Abstract: Exosomes are a small type of extracellular vesicles containing nucleic acids, lipids, and proteins that are implicated in tumorigenesis, metastasis, and cardiac regeneration, and therefore serve as potentially useful biomarkers from fluids or as vehicles for drug delivery. Global bottom-up mass spectrometry-based proteomics has been previously used to profile exosome cargo for diagnostic purposes. However, the current protocols for MS analysis of extracellular vesicles and exosome proteomics are challenging due to labor-intensive sample preparation, including lengthy digestion times and removal of MS incompatible reagents, and the need for high sensitivity. To address these challenges, the present invention provides a novel, high-throughput strategy for extracellular vesicle analysis and exosome proteomics using a photo-cleavable, anionic surfactant, preferably 4-hexylphenylazosulfonate (Azo).

Abstract: An information pushing method, including receiving an information pushing request of an offline service provider; acquiring target information to be pushed and related information about the offline service provider based on the information pushing request; and based on determining that information pushing is to be performed, pushing information to a user such that the pushed information matches with user interests, based on the target information, the related information about the offline service provider, a service area of the user, and an interest probability distribution of the user in the service area, wherein the service area and the interest probability distribution are obtained based on offline visit record information reported by the user, and wherein the offline visit record information is generated based on service attribute information collected by a mobile terminal device of the user at offline service providers visited by the user.

Abstract: The present invention provides nonionic cleavable surfactants (NCS), specifically n-Decyl-disulfide-?-D-maltoside (DSSM), suitable for MS-based proteomics and analysis. These surfactants are designed to mimic the properties of a commonly used surfactant in structural biology, n-dodecyl-?-d-maltoside (DDM), but contain a disulfide bond that allows for facile cleavage and surfactant removal before analysis. DSSM and other NCS are compatible with native mass spectrometry, top-down and bottom-up proteomics, ESI-MS and other analytical techniques, and reduce signal suppression typically observed with other surfactants. DSSM and other NCS provide versatile surfactants that can facilitate protein sample preparation under non-denaturing conditions for a myriad of proteomic and structural biology applications and act as a general replacement for DDM.

Abstract: tion of heme oxygenase 1 (HO-1), in particular porphyrin, chlorin, bacteriochlorin or isobacteriochlorin compounds of formula I as defined herein having a tetrapyrrole or reduced tetrapyrrole backbone and a fluorophore. Such compounds can be used in the detection of HO-1 in vivo, ex vivo and in vitro, and can also be used in methods of diagnosis and as research reagents.

Abstract: An information pushing method, including receiving an information pushing request of an offline service provider; acquiring target information to be pushed and related information about the offline service provider based on the information pushing request; and based on determining that information pushing is to be performed, pushing information to a user such that the pushed information matches with user interests, based on the target information, the related information about the offline service provider, a service area of the user, and an interest probability distribution of the user in the service area, wherein the service area and the interest probability distribution are obtained based on offline visit record information reported by the user, and wherein the offline visit record information is generated based on service attribute information collected by a mobile terminal device of the user at offline service providers visited by the user.

Abstract: The present invention provides photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded upon UV irradiation, for top-down and bottom-up proteomics. These surfactants can effectively solubilize proteins and peptide fragments with performance comparable to sodium dodecyl sulfate (SDS) and are compatible with mass spectrometry analysis of the solubilized proteins and peptide fragments. Top-down proteomic studies using the present photo-cleavable anionic surfactants has allowed the detection of 100-fold more unique proteoforms as compared to controls and has enabled the solubilization of membrane proteins for comprehensive characterization of protein post-translational modifications.

