Abstract: There is a growing need for enhancements to human workhorse cell lines (e.g., HEK293 cells) critical for synthesis of specific therapeutic products such as viral vectors for gene therapies. To that end, aspects of the disclosure are drawn to genetically engineered cells and Methods of boosting bioproduction capacity in mammalian cells.

Abstract: The invention provides methods for producing crystalline an anti-PD-1 monoclonal antibody (mAb), wherein the mAb is pembrolizumab or a pembrolizumab variant, comprising (1) mixing a solution comprising (a) the mAb, (b) polyethylene glycol (PEG), and (c) an additive selected from the group consisting of: caffeine, theophylline, 2? deoxyguanosine-5?-monophosphate, a bioactive gibberellin, and a pharmaceutically acceptable salt of said bioactive gibberellin, to form a crystallization solution, (2) incubating the crystallization solution for a period of time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1 mAb from the solution. In specific embodiments, the PEG is PEG 3350 and the additive is caffeine. The invention also relates to the novel anti-human PD-1 mAb crystals produced by the methods described herein.

Abstract: The present disclosure provides a magnetic resonance (MR)-guided charged particle beam radiotherapy device, and an electromagnetic (EM) steering parameter commissioning method aiming at target spots. The device of the present disclosure includes: a charged particle beam EM steering device, a charged particle beam sequentially passing through a multi-stage EM steering coil set to generate a parallel charged particle beam in the same direction as a main magnetic field of MR, and an MR imaging device, including an upper and a lower group of main magnetic field coils. According to the present disclosure, the charged particle beams parallel to the main magnetic field of MR and aiming at preset targets spots can be generated, which greatly reduces the effect of the Lorentz force on the charged particle beams and enhance their dosimetric advantages, thereby improving dose delivery accuracy of the charged particle beams to target spots under MR guidance.

Abstract: The technology of this application relates to an encoding method, an encapsulation method, a display method, an apparatus, and an electronic device. The encoding method includes obtaining a to-be-encoded image, determining N encoding scales (N is an integer greater than 1) for the image, determining an encoding parameter corresponding to each of the N encoding scales, to obtain N groups of encoding parameters, and encoding the image N times by using a preset single-scale encoder based on the N groups of encoding parameters to obtain N groups of bitstream data. In this way, the image is encoded to obtain the bitstream data with a small data amount (namely, the bitstream data with a low encoding scale), so that when a network fluctuates, the bitstream data corresponding to the image can arrive at a decoder side with a higher probability, thereby ensuring smoothness of video playing.

Abstract: The invention provides methods for producing crystalline an anti-PD-1 monoclonal antibody (mAb), wherein the mAb is pembrolizumab or a pembrolizumab variant, comprising (1) mixing a solution comprising (a) the mAb, (b) polyethylene glycol (PEG), and (c) an additive selected from the group consisting of: caffeine, theophylline, 2? deoxyguanosine-5?-monophosphate, a bioactive gibberellin, and a pharmaceutically acceptable salt of said bioactive gibberellin, to form a crystallization solution, (2) incubating the crystallization solution for a period of time sufficient for crystal formation; and (3) optionally harvesting the crystalline anti-PD-1 mAb from the solution. In specific embodiments, the PEG is PEG 3350 and the additive is caffeine. The invention also relates to the novel anti-human PD-1 mAb crystals produced by the methods described herein.

Abstract: A tumor radiotherapy planning design method and apparatus, an electronic device, and a computer storage medium are provided, including: obtaining a current optimization parameter vector set, and calculating a current cost function value; randomly correcting the current optimization parameter vector set to generate alternative optimization parameter vector sets; then performing planning parameter optimization, and calculating corresponding total cost function values; determining current optimal or suboptimal alternative optimization parameter vector sets according to the total cost function values, sampling to update the current optimization parameter vector set and the current cost function value according to the total cost function values of the current alternative optimization parameter vector sets, and then performing an iteration repeatedly until a convergence condition is satisfied; and outputting an optimal optimization parameter vector set after the iteration, determining planning parameters, and calcu

