Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.

Abstract: A method for preparing an activated expanded lymphocyte stock solution includes co-culturing, in a serum-free medium, a lymphocyte activator with peripheral blood mononuclear cells obtained via autologous peripheral whole blood centrifugal extraction, and thus completing lymphocyte culture activation. The cultured activated lymphocytes are placed into a serum-free medium. Subculturing is performed to prepare lymphocyte activation culture. A serum-free medium having a volume which is 5 to 10 times of the lymphocyte activation culture is added to the lymphocyte activation culture. Expansion culture is performed, wherein the number of expansion culture generations is 1 to 5. A step of centrifuging, washing, and collecting the activated expanded lymphocytes is performed.

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.

Abstract: An earphone, including: a housing, a first speaker unit, a second speaker unit. The housing has a sound output hole. The first speaker unit and the second speaker unit are separately independently fastened in the housing. The first speaker unit produces a sound toward the sound output hole. The second speaker unit is located on a side that is of the first speaker unit and that is away from the sound output hole and is spaced apart from the first speaker unit. A sound production frequency of the second speaker unit is smaller than a sound production frequency of the first speaker unit. A cavity is enclosed by the first speaker unit, the second speaker unit, and an inner wall of the housing, the cavity serves as a front cavity of the second speaker unit, and the front cavity communicates with the sound output hole through a sound output channel.

Abstract: A main winch system of a rotary drilling rig includes a main action loop, a pilot control loop, and a feedback control loop. The main action loop includes an engine, a hydraulic pump, a main control valve, a balance valve, a main winch motor, and an oil tank. The pilot control loop includes a pilot handle, a solenoid valve I, a pressure relay, and the main control valve in the main action loop. The feedback control loop includes a solenoid valve II and the hydraulic pump and the main control valve in the main action loop. In response to a lifting action of the pilot handle for the main winch, under the control of a button, the solenoid valve II is energized continuously, and the solenoid valve I is energized and de-energized according to a given rule.

Abstract: A main winch system of a rotary drilling rig includes a main action loop, a pilot control loop, and a feedback control loop. The main action loop includes an engine, a hydraulic pump, a main control valve, a balance valve, a main winch motor, and an oil tank. The pilot control loop includes a pilot handle, a solenoid valve I, a pressure relay, and the main control valve in the main action loop. The feedback control loop includes a solenoid valve II and the hydraulic pump and the main control valve in the main action loop. In response to a lifting action of the pilot handle for the main winch, under the control of a button, the solenoid valve II is energized continuously, and the solenoid valve I is energized and de-energized according to a given rule.

Abstract: Provided are a method and device for video search, a terminal and a storage medium. The method includes: receiving a first event generated by triggering a first control in a video playback page; acquiring, in response to the first event, a current video image frame played in the video playback page when the first event is triggered; acquiring a first to-be-searched target positioned by a second control in the current video image frame and a first display position of the first to-be-searched target in the current video image frame, and displaying the second control on the first display position; and acquiring a first recommendation result corresponding to the first to-be-searched target, and displaying the first recommendation result in a search result page.

Abstract: A push-pull force control method for a horizontal directional drilling machine with the following steps is provided. According to a correspondence relationship between a motor displacement and a maximum push-pull force of a drilling machine, the motor displacement is first adjusted, so that the required push-pull force can be obtained through the adjustment of the subsequent steps. Then, an oil feeding pressure of the motor is controlled according to a relationship between the push-pull force and the working pressure difference of the motor. Finally, the push-pull force of the motor is controlled in real time according to the oil feeding pressure of the motor to be equal to the required push-pull force value.

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.

Abstract: Provided are a method and device for video search, a terminal and a storage medium. The method includes: receiving a first event generated by triggering a first control in a video playback page; acquiring, in response to the first event, a current video image frame played in the video playback page when the first event is triggered; acquiring a first to-be-searched target positioned by a second control in the current video image frame and a first display position of the first to-be-searched target in the current video image frame, and displaying the second control on the first display position; and acquiring a first recommendation result corresponding to the first to-be-searched target, and displaying the first recommendation result in a search result page.

