Abstract: Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace said gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.

Abstract: Provided are a fusion protein and a construction method thereof. The fusion method consists of: a Haemophilus influenzae protein D and a Hin47 (Htra) protein. The fusion protein can serve as a protein vehicle for a Haemophilus influenzae polysaccharide-protein conjugate vaccine, thereby increasing immunogenicity of a polysaccharide antigen.

Abstract: The present invention relates to a blower vacuum device having a housing with an air inlet and an air outlet, a motor accommodated in the housing, a fan driven by the motor, and a plurality of blowing accessories adapted to be connected to the air outlet respectively when the suction or blower device works. These different blowing accessories can perform various cleaning functions, thus meeting the cleaning demands in different places or in a specific area.

Abstract: The invention provides an immunogenic composition for preventing pneumococcal diseases, comprising PspA-MRX1, PspA-EF5668, PspA-EF3296 and PlyL460D, wherein the amount of each component is 10-100 ?g/ml; the immunogenic composition is prepared by adding to aluminum adjuvant the corresponding dosages of the four stock solutions PspA-mRX1, PspA-EF5668, PspA-EF3296 and PlyL460D, and mixing them homogeneously; the immunogenic composition can prevent infection and invasion by Streptococcus pneumonia, covering more than 95% of the strains in clinic. The immunogenic composition has a wide application in the prevention of pneumonia, and is suitable for large-scale production in industry for its simple preparation method, low production cost, and short production cycle.

Abstract: The embodiments relates to garden blower, including: an enclosure, including a main body part located on back end and a blowing pipe located on front end and extending axially, the enclosure disposed with an air inlet and an air outlet communicated with external environment; a power device, connected to the enclosure; a fan component, driven by the power device and generating airflows; the fan component includes at least two-level fans, further includes a first-level fan and a second-level fan axially disposed front and back, the garden blower includes a first air inlet passage allowing entrance of the airflows generated by the first-level fan and a second air inlet passage allowing entrance of the airflows generated by the second-level fan, and the airflows entering the first air inlet passage and the airflows entering the second air inlet passage are converged into the blowing pipe and are blown to the outside.

Abstract: The present invention provides a titanium ligand-modified black phosphorus and the preparation method and use thereof. The titanium ligand-modified black phosphorus is a complex of black phosphorus and a titanium ligand having a structure represented by formula (I): wherein in the formula (I), R1 comprises C1-C6 alkyl, or phenyl optionally further substituted with 0 to 5 groups each independently selected from halogen atom, C1-C6 alkyl, nitro, hydroxy, amino or C1-C3 alkoxy; the C1-C6 alkyl or C1-C3 alkoxy is optionally further substituted with 0 to 3 groups each independently selected from halogen atom, nitro, hydroxy, amino, methyl, ethyl or n-propyl. The titanium ligand-modified black phosphorus of the present invention is not likely oxidized without changing inherent properties of the black phosphorus, and the antioxidant capacity is greatly enhanced.

Abstract: A blower includes a housing having an air inlet; a motor; an axial fan, driven by the motor to rotate along a fan axis and generate air flow, and the axial fan comprising a hub and impeller; a blowing tube, comprising an air outlet for the air exiting; and a duct, comprising a guiding cone which is located between the axial fan and the air outlet, and used for guiding the air flow generated by the axial fan to move to the air outlet, wherein a size of the motor is configured in a manner that the motor cannot be contained in the guiding cone, the housing and the blowing tube are internally provided with an air passage, the motor is completely contained in the air passage of the housing, and the motor and the duct are located on two opposite sides of the axial fan.

Abstract: A pouring metallic plug stick clamping mechanism for cast steel wheels. The mechanism includes: a cast iron frame, a plurality of grooves, a centrally located opening in the frame, a first white ceramic ball, a second white ceramic ball, a third white ceramic ball, a retaining spring, a pressure regulating bolt, a control press button, a metallic plug stick, and a blocking iron piece.

Abstract: A blower, comprising: a housing comprising an air inlet, a motor disposed in the housing, an axial fan configured to be driven to rotate about a fan axis and generate an air flow by the motor, a blowing tube configured to be coupled to the housing and comprising an air outlet, a duct configured to guide the air flow to the air outlet, the housing and the blowing tube comprises an air passage which comprises an upstream region between the inlet and the axial fan and a downstream region between the axial fan and the air outlet, the motor is disposed in the upstream region and the duct is disposed in the downstream region.

