Abstract: A tandem DNA element capable of enhancing protein synthesis efficiency, in particular, the nucleic acid construct is formed by an IRES enhancer (such as ScBOI1, ScFLO8, ScNCE102, ScMSN1, KlFLO8, KlNCE102, KlMSN1, KlBOI1) derived from eukaryotic cells (such as yeast), a ? sequence, and a yeast-specific Kozak sequence in tandem. The use of the nucleic acid construct in a yeast-based in vitro biosynthesis system (such as a yeast-based in vitro protein synthesis system) can significantly improve protein synthesis efficiency.

Abstract: The present invention provides a polymer molecule, a monomeric structure and a polymeric structure comprising same, a related product, a preparation method, and uses. A polymer blocking the binding to a receptor is formed by the polymerization effect of the polymer molecule, thus effectively increasing the binding capability with respect to a virus. The polymer molecule is characterized in that: multiple binding-blocking molecular units used for blocking the binding between the virus and a cell receptor are polymerized into the polymeric structure.

Abstract: What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.

Abstract: An in-vitro biosynthesis reaction apparatus and an in-vitro biosynthesis method. The in-vitro biosynthesis reaction apparatus comprises: a reaction body, which is used for containing a protein synthesis reaction liquid, so as to carry out an in-vitro biosynthesis reaction, wherein an opening part is arranged on the reaction body, and the opening part is provided with a feeding port used for enabling the synthesis reaction liquid to enter the reaction body and a discharging port allowing a reaction product to be discharged; and a partition part, which is used for incomplete separation of the internal space of the reaction body.

Abstract: Provided are a display substrate and a manufacturing method therefor, and a display device. The display substrate includes: a base substrate including a display area and a frame area located on at least one side of the display area; a plurality of shift registers arranged in cascade in the frame area; a plurality of jumper terminals located between the shift registers and the display area and including first jumper terminals and second jumper terminals; a plurality of transfer terminals located between the shift registers and the display area, and located on a side of the layer where the first jumper terminals are located away from the base substrate; and an insulating layer located in the display area and the frame area, located between a layer where the second jumper terminals are located and a layer where the plurality of transfer terminals are located, and including a channel.

Abstract: Provided is a metal oxide thin film transistor. The metal oxide thin film transistor includes: a gate, a gate insulator layer, a metal oxide semiconductor layer, a source, a drain, and a first insulator layer that are successively stacked on a base substrate. The first insulator layer is in contact with the metal oxide semiconductor layer. The first insulator layer is an inorganic insulator layer containing silicon and oxygen, and an atomic percentage of oxygen contained in the first insulator layer is greater than 50%. An atomic percentage of oxygen contained in the metal oxide semiconductor layer is greater than 45%.

Abstract: An endogenous dynamic defense architecture-based multi-objective service function chain deployment method solves a problem of multi-objective deployment by constructing an endogenous dynamic defense architecture, in which a basic mode includes using moving target defense to ensure the security of VNFs, and an enhanced mode includes using mimic defense to perform security protection on the VNFs; in a construction module, a sub-pool division algorithm is proposed to divide a heterogeneous replica pool into a plurality of sub-pools, and VNFs are selected from the sub-pools so as to constitute a heterogeneous replica set; in a scheduling module, a replica VNF dynamic scheduling deployment algorithm is proposed, a deployment set is selected from the heterogeneous replica set for deployment, and is sent to a processing module; the input module replicas an input and distributes same to the processing module.

Abstract: Provided are a fusion protein comprising a Pab1 element and an eIF4G element and a preparation method therefor. The fusion protein can improve in-vitro translation efficiency. A constitutive or inducible promoter (for example, pKlPGK1) may also be inserted in front of eIF4G in the fusion protein for increasing in-vitro protein synthesis ability.

Abstract: An efficient parallelization and deployment method of a multi-objective service function chain based on a CPU+DPU platform solves the problem of multi-objective deployment by constructing a heterogeneous computing architecture composed of an orchestrator and a server based on a CPU+DPU structure; the orchestrator is responsible for receiving an SFC request from a network operator; an SFC deployment algorithm based on deep reinforcement learning is operated, including a parallel strategy, a VNF topological order strategy and a DPU processing strategy to obtain an optimal deployment scheme of each request; then a resource management module is invoked to manage resources; and finally, a driver module is invoked to transmit the deployment scheme to a server for placement, and the server completes the deployment of SFC by using the CPU or the DPU respectively according to the deployment scheme.

Abstract: A display driving module, a display driving method, and a display device are provided. The display driving module includes a clock signal line, a clock signal generating circuit and a gate driving circuit, where the gate driving circuit includes multiple stages of gate driving units; the clock signal generating circuit is electrically connected to the clock signal line and is configured to generate at least two clock signals and provide different clock signals to the clock signal line in a time-sharing manner; the gate driving unit is electrically connected to the clock signal line and configured to generate a gate driving signal according to the clock signals on the clock signal line; when potentials of the clock signals are valid voltages, the potentials of different clock signals are different.

