Abstract: Methods to emulsify cells and/or mRNA with reverse transcriptase at a temperature such that the reverse transcriptase begins making cDNA during the emulsification

Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.

Abstract: The invention provides methods and systems for drug screening by segregating single cells into droplets simultaneously and providing candidate compound to the single cells to measure cellular response. Methods of the present invention combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets simultaneously that encapsulate a single one of the template particles and single one of the single cells, provide to the single cells one or more candidate compounds, and measure a cellular response to the one or more candidate compounds.

Abstract: This disclosure provides methods and systems for amplifying small amounts of nucleic acids. Methods include performing amplification reactions in an emulsion format to isolate and clonally amplify discrete populations of nucleic acid molecules inside droplets. In particular, methods and systems of the invention generate an emulsion with particles that template the formation of droplets inside a tube and segregate nucleic acid molecules therein such that each droplet contains an individual nucleic acid molecule. The nucleic acid molecules are amplified inside the droplets.

Abstract: A resource allocation method is provided, the method includes: receives a resource scheduling request by a first side unit (RSU) sent from a first vehicle to everything (V2X) communication group which comprises vehicles in a same driving direction; allocating communication resources for the first V2X communication group. A road side unit is also provided. By utilizing embodiments of the present disclosure, rationality of resource allocation can be improved, qualities of vehicle communication links and channels in the communication group is stable and a performance of vehicle networking can be fully realized.

Abstract: The present invention discloses a catalyst for preparing pyridine base from syngas. The catalyst includes a carrier, an active component, a first auxiliary and a second auxiliary. The carrier is molecular sieves. The active component is Rh. The first auxiliary is one or more of Mn, Fe, Na and La. The second auxiliary is one or more of Zn, Co, Cr, Bi and Cu. The active component Rh is 0.5-3% of a mass of the carrier. The first auxiliary is 0.05-5% of the mass of the carrier. The second auxiliary is 0.5-15% of the mass of the carrier. The present invention further discloses application of the catalyst to preparation of pyridine base by catalyzing syngas, where the syngas and an ammonia donor are used as reaction raw materials for reaction to generate pyridine base products.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: Disclosed is a method for preparing 2,2?-dipyridine and derivatives thereof. The method includes: using pyridine represented by formula I or a derivative thereof as a raw material to generate 2,2?-dipyridine represented by formula II by performing dehydrogenative coupling under the action of a supported catalyst in the presence of additives, where R is H, C1-C2 alkyl, Cl, or Br. The method of the present invention features wide adaptability to raw materials, high atomic utilization rate, high catalyst activity, long service life, and fewer by-products.

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

Abstract: A method for preparing 1,1?-ethylene-2,2?-bipyridinium dichloride includes mixing an aqueous diquat dibromide solution with hydrochloric acid, adding dropwise to a mixture of an organic solvent, a bromine absorbent, and an oxidant, and reacting to produce 1,1?-ethylene-2,2?-bipyridinium dichloride.

Abstract: Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

Abstract: Disclosed is a method for preparing 2,2?-dipyridine and derivatives thereof. The method includes: using pyridine represented by formula I or a derivative thereof as a raw material to generate 2,2?-dipyridine represented by formula II by performing dehydrogenative coupling under the action of a supported catalyst in the presence of additives, where R is H, C1-C2 alkyl, Cl, or Br. The method of the present invention features wide adaptability to raw materials, high atomic utilization rate, high catalyst activity, long service life, and fewer by-products.

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

Abstract: A method for preparing 1,1?-ethylene-2,2?-bipyridinium dichloride includes mixing an aqueous diquat dibromide solution with hydrochloric acid, adding dropwise to a mixture of an organic solvent, a bromine absorbent, and an oxidant, and reacting to produce 1,1?-ethylene-2,2?-bipyridinium dichloride.

Abstract: The present invention discloses a connection management method in device-to-device (D2D) relay communication, a terminal and a base station. The connection management method in D2D relay communication includes: determining whether a relay terminal for performing the D2D relay communication is selected when an Radio Resource Control (RRC) connection between a remote terminal in a network coverage and a base station is not released; when the relay terminal is determined to be selected, determining whether the RRC connection with the base station can be released according to a resource configuration mode used by the remote terminal when performing a D2D communication. By utilizing the connection management method, problems of state transition and connection management of the terminal in the D2D relay communication can be solved.

Abstract: Non-fault disturbance-based method and system for measuring short-circuit capacity of a power grid on site including: connecting or disconnecting a reactive compensation device to or from a power grid point of common coupling, to generate a disturbance on a power grid; obtaining total active power and total reactive power of a load of the point of common coupling before the disturbance; determining a vector difference between a voltage of the power grid point of common coupling before the disturbance and a voltage of the power grid point of common coupling after the disturbance; obtaining a voltage effective value of the power grid point of common coupling before the disturbance; obtaining a capacity of the reactive compensation device; and determining a short-circuit capacity of the point of common coupling according to total active power, total reactive power, vector difference between voltages, voltage effective value, and capacity of the reactive compensation device.

Abstract: The present disclosure provides a method for broadcasting system information. The method includes: obtaining preset global system information, the global system information including pre-divided basic system information and terminal-related system information; broadcasting the basic system information on a broadcast channel; receiving a message uploaded by User Equipment (UE) through an uplink channel, and extracting a system information requirement message included in the message; determining target system information in the terminal-related system information according to an indication of the system information requirement message. By adopting the present disclosure, there is no need to send all system information to a terminal at one time, thereby occupying less capacity resources of a base station broadcast channel and improving resource utilization of the broadcast channel.

Abstract: An embodiment of the disclosure provides a resource partition aggregating method and a device, the method includes acquiring a resource utilization ratio of each resource partition in a first signal unit. When there is a resource utilization ratio of each resource partition of at least two adjacent resource partitions in the first signal unit is less than a first preset threshold, whether a zone index used after aggregating the at least two adjacent resource partitions causes a communication interference with zone indexes used by resource partitions in an adjacent second signal unit is determined. If no communication interference is caused, determining area distribution parameters of the aggregated at least two adjacent resource partitions. A broadcast message carrying the area distribution parameters to a vehicle terminal is sent. By using the embodiment of the present disclosure, a resource utilization ratio can be improved.

Abstract: An RRM measuring method of an LTE system in an unlicensed frequency band, a system, a terminal and a base station are provided. The method includes: receiving a downlink signal sent by each base station in at least one base station to determine a busy/idle status of an uplink channel and/or a downlink channel of each base station; calculating a value of RSRQ of each base station, when the uplink channel and/or the downlink channel of each base station is in a busy state or in an idle state; reporting the values of RSRQ to a service base station. The present disclosure measures the values of RSRQ according to the channel statuses of the base station, thus the serving base station determines the service performance of a neighboring base station to select a more appropriate auxiliary cell base station or a target base station when switching.