Abstract: A mechanism for building decentralized computer applications that execute on a distributed computing system. The present technology works within a web browser, client application, or other software and provides access to decentralized computer applications through the browser. The present technology is non-custodial, wherein a public-private key pair, which represents user identity, is created on a client machine and then directly encrypted by a third-party platform without relying on one centralized computing system.

Abstract: A piezoelectric device comprises: a substrate (12) and a lead magnesium niobate-lead titanate (PMNPT) piezoelectric film on the substrate (12). The PMNPT film comprises: a thermal oxide layer (20) on the substrate (12); a first electrode above on the thermal oxide layer (20); a seed layer (26) above the first electrode; a lead magnesium niobate-lead titanate (PMNPT) piezoelectric layer (16) on the seed layer (26), and a second electrode on the PMNPT piezoelectric layer (16). The PMNPT film comprises a piezoelectric coefficient (d33) of greater than or equal to 200 pm/V.

Abstract: A multilayered rail guide vehicle system for use in three-dimensional planting in a plant factory and a control method therefor, the system includes an upper computer, cultivation shelves, traveling devices, lifting devices, and an electric control system. The traveling devices and the lifting devices are arranged in a body of a rail guide vehicle. The upper computer is in wireless communication with the electric control system. A nutrient solution tank is arranged on each of the cultivation shelves, and a plurality of cultivation positions are arranged on the nutrient solution tank. The electric control system is arranged in the body of the rail guide vehicle, so as to obtain an operation mode and target information after receiving a task instruction transmitted by the upper computer, select and switch a positioning mode, and drive the traveling devices and the lifting devices to perform traveling and lifting positioning according to operation procedures.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.

Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: The invention provides methods and systems for drug screening by segregating single cells into droplets simultaneously and providing candidate compound to the single cells to measure cellular response. Methods of the present invention combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets simultaneously that encapsulate a single one of the template particles and single one of the single cells, provide to the single cells one or more candidate compounds, and measure a cellular response to the one or more candidate compounds.

Abstract: The invention provides devices for improving assay protocol compliance, reproducibility, and sensitivity. In particular, the devices of the present invention are tailored to certain single-cell sequencing assays, including single-cell RNA sequencing assays that utilize pre-templated instant partitions (PIPs) templates, wherein such devices provide improved means of holding tubes stationary while performing sample preparation, provide reproducible volume removal from tubes, allow for improved magnetic separation of analytes from buffers, and provide reproducible centrifugation of tubes.

Abstract: Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

Abstract: The invention provides methods and systems for drug screening by segregating single cells into droplets simultaneously and providing candidate compound to the single cells to measure cellular response. Methods of the present invention combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets simultaneously that encapsulate a single one of the template particles and single one of the single cells, provide to the single cells one or more candidate compounds, and measure a cellular response to the one or more candidate compounds.

Abstract: The present invention includes compositions and methods for using and manufacturing hydrogel template particles with micropores and/or a nanoporous structure.

Abstract: This disclosure provides methods and systems for single-extracellular (EV), multi-omic analysis of target EVs without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target EV from a population of EVs in an encapsulation, derive a plurality of distinct mRNA molecules from the single target EV, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling, which enables classification of an EV in a sample.

Abstract: An inter-frame prediction method and apparatus, and related devices and a storage medium. The method comprises: determining a first motion vector and a second motion vector of a current block, wherein the first motion vector is a forward motion vector of the current block, and the second motion vector is a backward motion vector of the current block; on the basis of the first motion vector and the second motion vector, determining whether a set condition is satisfied, and obtaining a determination result; setting the value of a BDOF flag according to the determination result; and determining, on the basis of the value of the BDOF flag, an inter-frame prediction value of the current block or at least one sub-block in the current block.

Abstract: Methods to emulsify cells and/or mRNA with reverse transcriptase at a temperature such that the reverse transcriptase begins making cDNA during the emulsification

Abstract: The invention discloses a power feedback control system and method of MGP system, including detecting the actual active power delivered by the generator to the grid through the measurement and calculation module; making a difference between the measured active power and the given active power; calculating the frequency regulation amount through the PI regulation module according to the difference, and taking it as feedback; calculating the frequency reference value of the converter in the control system; and fine tuning the frequency of the converter through the PI regulation module; regulating the phase difference through frequency modulation; realizing the goal of controlling the power output, so that when predicting the output power of new energy before MGP is connected to the grid based on power feedback control, the output of the control system will not be delayed and the defects that affect the stability and reliability of the control system will not appear, to ensure the successful introduction of new

Abstract: The invention discloses a method for MGP new energy grid-connected control, wherein, the synchronous motor is connected to a synchronous generator; the new energy module drives the synchronous motor to rotate through a frequency converter; the frequency converter controls the power transmission; and the synchronous motor drives the synchronous generator for grid connection. The present invention has the beneficial effects that: the control method and system provided by the invention can improve the stability and reliability of the grid.