Abstract: Provided is a polypeptide tag. The amino acid sequence of the polypeptide tag is Xaa1Xaa2Xaa3PHDYNXaa4Xaa5Xaa6 (SEQ ID NO: 37), wherein in the formula, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are each independently an amino acid or none. The polypeptide tag is used for labeling a target protein. In a second aspect, provided is a polypeptide fusion protein, comprising the following two structures: (1) any polypeptide tag according to the first aspect, and (2) a target protein connected to the polypeptide tag. Also provided are an in vitro cell-free protein synthesis system and an application thereof in in vitro protein synthesis. By constructing the polypeptide tag and a target protein as a fusion protein, the expression of the labeled target protein can be effectively increased without removing the polypeptide tag.

Abstract: A tandem DNA element capable of enhancing protein synthesis efficiency, in particular, the nucleic acid construct is formed by an IRES enhancer (such as ScBOI1, ScFLO8, ScNCE102, ScMSN1, KlFLO8, KlNCE102, KlMSN1, KlBOI1) derived from eukaryotic cells (such as yeast), a ? sequence, and a yeast-specific Kozak sequence in tandem. The use of the nucleic acid construct in a yeast-based in vitro biosynthesis system (such as a yeast-based in vitro protein synthesis system) can significantly improve protein synthesis efficiency.

Abstract: What provided is a method for regulating in vitro biosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among numerous nucleases, and performing down-regulation or knocking-out on one of the five nucleases (e.g., EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of an in vitro protein synthesis system.

Abstract: Provided is biological magnetic microsphere, comprising magnetic microsphere body. Magnetic microsphere body comprises, on outer surface thereof, at least one polymer having linear backbone and side chain. End of linear backbone fixes to outer surface of magnetic microsphere body, other ends of polymer unattach to outer surface of magnetic microsphere body. Biotin links to terminal end of side chain of polymer of biological magnetic microsphere. Further provided are modification to, preparation method for, use of biological magnetic microsphere. Biological magnetic microsphere can be handled and used conveniently, rapidly disperse and rapidly precipitate in solution without need to use large-scale experimental equipment such as high-speed centrifuge, can be connected via biotin with purification element having selectivity (such as avidin, affinity protein, polypeptide/protein tag, etc.

Abstract: A biomagnetic microsphere and a preparation method and a method for protein isolation and purification therefor. The outer surface of a magnetic microsphere body of the biomagnetic microsphere has at least one liner polymer with a branched chain; one end of the linear polymer with a branched chain is covalently coupled to the outer surface of the magnetic microsphere body, and other parts are free on the outer surface of the magnetic microsphere body; a backbone of the linear polymer is a polyolefin backbone, and no cross-linking agent is required in the backbone forming process of the linear polymer. The prepared biomagnetic microsphere can implement efficient elution of target proteins and effectively reduce the retention time and retention ratio of the target proteins, and it is easy to operate and widely used.

Abstract: A display driving module, a display driving method, and a display device are provided. The display driving module includes a clock signal line, a clock signal generating circuit and a gate driving circuit, where the gate driving circuit includes multiple stages of gate driving units; the clock signal generating circuit is electrically connected to the clock signal line and is configured to generate at least two clock signals and provide different clock signals to the clock signal line in a time-sharing manner; the gate driving unit is electrically connected to the clock signal line and configured to generate a gate driving signal according to the clock signals on the clock signal line; when potentials of the clock signals are valid voltages, the potentials of different clock signals are different.

Abstract: Provided is a method for quantitatively co-expressing multiple proteins in an in vitro cell-free protein synthesis system, comprising the following steps: (1) establishing a standard curve of the relationship between standard protein concentration and luminescence value; (2) creating a vector containing a target protein gene, and obtaining an in vitro protein synthesis system; (3) establishing a curve of the relationship between the target protein concentration and the vector concentration; (4) calculating the concentration or/and the concentration ratio of the vector quantitatively co-expressing multiple target proteins; (5) quantitatively co-expressing the target proteins.

Abstract: Provided are an ADH protein mutant and the use thereof. Compared with a wild-type ADH protein, the mutant is capable of (i) enhancing the expression purity, efficiency and yield of exogenous proteins in an in-vitro cell-free synthesis system; and/or (ii) reducing the binding ability of the mutant protein to Ni medium.

Abstract: Provided are a fusion protein and a preparation method therefor. The fusion protein can improve in-vitro translation efficiency. A constitutive or inducible promoter (for example, pK1PGK1) is inserted in front of eIF4G in the fusion protein for increasing in-vitro protein synthesis ability.

Abstract: This disclosure relates to systems and methods for managing network expansion. A method includes receiving an indicator that identifies a goal, identifying a target organization at an online social networking service to satisfy the goal, ranking members of the online social networking service that are also members of the target organization according to their respective ability to influence satisfaction of the goal, determining a connection path to one of the higher ranked members, and recommending, to a member, a connection to a next member identified by the connection path.