Abstract: The present application provides a separator, a secondary battery, a battery module, a battery pack and an electrical apparatus. The separator may sequentially include a first region, a main region and a second region in a first direction. At least one surface of the first region and/or at least one surface of the second region may be provided with a fluorescent coating. The fluorescent coating may be used to identify whether the separator is folded or not.

Abstract: The present disclosure relates to the technical field of rapid detection of molecules, and specifically relates to a method for rapid fluorescent immunoassay (FIA) and chemiluminescent immunoassay (CLIA) based on electrokinetic acceleration. The method includes the following steps sequentially: S1. sample acceleration: applying an actuating signal to a chip on which a target molecule is dripped to obtain a chip binding to the target molecule, where the chip includes an electrode sheet and coating molecules is immobilized on the electrode sheet; and S2. secondary antibody acceleration: adding a secondary antibody for binding to the target molecule dropwise on the chip binding to the target molecule, and applying an actuating signal to the chip to obtain a chip binding to the secondary antibody. The method can effectively improve a rate of FIA and CLIA, and can speed up a detection process and meet the need for rapid point-of-care testing (POCT).

Abstract: A 5?Cap analog having a Cap2 structure and a preparation method therefor. The molecular formula of the 5? Cap analog having a Cap2 structure is selected from any one of m7G(5?)ppp(5?)(2?OMeA)p(2?OMeG), m7G(5?)ppp(5?)(2?OMeG)p(2?OMeG), etc. The 5?Cap analog having a Cap2 structure has higher synthesis efficiency, higher capping efficiency, lower immunogenicity, and higher protein translation efficiency.

Abstract: A parameter configuration method includes that a service layer of an embedded device that can be applied to a vehicle such as an intelligent vehicle, a connected vehicle, or a new energy vehicle directly obtains a hardware resource parameter of a hardware layer, and sends the hardware resource parameter to an AUTomotive Open System ARchitecture (AUTOSAR) development tool such that the AUTOSAR development tool generates a configuration file and an executable file.

Abstract: Disclosed herein are an mRNA targeting molecule comprising an N-acetylgalactosamine binding polypeptide and a preparation method therefor. A plasmid vector containing a DNA fragment formed by sequentially connecting a promoter, a target gene, a specific protease cleavage sequence, and a polynucleotide sequence encoding a GBD capable of binding to N-acetylgalactosamine, is transcribed to obtain an mRNA, which is connected to a DNA-puromycin linker under the action of a T4 ligase. The resulting connection product is subjected to protein translation, followed by cleavage using a specific protease to obtain an mRNA-puromycin-GBD complex, which then binds to a GBD protein sequence under the action of an N-acetylgalactosamine transferase to form an mRNA-puromycin-GBD-GalNAc complex, thereby modifying the mRNA with GalNAc, thus achieving the purpose of precise administration in a process of mRNA drug delivery and increasing the efficacy of the mRNA drug molecule.

Abstract: Disclosed are a display panel, a manufacturing method thereof and display equipment. The display panel comprises a display area, the display area comprises a camera area and a peripheral area surrounding the camera area, the camera area is provided with a transparent first touch control structure, and the first touch control structure is arranged between a substrate and an organic luminescent layer.

Abstract: The present invention provides a mannose-based mRNA targeted delivery system and use thereof. The mRNA can encode one or more target polypeptides and contains at least one mannose modification. The technical solution of the present invention modifies the mRNA molecule with a mannose, so that the mRNA can be directly and efficiently coupled with the mannose, and the targeted delivery of the mRNA is realized without the need for a carrier.

Abstract: A data transmission method includes receiving, by a user plane function entity, a data packet from a first terminal through an uplink path corresponding to the first terminal, where the data packet carries addressing information of a second terminal; determining, by the user plane function entity based on information about the uplink path corresponding to the first terminal and the addressing information of the second terminal, a downlink path corresponding to the second terminal; and sending, by the user plane function entity, the data packet to the second terminal through the downlink path corresponding to the second terminal.

Abstract: The present disclosure provides a power supply device and a high-power illumination system. The power supply device includes: a housing, where the housing includes a first bottom plate having a first surface on which a first heat sink is provided and a second surface on which a thermal conductive potting layer is provided; and a printed circuit board, where the printed circuit board is located in the housing, the printed circuit board includes a circuit board body and multiple electronic components arranged on the circuit board body, at least part of the multiple electronic components is arranged facing the second surface of the first bottom plate, and the electronic component of the at least part of the multiple electronic components is partially immersed in the thermal conductive potting layer to conduct heat dissipated by the electronic component to the first heat sink for natural heat dissipation of the electronic component.

Abstract: Provided are a method for preparing an mRNA-GalNAc targeting molecule, an in vivo delivery system therefor and use thereof. The mRNA-GalNAc targeting molecule comprises an mRNA molecule that is linked to PolyA modified with an N-acetylgalactosamine at 3?-end, wherein a sequence of the mRNA molecule comprises a 5? cap and a target gene sequence. By directly linking the mRNA molecule expressing the target gene to the polyA sequence coupled with GalNAc, an mRNA molecule with GalNAc at 3?-end is synthesized to realize the aim of targeted drug delivery in liver. The method is simpler and more reliable, and solves existing problems in coupling between mRNA and N-acetylgalactosamine.

