Abstract: The invention provides devices for improving assay protocol compliance, reproducibility, and sensitivity. In particular, the devices of the present invention are tailored to certain single-cell sequencing assays, including single-cell RNA sequencing assays that utilize pre-templated instant partitions (PIPs) templates, wherein such devices hold tubes stationary while performing sample preparation, provide reproducible volume removal from tubes, allow for improved magnetic separation of analytes from buffers, and provide reproducible centrifugation of tubes.

Abstract: The invention provides devices that generate monodisperse droplets from a bulk liquid. The devices include a shearing mechanism, a holder for a vessel containing a liquid, and an optical system that transmit light to, and detects light from, liquid in the vessel. The invention also provides methods of using such devices to produce monodisperse droplets.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: The present disclosure discloses a method for preparing a TEM sample, including: fixing a chip sample on a sample stage of a FIB system, where the chip sample includes a semiconductor substrate, a target pattern layer, and a top pattern layer. The chip sample has a cuboid structure and includes a first side and a second side opposite each other and composed of a length and a height. A first protective layer is formed on one of the first side and the second side. First-time FIB cutting is performed to remove the top pattern layer. A top surface of the target pattern layer is etched to form a FIB mark pattern. A second protective layer is formed on the top surface of the target pattern layer. Based on localization of the FIB mark pattern, second-time FIB cutting is performed to form the TEM sample.

Abstract: The invention provides methods and systems for drug screening by segregating single cells into droplets simultaneously and providing candidate compound to the single cells to measure cellular response. Methods of the present invention combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets simultaneously that encapsulate a single one of the template particles and single one of the single cells, provide to the single cells one or more candidate compounds, and measure a cellular response to the one or more candidate compounds.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: The disclosure provides methods for creating long oligonucleotide reagents that include barcodes and other element for sequencing library preparation, where the oligonucleotides are created by multiple tiers of ligation of shorter oligos. The disclosed methods work to extend short oligos that are attached to particles, thereby allowing one to create particles that carry large number of long sample preparation oligonucleotides without being required to synthesize those full-length molecules with a polymerase.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: The disclosure provides methods for creating long oligonucleotide reagents that include barcodes and other element for sequencing library preparation, where the oligonucleotides are created by multiple tiers of ligation of shorter oligos. The disclosed methods work to extend short oligos that are attached to particles, thereby allowing one to create particles that carry large number of long sample preparation oligonucleotides without being required to synthesize those full-length molecules with a polymerase.

Abstract: The invention provides methods for measuring quantities of mRNA transcripts present in a sample, where sequence information for each molecule is read from what is essentially a random start site within that molecule and in which a short binning index (e.g., about 3 bases) is added to the sequence information. The binning index is useful to resolve any bias arising with the use of the intrinsic sequences to uniquely identify and count the molecules.

Abstract: The invention discloses a power feedback control system and method of MGP system, including detecting the actual active power sent by the generator to the grid through the measurement and calculation module; making a difference between the measured active power and the given active power; calculating frequency regulation amount through the PI regulation module according to the difference, and taking it as feedback; calculating the frequency reference value of the converter in the control system; through the adjustment module adjusts finely the frequency of the converter and adjust the phase difference of frequency modulation to achieve controlling power output, so when the new energy predicts its output power based on power feedback control using MGP before connecting to grid, the output of the control system will not delay and affect the stability and reliability of the control system, ensuring the smooth introduction of new energy grid-connected methods.

Abstract: An anti-IL23 antibody fusion protein and uses thereof, the anti-IL23 antibody fusion protein containing anti IL23 antibodies and TAC polypeptides, and the IL23 antibodies specifically binding the human IL23 p19 subunit. The anti-IL23 antibody fusion protein can be used for the treatment or improvement of B cell disorders or autoimmune diseases.

Abstract: The invention discloses a method for MGP new energy grid-connected control, wherein, the synchronous motor is connected to a synchronous generator; the new energy module drives the synchronous motor to rotate through a frequency converter; the frequency converter controls the power transmission; and the synchronous motor drives the synchronous generator for grid connection. The present invention has the beneficial effects that: the control method and system provided by the invention can improve the stability and reliability of the grid.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.