Abstract: A method for enhancing immunogenicity of an epitope peptide of an HPV antigen, the method including: assembling a gene of an HPV antigen into a gene of HBc, exogenously expressing a resulting assembled gene to acquire a fusion protein, allowing the fusion protein to automatically assemble to form a virus-like particle including the HPV antigen on a surface of the virus-like particle, to obtain HBc-HPV virus-like particle. A virus-like particle capable of expressing the antigen peptide of HPV acquired by the method. The virus-like particle includes a HBc-L2 fusion protein. A genome for encoding the HBc-L2 fusion protein is represented by SEQ ID NO. 3. A method for preparing an HPV vaccine includes using the virus-like particle.

Abstract: The invention provides an immunogenic composition for preventing pneumococcal diseases, comprising PspA-MRXI, PspA-EF5668, PspA-EF3296 and PlyL460D, wherein the amount of each component is 10-100 ?g/ml; the immunogenic composition is prepared by adding to aluminum adjuvant the corresponding dosages of the four stock solutions PspA-mRXI, PspA-EF5668, PspA-EF3296 and PlyL460D, and mixing them homogeneously; the immunogenic composition can prevent infection and invasion by Streptococcus pneumonia, covering more than 95% of the strains in clinic. The immunogenic composition has a wide application in the prevention of pneumonia, and is suitable for large-scale production in industry for its simple preparation method, low production cost, and short production cycle.

Abstract: Provided are a fusion protein and a construction method thereof. The fusion method consists of: a Haemophilus influenzae protein D and a Hin47 (Htra) protein. The fusion protein can serve as a protein vehicle for a Haemophilus influenzae polysaccharide-protein conjugate vaccine, thereby increasing immunogenicity of a polysaccharide antigen.

Abstract: A blower, comprising: a housing comprising an air inlet, a motor disposed in the housing, an axial fan configured to be driven to rotate about a fan axis and generate an air flow by the motor, a blowing tube configured to be coupled to the housing and comprising an air outlet, a duct configured to guide the air flow to the air outlet, the housing and the blowing tube comprises an air passage which comprises an upstream region between the inlet and the axial fan and a downstream region between the axial fan and the air outlet, the motor is disposed in the upstream region and the duct is disposed in the downstream region.

Abstract: A pouring metallic plug stick clamping mechanism for cast steel wheels. The mechanism includes: a cast iron frame, a movable adjusting groove, an internal fixing shaft, a first white ceramic ball, a second white ceramic ball, a third white ceramic ball, a retaining spring, a pressure regulating bolt, a control press button, a sector shaped panel, a metallic plug stick, and a blocking iron piece.

Abstract: The present invention provides a titanium ligand-modified black phosphorus and the preparation method and use thereof. The titanium ligand-modified black phosphorus is a complex of black phosphorus and a titanium ligand having a structure represented by formula (I): wherein in the formula (I), R1 comprises C1-C6 alkyl, or phenyl optionally further substituted with 0 to 5 groups each independently selected from halogen atom, C1-C6 alkyl, nitro, hydroxy, amino or C1-C3 alkoxy; the C1-C6 alkyl or C1-C3 alkoxy is optionally further substituted with 0 to 3 groups each independently selected from halogen atom, nitro, hydroxy, amino, methyl, ethyl or n-propyl. The titanium ligand-modified black phosphorus of the present invention is not likely oxidized without changing inherent properties of the black phosphorus, and the antioxidant capacity is greatly enhanced.

Abstract: A method for enhancing immunogenicity of an epitope peptide of an HPV antigen, the method including: assembling a gene of an HPV antigen into a gene of HBc, exogenously expressing a resulting assembled gene to acquire a fusion protein, allowing the fusion protein to automatically assemble to form a virus-like particle including the HPV antigen on a surface of the virus-like particle, to obtain HBc-HPV virus-like particle. A virus-like particle capable of expressing the antigen peptide of HPV acquired by the method. The virus-like particle includes a HBc-L2 fusion protein. A genome for encoding the HBc-L2 fusion protein is represented by SEQ ID NO. 3. A method for preparing an HPV vaccine includes using the virus-like particle.

Abstract: The present invention relates to a suction or blower device, comprising a housing having an air inlet and an air outlet, a motor accommodated in the housing, a fan driven by the motor, and a plurality of blowing accessories adapted to be connected to the air outlet respectively when the suction or blower device works. These different blowing accessories can perform various cleaning functions, thus meeting the cleaning demands in different places or in a specific area.

Abstract: A nutritional composition comprising by weight: between 0.05 and 30% selenium-enriched yeast; between 1 and 90% yeast beta-glucan; between 0.5 and 60% Vitamin C; and between 0.5 and 40% Vitamin E. The composition features anti-oxidative, anti-aging, cancer-preventing, and immune-stimulating properties.

Abstract: A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 ?m; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 ?m; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, ?-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.

Abstract: The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable Saccharomyces cerevisiae, acid protease, phytase, cellulose, ?-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.