Abstract: A data transmission method includes receiving, by a user plane function entity, a data packet from a first terminal through an uplink path corresponding to the first terminal, where the data packet carries addressing information of a second terminal; determining, by the user plane function entity based on information about the uplink path corresponding to the first terminal and the addressing information of the second terminal, a downlink path corresponding to the second terminal; and sending, by the user plane function entity, the data packet to the second terminal through the downlink path corresponding to the second terminal.

Abstract: Described herein are sliders and data storage devices that promote particle mobility to improve particle robustness. In some embodiments, a data storage device includes a recording medium and a slider. A surface of the slider air-bearing surface near the leading edge includes at least one roughening feature that causes the surface to be rougher than other surfaces of the slider to promote particle mobility. The roughening feature may include a regular or irregular pattern, and it may be created using a photoresist mask during the manufacturing process so that the surface is deliberately made rougher (e.g., has a higher friction coefficient) than the surface of a leading pad of the slider.

Abstract: Disclosed herein are sliders with deep holes, data storage devices including such sliders, and methods of manufacturing such sliders. The holes can be situated near the edges of the slider to improve the stability and/or damping of the slider. The holes may be created, for example, using ion milling. In some embodiments, a slider comprises a leading pad comprising a first medium-facing surface that includes at least a first hole and a second hole, a first side pad comprising a second medium-facing surface that includes at least a third hole, and a second side pad comprising a third medium-facing surface that includes at least a fourth hole. In some embodiments, a trailing pad of the slider comprises a fourth medium-facing surface that includes at least a fifth hole and a sixth hole.

Abstract: The present disclosure provides a power supply device and a high-power illumination system. The power supply device includes: a housing, where the housing includes a first bottom plate having a first surface on which a first heat sink is provided and a second surface on which a thermal conductive potting layer is provided; and a printed circuit board, where the printed circuit board is located in the housing, the printed circuit board includes a circuit board body and multiple electronic components arranged on the circuit board body, at least part of the multiple electronic components is arranged facing the second surface of the first bottom plate, and the electronic component of the at least part of the multiple electronic components is partially immersed in the thermal conductive potting layer to conduct heat dissipated by the electronic component to the first heat sink for natural heat dissipation of the electronic component.

Abstract: A data transmission method, a device, and a data transmission system implement local interaction between two terminals when the two terminals are served by different UPF entities. The method performed by a first user plane function entity includes receiving a data packet from a first terminal through an uplink path corresponding to the first terminal, where the data packet carries addressing information of a second terminal; determining path information of a second user plane function entity based on information about the uplink path corresponding to the first terminal and the addressing information of the second terminal; sending the data packet to the second user plane function entity based on the path information of the second user plane function entity; and sending the data packet to the second terminal through a downlink path corresponding to the second terminal.

Abstract: Provided are a method for large-scale synthesis of a long-chain RNA and a method for site-specific modification of the long-chain RNA. The method for large-scale synthesis of a long-chain RNA comprises: designing short RNA fragments and splint DNA fragments; ligating; capping; and removing the splint DNA fragments and other steps. A large number of short RNA fragments and different splint DNA fragments are chemically synthesized, and then the different short RNA fragments are ligated by a biological method so as to form a target long-chain RNA. The product long-chain RNA has a low mutation rate, a plurality of the short RNA fragments can be assembled in a single reaction, and the long-chain RNA can be synthesized at a high throughput so as to fulfill the large-scale production of the long-chain RNA. In addition, by chemical modification of the short RNA fragments, the site-specific modification of the long-chain RNA can be realized.

Abstract: A data transmission method, a device, and a data transmission system are provided to implement local interaction between two terminals when the two terminals are served by different UPF entities. The method performed by a first user plane function entity includes receiving a data packet from a first terminal through an uplink path corresponding to the first terminal, where the data packet carries addressing information of a second terminal; determining path information of a second user plane function entity based on information about the uplink path corresponding to the first terminal and the addressing information of the second terminal; sending the data packet to the second user plane function entity based on the path information of the second user plane function entity; and sending the data packet to the second terminal through a downlink path corresponding to the second terminal.

Abstract: Disclosed herein are an mRNA targeting molecule comprising an N-acetylgalactosamine binding polypeptide and a preparation method therefor. A plasmid vector containing a DNA fragment formed by sequentially connecting a promoter, a target gene, a specific protease cleavage sequence, and a polynulcleotide sequence encoding a GBD capable of binding to N-acetylgalactosamine, is transcribed to obtain an mRNA, which is connected to a DNA-puromycin linker under the action of a T4 ligase. The resulting connection product is subjected to protein translation, followed by cleavage using a specific protease to obtain an mRNA-puromycin-GBD complex, which then binds to a GBD protein sequence under the action of an N-acetylgalactosamine transferase to form an mRNA-puromycin-GBD-GalNAc complex, thereby modifying the mRNA with GalNAc, thus achieving the purpose of precise administration in a process of mRNA drug delivery and increasing the efficacy of the mRNA drug molecule.

Abstract: A display substrate and a display device are disclosed. The display substrate includes a display area and a frame area, and the display substrate includes a substrate, a plurality of sub-pixels on a side of the substrate, the plurality of sub-pixels are configured to emit light in the display area, an encapsulation layer on a side of the plurality of sub-pixels away from the substrate, and a retaining wall located in the frame area, the retaining wall is located between the encapsulation layer and the substrate and includes a first sub-retaining wall, a second sub-retaining wall, and a transition area between the first sub-retaining wall and the second sub-retaining wall, the retaining wall includes at least one layer provided in the same layer, the at least one layer provided in the same layer continuously extends across the first sub-retaining wall, the second sub-retaining wall and the transition area.