Abstract: A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcin and an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. A reagent and a kit therefor are provided. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample, and a reagent and a kit therefor are also provided. A monoclonal antibody and a polyclonal antibody are used for the assay. A process for producing these antibodies, and utilization of these antibodies is described.

Abstract: Human IgG1 monoclonal antibodies are produced by hybridoma ATCC HB10074 and bind to gp120 of HIV.

Abstract: A monoclonal antibody for human acid glutathion S-transferase. This monoclonal antibody can be utilized as a diagnostic for cancers such as cancer of liver, gastric cancer, and cancer of the blood.

Abstract: The invention is directed to the human hybridoma designated MCA 86 and having A.T.C.C. Accession No. HB 9669 and human monoclonal antibodies produced by hybridoma MCA 86. Human monoclonal antibodies produced by hybridoma MCA 86 immunologically binds to both gp41 and gp120 envelope glycoproteins of Human Immunodeficiency Virus (HIV). These monoclonal antibodies are useful in the diagnosis of HIV infection.

Abstract: P. aeruginosa E87Ag antigen comprising polysaccharide fraction with molecular weight of about 27,000 which is contained in lipopolysaccharide of P. aeruginosa; anti-P. aeruginosa human or mouse monoclonal antibody which recognizes said E87Ag antigen; and mouse-human or mouse-mouse hybridoma which produces said monoclonal antibody.The monoclonal antibody can be used for making diagnosis and therapy of infections with P. aeruginosa.

Abstract: Human monoclonal antibodies against cytomegalovirus. These antibodies can be advantageously used as a diagnostic, or preventive and remedy for cytomegalovirus infectious diseases.

Abstract: Mouse-human hybridoma, which produces monoclonal antivirus-human antibody reactive to herpes simplex virus, varicella zoster virus, and cytomegalo virus, obtained by allowing a human antibody cell, which is sensitized to virus or protein or glycoprotein arising from virus, to fuse with a mouse myeloma cell in the presence of mitogen in vitro.

Abstract: Antitumor protein hybrid, composed of a moiety which is substantially the fragment Fab of an antitumor immunoglobulin and a moiety which is substantially the fragment A of a diphtheria toxin, which is expressed by the following formula (I):Fab--S.sub.1 --(X).sub.n --S.sub.2 --FA).sub.m (I)(where Fab indicates a moiety which is substantially the fragment Fab of an antitumor immunoglobulin; FA indicates a moiety which is substantially the fragment A of a diphtheria toxin; X indicates a divalent organic radical; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising from the disulfide bond (--S--S-- bond) in an immunoglobulin and S.sub.2 a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for 0 to 1 and m stands for an integer of 1 to 5). This antitumor protein hybrid has remarkable and specific cytotoxicity against tumor cells, e.g., in mice.

Abstract: Antitumor protein hybrid, composed of a moiety which is substantially the fragment Fab of an antitumor immunogloblin and a moiety which is the subunit A of ricin, which is expressed by the following formula (I):Fab-S.sub.1 --(X).sub.n --S.sub.2 --RA).sub.m . . . (I)(where Fab indicates a moiety which is substantially the fragment Fab of an antitumor immunogloblin; RA indicates a moiety which is the subunit A of ricin; X indicates a divalent organic radical; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising from the disulfide bond (--S--S-- bond) in an immunoglobulin and S.sub.2 a sulfur atom arising from the disulfide bond in ricin; n stands for 0 or 1 and m stands for an integer of 1 to 5). This antitumor protein hybrid has remarkable and specific cytotoxicity against tumor cells.

Abstract: Antitumor protein hybrid, having a moiety consisting of the antitumor immunoglobulin and a moiety substantially consisting of the fragment A of a diphtheria toxin, which is expressed by the following formula (I):Ab(NHCO--X--S.sub.1 --S.sub.2 --FA).sub.n (I)(where Ab indicates a moiety consisting of the antitumor immunoglobulin and FA indicates a moiety substantially consisting of the fragment A of a diphtheria toxin; X is an alkylene group or phenylene group which either has or does not have a branching of 1 to 5 carbon atoms. N in the amido bond is a nitrogen atom arising from the amino group in the antitumor immunoglobulin; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.2 indicating a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for an integer of 1 to 5).This antitumor protein hybrid has remarkable and specific citotoxicity against tumor cells.

