Abstract: The disclosure relates to modified orthopoxvirus vectors, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified orthopoxvirus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences and safety. The viruses we have discovered are also amenable to large scale manufacturing protocols.

Abstract: Described herein are fixed doses and dosing regimens for long-acting insulin receptor agonists suitable for once-weekly dosing, such as weekly basal insulin-Fc (BIF).

Abstract: The disclosure relates to modified orthopoxvirus vectors, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified orthopoxvirus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences and safety. The viruses we have discovered are also amenable to large scale manufacturing protocols.

Abstract: Splitting of biological samples is provided by flowing the samples through a flow splitter where the sample strikes a stationary blade and is split into two pieces that end up in separate output channels. Samples can be single cells or multi-cellular samples. The split ratio of the pieces can be 50:50 or it can be other values as determined by design. To first order, the split ratio of the pieces is the same as the split ratio of the fluid flows in the output channels.

Abstract: An air purifying device including means for reducing air resistance between the air filtering elements and the target locations for the purified air. Reduced air resistance lowers the energy consumed by the device, thereby reducing its size and weight as well as improving ease of integration with a variety of head gear.

Abstract: The disclosure relates to modified vaccinia virus vectors derived from the Copenhagen strain of vaccinia virus, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified Copenhagen-derived vaccinia virus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences, amenability for large scale manufacturing, and safety.

Abstract: A method of determining outdoor characteristics of a photochromic optical article includes: determining environmental conditions for an area; positioning the optical article to face a first direction; determining a first incident irradiance on the optical article; determining a first surface temperature and first spectrum of the optical article; rotating the optical article to face a second direction; determining a second surface temperature and Full Characterization of Lens second spectrum of the optical article; determining a second incident irradiance on the optical article; and generating a prediction model of spectral transmission of the optical article. Further using environmental and climate conditions and to select a photochromic article most appropriate for an area.

Abstract: The disclosure relates to modified orthopoxvirus vectors, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified orthopoxvirus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences, amenability for large scale manufacturing, and safety.

Abstract: Heating, ventilation and air conditioning (HVAC) systems and methods of use are described. The HVAC system includes at least one internal surface temperature sensor configured to provide an interior wall surface temperature measurement. The internal surface temperature sensor is positioned on an interior surface of an external wall of the residential house. The HVAC system also includes a control system in communication over a network with the internal surface temperature sensor. The control system is configured to obtain the interior wall surface temperature measurement and apply a home heat transfer model to determine a predicted space air temperature. The home heat transfer model is based on thermal dynamics of space air and building structure using the interior wall surface temperature measurement.

Abstract: Compositions and methods for amplifying RNA by replication using transcription polymerases are disclosed. Such replicated RNAs can be used in various applications such as RNAi therapeutics, diagnostic probes, RNA sequencing, directed evolution of RNA aptamers without intermediate conversion to DNA, and RNA vaccines. The transcription polymerases comprise T7 bacteriophage RNA polymerase.

Abstract: A microscale biological tissue cutting device is made of a horizontal array of identically shaped polygonal through holes between vertically-oriented blades which form the sides of the polygonal through holes. Each of the through holes has a width less than 1 mm. The blades are joined at vertices of the polygonal through holes and have vertical peaks at the vertices. The vertical peaks have heights in the range 1-200 ?m above a lowest height of a cutting edge of the blades. The blades may be made of a material such as silicon, glass, plastic, resin, or metal.

Abstract: The disclosure relates to modified orthopoxvirus vectors, as well as methods of using the same for the treatment of various cancers. The disclosure provides modified orthopoxvirus vectors that exhibit various beneficial therapeutic activities, including enhanced oncolytic activity, spread of infection, immune evasion, tumor persistence, capacity for incorporation of exogenous DNA sequences, amenability for large scale manufacturing, and safety.

Abstract: Devices, methods, and kits are provided for allergy testing based on basophil activation in a blood sample. Susceptibility of an individual to an allergic reaction to an allergen is detected by collecting a blood sample from the individual and assaying for activation of basophils in response to stimulation with an allergen. In particular, a microfluidic device is provided for automating the assay for detecting basophil activation as an indication of the susceptibility of an individual to an allergic reaction as well as kits containing such a device and diagnostic methods for using such a device for allergy testing. Additionally, such a microfluidic device can be adapted for multiplexed detection of allergic responses to multiple allergens by performing assays in parallel to detect the basophil responses to each allergen.

Abstract: Events in an interactive application (e.g., a location-based parallel reality game) are triggered by a user's real-world activity meeting one or more criteria. A background process executing on the user's client device periodically extracts activity data from an activity monitoring application (e.g., a fitness application) and provides it to a server hosting the interactive application. The server determines whether received activity data triggers an event and, if so, sends a notification of the event to the client device.

Abstract: Events in an interactive application (e.g., a location-based parallel reality game) are triggered by a user's real-world activity meeting one or more criteria. A background process executing on the user's client device periodically extracts activity data from an activity monitoring application (e.g., a fitness application) and provides it to a server hosting the interactive application. The server determines whether received activity data triggers an event and, if so, sends a notification of the event to the client device.