Patents by Inventor Yee LEUNG

Yee LEUNG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170247402
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Application
    Filed: December 12, 2016
    Publication date: August 31, 2017
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Hye Eun Hoover
  • Publication number: 20170177488
    Abstract: A method, system, and computer-readable medium for evicting cache lines that includes determining that a first cache line is to be evicted from a first Last Level Cache (LLC) partition of a partitioned LLC, and sending, based on the determination, a first notification to a second LLC partition of the partitioned LLC. The method may also include receiving, in response to the first notification, an available indication indicating that the second LLC partition is available as a designated victim cache partition; performing a selection of the second LLC partition as the designated victim cache partition; and evicting the first cache line to the second LLC partition based on the selection.
    Type: Application
    Filed: December 22, 2015
    Publication date: June 22, 2017
    Inventors: Serena Wing Yee Leung, Ramaswamy Sivaramakrishnan, Sumti Jairath
  • Patent number: 9682970
    Abstract: The invention provides compounds, including fluorescent nucleic acid dyes, and methods for use including nucleic acid detection, nucleic acid amplification reactions, and high-resolution melt curve analysis. Further provided are kits, instruments and systems comprising compounds of the invention or adapted for use with compounds of the invention or other nucleic acid dyes.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: June 20, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Chingying Cheung, Wai-Yee Leung, Sarah Lynn Windler, Lori M. Roberts
  • Patent number: 9670476
    Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: June 6, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Hui Cen, Wai-Yee Leung
  • Patent number: 9670363
    Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
    Type: Grant
    Filed: April 9, 2014
    Date of Patent: June 6, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Lori Michelle Roberts
  • Patent number: 9579402
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: February 28, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Hye Eun Hoover
  • Patent number: 9580749
    Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: February 28, 2017
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Xing Xin
  • Publication number: 20170037248
    Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.
    Type: Application
    Filed: October 21, 2016
    Publication date: February 9, 2017
    Inventors: Robert Batchelor, Yue GE, Iain JOHNSON, Wai-Yee LEUNG, Jixiang LIU, Brian PATCH, Peter SMALLEY, Thomas STEINBERG, Kyle GEE
  • Publication number: 20160356681
    Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.
    Type: Application
    Filed: August 16, 2016
    Publication date: December 8, 2016
    Inventors: Wai-Yee LEUNG, Ching-Ying CHEUNG, Stephen YUE
  • Patent number: 9513284
    Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.
    Type: Grant
    Filed: November 14, 2013
    Date of Patent: December 6, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Richard Haugland, Wai-Yee Leung, Jixiang Liu
  • Patent number: 9423323
    Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: August 23, 2016
    Assignee: Life Technologies Corporation
    Inventors: Wai-Yee Leung, Ching-Ying Cheung, Stephen Yue
  • Publication number: 20160237421
    Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.
    Type: Application
    Filed: October 10, 2013
    Publication date: August 18, 2016
    Applicant: Biotium, Inc.
    Inventors: Fei Mao, Hui Cen, Wai-Yee Leung
  • Publication number: 20160033520
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Application
    Filed: June 8, 2015
    Publication date: February 4, 2016
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Hye Eun Hoover
  • Publication number: 20160025713
    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.
    Type: Application
    Filed: October 6, 2015
    Publication date: January 28, 2016
    Inventors: Kyle Gee, Courtenay Hart-Kerndt, Wai-Yee Leung, Wayne Patton, Aleksey Rukavishnikov, Richard Haugland, Zhenjun Diwu
  • Publication number: 20160024067
    Abstract: The invention provides compounds, including fluorescent nucleic acid dyes, and methods for use including nucleic acid detection, nucleic acid amplification reactions, and high-resolution melt curve analysis. Further provided are kits, instruments and systems comprising compounds of the invention or adapted for use with compounds of the invention or other nucleic acid dyes.
    Type: Application
    Filed: June 28, 2013
    Publication date: January 28, 2016
    Inventors: Fei Mao, Chingying Cheung, Wal-Yee Leung, Sarah Lynn Windler, Lori M. Roberts
  • Patent number: 9222127
    Abstract: The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.
    Type: Grant
    Filed: December 4, 2013
    Date of Patent: December 29, 2015
    Assignee: Allelogic Biosciences Corporation
    Inventors: Fei Mao, Xing Xin, Wai-Yee Leung
  • Patent number: 9212385
    Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: December 15, 2015
    Assignee: Life Technologies Corporation
    Inventors: Robert H. Batchelor, Yue Ge, Iain Johnson, Wai-Yee Leung, Jixiang Liu, Brian Patch, Peter Smalley, Thomas Steinberg, Kyle Gee
  • Patent number: 9193746
    Abstract: Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: November 24, 2015
    Assignee: BIOTIUM, INC.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Jie Yang
  • Patent number: 9164099
    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.
    Type: Grant
    Filed: May 8, 2013
    Date of Patent: October 20, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Gee, Aleksey Rukavishnikov, Courtenay Hart Kerndt, Richard Haugland, Wai-Yee Leung, Wayne Patton, Zhenjun Diwu
  • Publication number: 20150278092
    Abstract: A method and system for allocating data streams that includes receiving, at an allocator, a data stream. The data stream includes a memory address and data associated with the memory address. The method also includes examining, by the allocator, the data stream to make a determination that the data stream is a soft allocating data stream, and then sending, from the allocator based on the determination, a plurality of write probes to a plurality of caches, wherein each write probe of the plurality of write probes includes at least part of the memory address. Additionally, the method includes receiving, at the allocator in response to a write probe of the plurality of write probes, a cache line present acknowledgement from a cache of the plurality of caches, and directing, by the allocator in response to the cache line present acknowledgement, the data of the data stream to the cache.
    Type: Application
    Filed: March 31, 2015
    Publication date: October 1, 2015
    Inventors: David Richard Smentek, Kathirgamar Aingaran, Sumti Jairath, Manling Yang, Serena Wing Yee Leung, Paul N. Loewenstein