Patents by Inventor Xudong Ye

Xudong Ye has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS TO IMPROVE SITE-DIRECTED INTEGRATION FREQUENCY
    Publication number: 20220162625
    Abstract: The present disclosure relates to compositions and methods for improving site-directed integration frequency in the genome of plant cells.
    Type: Application
    Filed: November 10, 2021
    Publication date: May 26, 2022
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: John BRADLEY, Larry A. GILBERTSON, Ervin NAGY, Christine SHYU, Peizhen YANG, Xudong YE
  • METHODS FOR PLANT TRANSFORMATION USING SPECTINOMYCIN SELECTION
    Publication number: 20210246459
    Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
    Type: Application
    Filed: December 10, 2020
    Publication date: August 12, 2021
    Inventors: Brian J. Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
  • METHODS AND COMPOSITIONS FOR OBTAINING MARKER-FREE TRANSGENIC PLANTS
    Publication number: 20210163977
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Application
    Filed: October 23, 2020
    Publication date: June 3, 2021
    Inventors: Xudong Ye, Larry A. Gilbertson, Shishieh Huang, Susan J. Johnson, Michael W. Petersen, David Walters, Paul S. Chomet
  • GENOME EDITING IN PLANTS
    Publication number: 20210054390
    Abstract: Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.
    Type: Application
    Filed: May 24, 2019
    Publication date: February 25, 2021
    Inventors: Yurong Chen, Annie Saltarikos, Jianping Xu, Xudong Ye
  • Methods for plant transformation using spectinomycin selection
    Patent number: 10907167
    Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: February 2, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Brian J. Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
  • VECTORS AND METHODS FOR IMPROVED PLANT TRANSFORMATION EFFICIENCY
    Publication number: 20210010011
    Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
    Type: Application
    Filed: July 16, 2020
    Publication date: January 14, 2021
    Inventors: Xudong Ye, Larry A. Gilbertson, Michael W. Petersen
  • METHOD OF MERISTEM EXCISION AND TRANSFORMATION
    Publication number: 20200392519
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Application
    Filed: June 17, 2020
    Publication date: December 17, 2020
    Inventors: Erik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • Use of multiple transformation enhancer sequences to improve plant transformation efficiency
    Patent number: 10865418
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: December 15, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Xudong Ye
  • USE OF NON-AGROBACTERIUM BACTERIAL SPECIES FOR PLANT TRANSFORMATION
    Publication number: 20200385746
    Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
    Type: Application
    Filed: June 24, 2020
    Publication date: December 10, 2020
    Inventors: Xudong Ye, Junjiang Shen, Edward Williams
  • SITES FOR PLASTID TRANSFORMATION
    Publication number: 20200362357
    Abstract: Compositions and methods for plastid transformation and regeneration or development of transplastomic plants, plant cells, plant parts, and seeds are provided. Target sequences for plastid transformation, together with defined sequences of a first homology arm and a second homology arm are provided for the production of recombinant plastid transformation constructs that target specific sites within the plastid genome for transformation, together with methods of using such constructs.
    Type: Application
    Filed: December 18, 2017
    Publication date: November 19, 2020
    Inventor: Xudong Ye
  • Vectors and methods for improved plant transformation efficiency
    Patent number: 10752908
    Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: August 25, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Larry A. Gilbertson, Michael W. Petersen
  • Use of non-bacterial species for plant transformation
    Patent number: 10724042
    Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: July 28, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Junjiang Shen, Edward Williams
  • Method of meristem excision and transformation
    Patent number: 10717983
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: July 21, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Erik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • COMPOSITIONS AND METHODS FOR GENOME EDITING IN PLANTA
    Publication number: 20200080096
    Abstract: This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.
    Type: Application
    Filed: September 6, 2019
    Publication date: March 12, 2020
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Stanislaw FLASINSKI, Elysia KRIEGER, Ervin NAGY, Krishna SRIDHARAN, Xudong YE
  • METHODS FOR PLANT TRANSFORMATION USING SPECTINOMYCIN SELECTION
    Publication number: 20190382777
    Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
    Type: Application
    Filed: April 24, 2019
    Publication date: December 19, 2019
    Inventors: Brian J. Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
  • METHODS AND COMPOSITIONS FOR OBTAINING MARKER-FREE TRANSGENIC PLANTS
    Publication number: 20190345507
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Application
    Filed: March 20, 2019
    Publication date: November 14, 2019
    Inventors: Xudong Ye, Larry A. Gilbertson, Shihshieh Huang, Susan J. Johnson, Michael W. Petersen, David Walters, Paul S. Chomet
  • METHODS AND COMPOSITIONS FOR USE IN GENOME MODIFICATION IN PLANTS
    Publication number: 20190211344
    Abstract: The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.
    Type: Application
    Filed: June 27, 2017
    Publication date: July 11, 2019
    Inventors: Elysia K. KRIEGER, Richard J. LAWRENCE, Zarir E. VAGHCHHIPAWALA, Jianping XU, Xudong YE
  • VECTORS AND METHODS FOR IMPROVED PLANT TRANSFORMATION EFFICIENCY
    Publication number: 20190211346
    Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
    Type: Application
    Filed: January 8, 2019
    Publication date: July 11, 2019
    Inventors: Xudong Ye, Larry A. Gilbertson, Michael W. Petersen
  • Methods and compositions for obtaining marker-free transgenic plants
    Patent number: 10240165
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: March 26, 2019
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Xudong Ye, Larry A. Gilbertson, Shihshieh Huang, Susan J. Johnson, Michael W. Petersen, David Walters, Paul S. Chomet
  • Vectors and methods for improved plant transformation efficiency
    Patent number: 10202611
    Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: February 12, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Larry A. Gilbertson, Michael W. Petersen