOI is linked to expression cassette of the Cap gene and the Rep gene by a 5? terminal nucleic acid segment or a 3? terminal nucleic acid segment; (2) infecting host insect larvae with the recombinant baculovirus prepared in (1), such that the rAAV is produced in vivo in the host insect larvae; and (3) lysing the host insect larvae obtained in (2), and extracting and purifying the rAAV.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: A recombinant baculovirus, including: an adeno-associated virus Rep gene, an adeno-associated virus Cap gene, and an recombinant adeno-associated virus genome ITR-GOI (gene of interest) flanked by rAAV inverted terminal repeats (ITR). The ITR-GOI includes a 5? terminal nucleic acid fragment and a 3? terminal nucleic acid fragment. The ITR-GOI is linked to an expression cassette of the Cap gene and an expression cassette of the Rep gene through the 5? terminal nucleic acid fragment and the 3? terminal nucleic acid fragment, respectively.

Abstract: Among other things, embodiments of the present disclosure can help to automatically generate images displaying activity-based information and distribute such images to other users, such as members of a social network. The image may be modified based on activity data associated with other users and/or from other activity sensors.

Abstract: A method for monolithic integration of a hyperspectral image sensor is provided, which includes: forming a bottom reflecting layer on a surface of the photosensitive region of a CMOS image sensor wafer; forming a transparent cavity layer composed of N step structures on the bottom reflecting layer through area selective atomic layer deposition processes, where N=2m, m?1 and m is a positive integer; and forming a top reflecting layer on the transparent cavity layer. With the method, non-uniformity accumulation due to etching processes in conventional technology is minimized, and the cavity layer can be made of materials which cannot be etched. Mosaic cavity layers having such repeated structures with different heights can be formed by extending one-dimensional ASALD, such as extending in another dimension and forming repeated regions, which can be applied to snapshot hyperspectral image sensors, for example, pixels, and greatly improving performance thereof.