Abstract: Exosomes are a small type of extracellular vesicles containing nucleic acids, lipids, and proteins that are implicated in tumorigenesis, metastasis, and cardiac regeneration, and therefore serve as potentially useful biomarkers from fluids or as vehicles for drug delivery. Global bottom-up mass spectrometry-based proteomics has been previously used to profile exosome cargo for diagnostic purposes. However, the current protocols for MS analysis of extracellular vesicles and exosome proteomics are challenging due to labor-intensive sample preparation, including lengthy digestion times and removal of MS incompatible reagents, and the need for high sensitivity. To address these challenges, the present invention provides a novel, high-throughput strategy for extracellular vesicle analysis and exosome proteomics using a photo-cleavable, anionic surfactant, preferably 4-hexylphenylazosulfonate (Azo).

Abstract: This invention provides mass spectrometry (MS) compatible nanomaterials for the selective capture and enrichment of low abundance proteins as well as MS analysis of different proteoforms of proteins, particularly cardiac proteins and different proteoforms of cardiac troponin I (cTnI) arising from post-translational modifications and sequence variations. The surface of superparamagnetic nanoparticles is functionalized with probe molecules that specifically bind to the desired protein. In an embodiment, the nanoparticles are functionalized with probe molecules having high affinity and selectivity for cTnI within the human cardiac troponin complex. This allows for MS-analysis and characterization of cTnI proteoforms from human heart tissue lysates and human blood or serum samples, and provides an accurate assay for detection of cTnI with molecular details.

Abstract: The present invention provides photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded upon UV irradiation, for top-down and bottom-up proteomics. These surfactants can effectively solubilize proteins and peptide fragments with performance comparable to sodium dodecyl sulfate (SDS) and are compatible with mass spectrometry analysis of the solubilized proteins and peptide fragments. Top-down proteomic studies using the present photo-cleavable anionic surfactants has allowed the detection of 100-fold more unique proteoforms as compared to controls and has enabled the solubilization of membrane proteins for comprehensive characterization of protein post-translational modifications.

Abstract: A method for displaying a video poster based on artificial intelligence is provided. The method includes obtaining a target video and at least one poster according to a user input, calculating a matching degree of an image description text of the at least one poster and a description text keyword of the target video, and sorting the at least one poster according to the matching degree in an order from high to low, to obtain a sorted poster sequence; performing element separation on one of the at least one poster with a highest matching degree in the poster sequence to obtain a main body element and a background element; and processing the main body element, the background element and title information of the target video according to requirements of a target scene to synthesize a new poster.

Abstract: The application discloses a method for managing food in a refrigerator, including the steps of recognizing the type and quantity of the food placed in the refrigerator according to an image shot by an external camera of the refrigerator; determining a position of the food placed in the refrigerator according to images shot by internal cameras of the refrigerator; generating an AR label for recording information of the food with regard to the food placed in the refrigerator; displaying the AR label of the inquired food after receiving a food query request from a user; wherein the information of the food at least includes the type, quantity and position of the food; and displaying the images shot by the internal cameras in real time after receiving an image viewing request from the user. With the adoption of the application, the food in the refrigerator can be managed more efficiently.

Abstract: A method for displaying a video poster based on artificial intelligence is provided. The method includes obtaining a target video and at least one poster according to a user input, calculating a matching degree of an image description text of the at least one poster and a description text keyword of the target video, and sorting the at least one poster according to the matching degree in an order from high to low, to obtain a sorted poster sequence; performing element separation on one of the at least one poster with a highest matching degree in the poster sequence to obtain a main body element and a background element; and processing the main body element, the background element and title information of the target video according to requirements of a target scene to synthesize a new poster.

Abstract: A fingerprint identification module and a terminal device are provided. The fingerprint identification module includes: a decorative ring, provided with a mounting opening; an encapsulation cover, arranged on the decorative ring to close a side of the mounting opening; a fingerprint chip, disposed in the mounting opening, a space is formed between an outer side surface of the fingerprint chip and an inner wall surface of the decorative ring; a protective film, disposed on a side of the fingerprint chip that is towards the encapsulation cover; and a flexible printed circuit (FPC) board, disposed inside the mounting opening, the protective film covers a first partial area of the FPC board, and a second partial area of the FPC board is exposed out of the protective film; the second partial area is an area of the FPC board which is different from the first partial area of the FPC board.