Abstract: A protein hybrid having cytotoxicity obtained by covalently bonding an immunoglobulin or its fragment, which is capable of binding selectively to an antigen possessed by a cell to be destroyed, to a protein, which is obtained from Momordica charantia and has an activity to terminate protein synthesis. This protein hybrid displays remarkable cytotoxicity against targel cells.

Abstract: A cytotoxic protein hybrid obtained by covalently bonding an immunoglobulin or its fragment, which is capable of linking selectively with an antigen possessed by a cell to be destroyed, to a protein, which is obtained from Phytolacca americana and has an activity to terminate protein synthesis. This protein hybrid displays remarkable cytotoxicity against target cells.

Abstract: Antitumor protein hybrid, composed of a moiety which is substantially the fragment Fab of an anti-.alpha.-fetoprotein antibody and a moiety which is substantially the fragment A of a diphtheria toxin, which is expressed by the following formula (I):Fab--(S.sub.1 --(X).sub.n --S.sub.2 --FA).sub.m (I)(where Fab indicates a moiety which is substantially the fragment Fab of an anti-.alpha.-fetoprotein antibody; FA indicates a moiety which is substantially the fragment A of diphtheria toxin; X indicates a divalent organic radical; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising from the disulfide bond (--S--S--bond) in an anti-.alpha.-fetoprotein antibody and S.sub.2 a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for 0 or 1 and m stands for an integer of 1 to 5). This antitumor protein hybrid has remarkable and specific citotoxicity against tumor cells.

Abstract: IgM derivatives into which acyl groups expressed by the following formula (I) are introduced to make their anticomplement activity weak by acylating the free amino groups existing in IgM up to 1-90% (acylation percentage)--COR (I)where R indicates an alkyl group having 1 to 4 carbon atoms, or a carboxylated alkyl group expressed by --(CH.sub.2)mCOOH (m is 2 or 3) or --CH.dbd.CHCOOH.These IgM derivatives can be used for making immunoglobulin preparations for intravenous injection use.

Abstract: Antitumor protein hybrid, composed of a moiety which is substantially the fragment Fab of an antitumor immunogloblin and a moiety which is the subunit A of ricin, which is expressed by the following formula (I):Fab(S.sub.1 -(X).sub.n -S.sub.2 -RA).sub.m (I)(where Fab indicates a moiety which is substantially the fragment Fab of an antitumor immunogloblin; RA indicates a moiety which is the subunit A of ricin; X indicates a divalent organic radical; S.sub.1 and S.sub.2 are both sulfur atoms, S.sub.1 indicating a sulfur atom arising from the disulfide bond (--S--S-- bond) in an immunoglobulin and S.sub.2 a sulfur atom arising from the disulfide bond in ricin; n stands for 0 or 1 and m stands for an integer of 1 to 5). This antitumor protein hybrid has remarkable and specific cytotoxicity against tumor cells.

Abstract: Novel immunoglobulin derivatives which are characterized in that in which the interchain disulfide bonds of immunoglobulin are predominantly cleaved, on the average 3 to 5 of the interchain disulfide bonds or the inter- and intra-chain disulfide bonds being cleaved, and so produced sulfur atoms (S-) are S-sulfonated (--S-SO.sub.3). The above immunoglobulin derivatives are prepared by reacting native immunoglobulin withA. a compound capable of forming tetrathionate ion, andB. a compound capable of forming in water, sulfite ion whereby cleaving on the average 3 to 5 inter-chain disulfide bonds, or inter- and intra-chain disulfide bonds, of the native immunoglobulin, and S-sulfonating (S-SO.sub.3) the so formed sulfur atoms. This invention also provides a water-soluble composition for injection containing said immunoglobulin derivative having the titre of anti-diphtheria of at least 1.0 I.U./ml at its concentration of 10.