Abstract: A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

Abstract: A container-based application management method and apparatus are disclosed. A container-based serverless computing system is configured such that an application instance is allowed to be in one of an online state and a low-power state at runtime. In response to scaling down an application, at least one first application instance of the application in the online state is caused to enter the low-power state; and in response to scaling up the application, at least one second application instance of the application in the low-power state is caused to enter the online state. In this way, not only the cost of the application instance can be reduced, but also rapid elastic scaling can be performed on the application instance.

Abstract: A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

Abstract: Systems and methods for determining regions of proteins that contribute to self-association of the protein are provided. Methods for modifying the self-association of concentrated protein formulations are also provided.

Abstract: Methods of predicting an in vivo serum concentration of an antibody with a post-translational modification of interest after administration of the antibody are provided, as are methods for predicting a subject's exposure to post-translational variants of the antibody. The methods include predicting a percentage of the antibody with the post-translational modification of interest using an in vivo rate constant determined for the post-translational modification, and multiplying the predicted percentage of the antibody with the post-translational modification of interest by the in vivo concentration of the antibody to determine the concentration of the antibody with the post-translational modification of interest.

Abstract: The present invention provides stable liquid pharmaceutical formulations comprising a human bispecific antibody that specifically binds to human MUC16 and human CD3. In certain embodiments, the formulations contain, in addition to the bispecific antibody, a buffer, a surfactant, and a sugar. The pharmaceutical formulations of the present invention exhibit a substantial degree of antibody stability upon stress and storage.

Abstract: Embodiments provide for methods of predicting glycation percentage of an amino acid in a therapeutic biomolecule. In one example, a method of predicting a glycation percentage of an amino acid in a biomolecule includes determining a first set of rates for a de-glycation reaction for a first set of temperatures, inferring a second set of one or more rate(s) for the de-glycation reaction for a second set of temperatures, and using the second set of one or more rate(s) to predict the glycation percentage at any temperature corresponding to the second set of temperatures and over any time duration. Also provided are methods for maintaining a glycation percentage of an amino acid within a predetermined glycation percentage range over a shelf-life of a therapeutic biomolecule, and methods for either reducing or increasing a potency of a therapeutic biomolecule in a subject at a time of administration.

Abstract: A bionic visual navigation control system for autonomous aerial refueling docking includes: a tanker/receiver bottom layer control module, a multi-wind disturbances hose-drogue stable control module, an enable and select module, a close-range bionic vision relative navigation module, and a receiver relative position precise control module. A bionic visual navigation control method for autonomous aerial refueling docking is also provided. The present invention aims at improving the reliability, anti-interference and accuracy of the close-range relative navigation in the autonomous air refueling docking stage, and designs a matching relative position accurate control method with control switch, thereby improving the accuracy of close-range navigation and control, thereby promoting the successful realization of probe-and-drogue autonomous aerial refueling and improving the autonomy of UAVs.

Abstract: Methods of predicting an in vivo serum concentration of an antibody with a post-translational modification of interest after administration of the antibody are provided, as are methods for predicting a subject's exposure to post-translational variants of the antibody. The methods include predicting a percentage of the antibody with the post-translational modification of interest using an in vivo rate constant determined for the post-translational modification, and multiplying the predicted percentage of the antibody with the post-translational modification of interest by the in vivo concentration of the antibody to determine the concentration of the antibody with the post-translational modification of interest.

Abstract: A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

Abstract: The present disclosure discloses a circuit board and a manufacturing method thereof. The manufacturing method of the circuit board comprises: forming an electroplated coating on a board body of the circuit board; performing image transfer on the board body; drilling the board body after image transfer to remove a copper layer, adjacent to the two sides of a gold finger, on the circuit board, thereby forming a strip; performing forward and reverse routing towards directions away from each other respectively at the two sides of each gold finger to form a first routing tape and a second routing tape, wherein the first routing tape and the second routing tape are connected to the two ends of the strip respectively; and removing burrs on a surface of the board body through an etching process.

Abstract: A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

Abstract: The disclosure provides methods of characterizing a sample of oligonucleotides of interest using liquid chromatography and mass spectrometry.

Abstract: A robust and general fabrication/manufacturing method is described herein for the fabrication of periodic three-dimensional (3D) hierarchical nanostructures in a highly scalable and tunable manner. This nanofabrication technique exploits the selected and repeated etching of spherical particles that serve as resist material and that can be shaped in parallel for each processing step. The method enables the fabrication of periodic, vertically aligned nanotubes at the wafer scale with nanometer-scale control in three dimensions including outer/inner diameters, heights/hole-depths, and pitches. The method was utilized to construct 3D periodic hierarchical hybrid silicon and hybrid nanostructures such as multi-level solid/hollow nanotowers where the height and diameter of each level of each structure can be configured precisely as well as 3D concentric plasmonic supported metal nanodisk/nanorings with tunable optical properties on a variety of substrates.

Abstract: The present invention provides methods and systems for isotyping and quantification of antibodies based on immunocapture and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. These antibodies are induced by the administration of pharmaceutical products. The immunocapture method comprises contacting samples with a solid support, wherein the pharmaceutical product has been cross-linked directly to the solid support. The MS analysis includes conducting peptide mapping, selecting unique peptides and fragment ions to generate MRM (multiple reaction monitoring) transitions, optimizing collision energy, and determining a LLOQ (lower limit of quantification).

Abstract: A hollow-core antiresonant fiber (HC-ARF) with nested supporting rings (NSRs) has a fiber structure that includes from the inside out a fiber core, a first silica antiresonant ring (SARR), a first air antiresonant ring (AARR), a second SARR, a second AARR and an external silica wall. The fiber structure further includes a first NSR within the first AARR and a second NSR within the second AARR. The HC-ARF with NSRs has advantages and benefits of low confined loss (CL), large bandwidth, simple structure and very good bending characteristics. Therefore, the application fields of HC-ARF are greatly expanded.

Abstract: A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs.

Abstract: A hollow-core antiresonant fiber (HC-ARF) with nested supporting rings (NSRs) has a fiber structure that includes from the inside out a fiber core, a first silica antiresonant ring (SARR), a first air antiresonant ring (AARR), a second SARR, a second AARR and an external silica wall. The fiber structure further includes a first NSR within the first AARR and a second NSR within the second AARR. The HC-ARF with NSRs has advantages and benefits of low confined loss (CL), large bandwidth, simple structure and very good bending characteristics. Therefore, the application fields of HC-ARF are greatly expanded.

Abstract: A method for characterizing or quantifying one or more proteins in visible and/or sub-visible particles formed in a sample by detecting the at least one visible or sub-visible particle in the sample, isolating and capturing the at least one visible or sub-visible particle to identify a presence of a protein, and using a mass spectrometer to characterize the protein.

Abstract: The nondestructive determination of core size of a hollow-core photonic bandgap fiber (HC-PBF) using Fabry-Perot (FP) interference is performed with an apparatus including a tunable laser source (TLS), a 1×2 single-mode (SM) coupler, an SM collimator, a six-axis translation stage, an optical detector, and an oscilloscope. The light from the TLS passes through the 1×2 SM coupler and the SM collimator to perpendicularly enter two parallel air-SiO2 interfaces of the core of the fiber and is reflected, while the TLS is tuned from one wavelength to another. Then the reflected spectrum is guided to the optical detector, where its interference intensity is converted into voltage intensity to be displayed at the oscillator and fitted with a least-squares method to obtain the distance between the two air-SiO2 interfaces. The core size of the fiber can be obtained by rotating the fiber and repeating the procedure at multiple angular positions.