Abstract: The present invention provides a leveling differential-pressure diversion peak-shaving water tank with a function of preventing stagnant water and stale water, which comprises a water storage and distribution tank, a water inlet pipeline, a water outlet pipeline and a front tank. The water inlet pipeline is arranged at the upper portion of the water storage and distribution tank and used for feeding water into the water storage and distribution tank. The water outlet pipeline is arranged at the lower portion of the water storage and distribution tank and comprises a main water outlet pipe and a plurality of water outlet branch pipes. One end of each water outlet branch pipe is connected with the main water outlet pipe. The front tank comprises a front tank water box, a front tank water inlet pipeline and a front tank water outlet pipeline. The present invention further provides a water supply pumping station.

Abstract: A safety device for hydrodynamic pressure regulating pipe network includes a gate valve, which comprises at least a valve body and a gate. A water inlet of the gate valve is used to be connected to a water inlet pipeline. A hydraulic driving device, which comprises a hydraulic cylinder, is also included. The hydraulic cylinder includes a cylinder body, a piston and a piston rod. The piston rod is connected to the gate. The lower part of the inner cavity of the cylinder body is connected to the water inlet pipeline through a gate opening power pipelines. The upper part of the inner cavity of the cylinder body is connected to a gate closing driving device, which is used to drive the gate and close the gate valve.

Abstract: A push-pull force control method for a horizontal directional drilling machine and a horizontal directional drilling machine are provided. The control method includes: adjusting a working displacement of a motor to enable a maximum push-pull force Fmax corresponding to the working displacement to be greater than a set push-pull force Ft; calculating a working pressure difference ?P of the motor according to the set push-pull force Ft; calculating a working pressure required by the motor according to the working pressure difference ?P and a collected first oil return back pressure of the motor; and adjusting an oil feeding pressure of the motor to enable the oil feeding pressure of the motor to be equal to the working pressure required by the motor. The method accurately and fast controls the push-pull force of the horizontal directional drilling machine.

Abstract: A safety device for hydrodynamic pressure regulating pipe network includes a gate valve, which comprises at least a valve body and a gate. A water inlet of the gate valve is used to be connected to a water inlet pipeline. A hydraulic driving device, which comprises a hydraulic cylinder, is also included. The hydraulic cylinder includes a cylinder body, a piston and a piston rod. The piston rod is connected to the gate. The lower part of the inner cavity of the cylinder body is connected to the water inlet pipeline through a gate opening power pipelines. The upper part of the inner cavity of the cylinder body is connected to a gate closing driving device, which is used to drive the gate and close the gate valve.

Abstract: The present invention belongs to the field of biomedicine. Specifically, the present invention relates to an improved T-cell therapy. More specifically, the present invention relates to enhancing the cancer treatment efficacy of therapeutic T cells (such as CAR-T or TCR-T cells) through stimulation with living cells expressing cancer-related antigens.

Abstract: The present invention provides a leveling differential-pressure diversion peak-shaving water tank with a function of preventing stagnant water and stale water, which comprises a water storage and distribution tank, a water inlet pipeline, a water outlet pipeline and a front tank. The water inlet pipeline is arranged at the upper portion of the water storage and distribution tank and used for feeding water into the water storage and distribution tank. The water outlet pipeline is arranged at the lower portion of the water storage and distribution tank and comprises a main water outlet pipe and a plurality of water outlet branch pipes. One end of each water outlet branch pipe is connected with the main water outlet pipe. The front tank comprises a front tank water box, a front tank water inlet pipeline and a front tank water outlet pipeline. The present invention further provides a water supply pumping station.

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab.

Abstract: The present application discloses variants of Pertuzumab. In particular, it discloses: an unpaired cysteine variant comprising Cys23/Cys88 unpaired cysteines in one or both variable light domains of Pertuzumab, an afucosylated variant of Pertuzumab, a low-molecular-weight-species (LMWS) of Pertuzumab, and a high-molecular-weight-species (HMWS) or Pertuzumab. The application further discloses the isolated variants, compositions, pharmaceutical compositions, and articles of manufacture comprising the variants, as well as methods of making and characterizing the variants and compositions thereof.