Abstract: The present invention provides photo-cleavable anionic surfactants, particularly 4-hexylphenylazosulfonate (Azo) and sodium 4-hexylphenylazosulfonate derivatives, which can be rapidly degraded upon UV irradiation, for top-down and bottom-up proteomics. These surfactants can effectively solubilize proteins and peptide fragments with performance comparable to sodium dodecyl sulfate (SDS) and are compatible with mass spectrometry analysis of the solubilized proteins and peptide fragments. Top-down proteomic studies using the present photo-cleavable anionic surfactants has allowed the detection of 100-fold more unique proteoforms as compared to controls and has enabled the solubilization of membrane proteins for comprehensive characterization of protein post-trans-modifications.

Abstract: The application discloses a method for managing food in a refrigerator, including the steps of recognizing the type and quantity of the food placed in the refrigerator according to an image shot by an external camera of the refrigerator; determining a position of the food placed in the refrigerator according to images shot by internal cameras of the refrigerator; generating an AR label for recording information of the food with regard to the food placed in the refrigerator; displaying the AR label of the inquired food after receiving a food query request from a user; wherein the information of the food at least includes the type, quantity and position of the food; and displaying the images shot by the internal cameras in real time after receiving an image viewing request from the user. With the adoption of the application, the food in the refrigerator can be managed more efficiently.

Abstract: In accordance with an aspect of the present disclosure, a method for encrypting/hiding or decrypting/unhiding a target object on a device is provided. The method comprises binding with a binding module; detecting an operation instruction for the target object; analyzing the detected operation instruction; outputting information to be confirmed for encrypting or hiding the target object if the detected operation instruction for the target object is a preset encryption instruction or a preset hiding instruction; and encrypting or hiding the target object after receiving a confirmation input.

Abstract: A fingerprint identification module and a terminal device are provided. The fingerprint identification module includes: a decorative ring, provided with a mounting opening; an encapsulation cover, arranged on the decorative ring to close a side of the mounting opening; a fingerprint chip, disposed in the mounting opening, a space is formed between an outer side surface of the fingerprint chip and an inner wall surface of the decorative ring; a protective film, disposed on a side of the fingerprint chip that is towards the encapsulation cover; and a flexible printed circuit (FPC) board, disposed inside the mounting opening, the protective film covers a first partial area of the FPC board, and a second partial area of the FPC board is exposed out of the protective film; the second partial area is an area of the FPC board which is different from the first partial area of the FPC board.

Abstract: This invention relates to an engineered leader-independent heterocyclase (also known as a cyclodehydratase) comprising a defined cyanobactin leader sequence which drives the efficient conversion of heterocyclisable amino acids, such as Ser, Thr and Cys, within a peptide substrate lacking a leader sequence into heterocycles produce a homogenous heterocycle-containing product. This may be useful in biotechnology and chemical synthesis.

Abstract: This invention relates to an engineered leader-independent heterocyclase (also known as a cyclodehydratase) comprising a defined cyanobactin leader sequence which drives the efficient conversion of heterocyclisable amino acids, such as Ser, Thr and Cys, within a peptide substrate lacking a leader sequence into heterocycles produce a homogenous heterocycle-containing product. This may be useful in biotechnology and chemical synthesis.

Abstract: In accordance with an aspect of the present disclosure, a method for encrypting/hiding or decrypting/unhiding a target object on a device is provided. The method comprises binding with a binding module; detecting an operation instruction for the target object; analyzing the detected operation instruction; outputting information to be confirmed for encrypting or hiding the target object if the detected operation instruction for the target object is a preset encryption instruction or a preset hiding instruction; and encrypting or hiding the target object after receiving a confirmation input.