Abstract: A magnetic resonance imaging (MRI) system can include a gapped degenerate birdcage magnetic resonance imaging (MRI) radio frequency (RF) coil. The gapped degenerate birdcage MRI RF coil is tuned to a degenerate mode and comprises a row of RF coil elements and a mechanical gap. The RF coil elements may also be referred to as channels or meshes. The row extends circumferentially around an axis of a cylindrical-like former, and mechanical gap separates a pair of directly neighboring RF coil elements in the row. A transformer, a partial overlap, or the like minimizes inductive coupling between the pair of directly neighboring RF coil elements.

Abstract: A non-volatile storage apparatus includes non-volatile memory cells, word lines connected to the non-volatile memory cells, and a control circuit connected to the word lines and the memory cells. The word lines include data word lines and dummy word lines. Memory cells connected to data word lines are configured to store host data. Memory cells connected to dummy word lines do not store host data. The control circuit is configured to erase, program and read the memory cells. Errors from threshold voltage up-shifting in the memory cells connected to dummy word lines is prevented by adjusting the voltage applied to dummy word lines.

Abstract: A low-power self-biased slew rate enhancement circuit includes a self-bias control circuit outputting a bias voltage based on the output voltage of an input voltage detection circuit; the input voltage detection circuit is connected to the self-bias control circuit to detect the magnitude of a differential input voltage; and a slew rate control circuit is used to provide a source current or a sink current to the output end of an operational amplifier when the voltage at the differential input voltage ends exceeds a turn-on voltage, otherwise, no additional current is generated and no slew rate enhancement effect is generated.

Abstract: A lamp includes a light source, a reflecting mirror, an internal lens and a wall element. The reflecting mirror is configured to reflect light rays emitted by the light source to the internal lens, the light rays sequentially pass through the internal lens and the wall element and exit. The internal lens includes a main body, a first protruding part and a second protruding part, and both of the first protruding part and the second protruding part abut the wall element, so that a gap is formed between the main body of the internal lens and the wall element.

Abstract: The present disclosure provides a method of manufacturing a semiconductor structure and a semiconductor structure. The method of manufacturing the semiconductor structure includes: providing an initial structure, where the initial structure includes a laminated structure and a plurality of capacitor holes formed in the laminated structure, and a bottom electrode is formed in each of the capacitor holes; forming a hard mask layer, where the hard mask layer covers a top surface of the initial structure; and partially etching the hard mask layer through an etching gas, to form a plurality of first opening, where the etching gas includes a first gas, and the first gas includes a nitrogen atomic-containing and/or hydrogen atomic-containing gas, to avoid a combination reaction between the first gas and a material of the bottom electrode.

Abstract: In some embodiments, the present disclosure relates to a multi-turn (MT) birdcage magnetic resonance imaging (MRI) radio-frequency (RF) coil. The MT birdcage MRI RF coil includes a first conductive ring, a second conductive ring, and a plurality of conductive rungs. Each of the plurality of conductive rungs includes a first end coupled to the first conductive ring, and a second end coupled to the second conductive ring. At least one of the first conductive ring and the second conductive ring includes more than one turn. The first conductive ring, the second conductive ring, and the plurality of conductive rungs form a plurality of meshes.

Abstract: A super-resolution single-objective light-sheet optical microscopy system and method, and a related imaging system, are configured to generate two light sheets that are guided to a single objective. The single objective is configured to allow the two light sheets to transmit through same and interfere with each other to generate structured light fringe regions. The single objective is further configured to receive a fluorescence signal that is recorded by a fluorescence detection module.

Abstract: The present disclosure is directed to a balun package comprising a set of inductive baluns for a magnetic resonance imaging (MRI) radio frequency (RF) coil. A first resonant circuit and a second resonant circuit each comprise an inductor. A first decoupling circuit leg is between and shared by the first and second resonant circuits and includes one or more decoupling inductors and/or one or more decoupling capacitors. A first cable bundle is wound to form the inductor of the first resonant circuit or is otherwise spaced from and inductively coupled to the inductor of the first resonant circuit.

Abstract: In some embodiments, the present disclosure relates to a radio frequency (RF) surface coil for a magnetic resonance imaging (MRI) system. The RF surface coil includes a rigid lower member, at least one flexible upper member mechanically coupled to the rigid lower member, and one or more RF coil elements housed by the rigid lower member and the at least one flexible upper member. The at least one flexible upper member is dimensioned and manipulable to substantially conform the one or more RF coil elements to a portion of a patient anatomy to be imaged by the MRI system.

Abstract: A method for forming a memory includes: forming a bit line structure and a capacitor contact layer, where the bit line structure includes a bit line, a bit line cap layer and a bit line isolation layer, and the capacitor contact layer covers part of a side wall of the bit line isolation layer; forming a stop layer covering the side wall of the bit line isolation layer; forming a capacitor landing layer covering a top surface of the capacitor contact layer; and etching the bit line isolation layer by using the stop layer as an etch stop layer to form an air gap in the bit line isolation layer. Probability of occurrence of a short circuit between the capacitor landing layer and a bit line is reduced.

Abstract: The present disclosure provides a magnetic resonance (MR)-guided charged particle beam radiotherapy device, and an electromagnetic (EM) steering parameter commissioning method aiming at target spots. The device of the present disclosure includes: a charged particle beam EM steering device, a charged particle beam sequentially passing through a multi-stage EM steering coil set to generate a parallel charged particle beam in the same direction as a main magnetic field of MR, and an MR imaging device, including an upper and a lower group of main magnetic field coils. According to the present disclosure, the charged particle beams parallel to the main magnetic field of MR and aiming at preset targets spots can be generated, which greatly reduces the effect of the Lorentz force on the charged particle beams and enhance their dosimetric advantages, thereby improving dose delivery accuracy of the charged particle beams to target spots under MR guidance.

Abstract: A mask includes a frame, at least one mask sheet, and a shielding plate. The frame includes a plurality of borders. The borders are connected end to end in sequence to form the frame with a first hollow region. A mask sheet includes a pattern region and non-pattern regions. The pattern region includes at least one evaporation hole. The shielding plate includes a plurality of shielding strips. The plurality of shielding strips are arranged crosswise to form a plurality of second hollow regions. Orthogonal projections of the second hollow regions on a plane perpendicular to a thickness direction of the frame are located within a range of an orthogonal projection of the first hollow region on the plane. An inner edge of an orthogonal projection of the frame on the plane is located within a range of an orthogonal projection of the shielding plate on the plane.

Abstract: The invention relates to the technical field of intelligent construction and maintenance, and relates to a non-contact visual monitoring system and method for a flexible protective structure against rockfall disasters; it comprises a hardware system and a data analysis system, and the hardware system uses a multipoint distributed high-resolution high-speed camera to capture dynamic image sequences of a protective structure under the rockfall impact in a non-contact mode; the data analysis system comprises an impact deformation state full-field tracking module and a multipoint impact large deformation extraction module, and the impact deformation state full-field tracking module captures spatio-temporal changes of impact deformation of the protective system by adopting a full-field optical flow method, constructs a two-dimensional velocity amplitude distribution diagram and performs full-field spatio-temporal tracking of large deformation of the system.

Abstract: A display substrate and a mask plate assembly includes a base substrate, a pixel defining layer, a functional layer, a cathode layer, and a covering layer that are sequentially stacked in a direction away from the base substrate, wherein the size of orthographic projection of the functional layer on the base substrate is jointly determined by the size of a display area and a first minimum distance. The first minimum distance is the minimum distance in a direction from the display area to a non-display area and between the boundary of the display area and the boundary of the functional layer, and is determined by a first shadow width of the functional layer extending from the display area to the non-display area. The first shadow width is the maximum width of a shadow area formed within a preset region when the functional layer is vapor deposited in the preset region.