Abstract: The present invention provides polypeptides and polynucleotides involved in C1 assimilation in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: The present invention provides polypeptides and polynucleotides involved in central intermediates metabolism in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: The present invention provides polypeptides and polynucleotides involved in amino acid biosynthesis in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: The present invention provides polypeptides and polynucleotides involved in the stress response to environmental changes in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: Nucleoside 5′-phosphate ester is produced by culturing a bacterium belonging to the genus Escherichia having an ability to produce nucleoside 5′-phosphate ester, in which ushA gene and aphA gene do not function normally, in a medium to produce and accumulate nucleoside 5′-phosphate ester in the medium, and collecting the nucleoside 5′-phosphate ester from the medium.

Abstract: There are provided novel 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase and prephenate dehydratase/chorismate mutase and DNAs coding the enzymes derived from Methylophilus methylotrophus.

Abstract: The present invention relates to a process for producing 5′-inosinic acid or 5′-guanylic acid for use in seasonings or the like from inosine or guanosine or a precursor thereof using an adenosine triphosphate (ATP)-regenerating microorganism containing a DNA encoding a protein that has the activity of forming 5′-inosinic acid or 5′-guanylic acid from inosine or guanosine. Further, the present invention relates to a novel protein having the inosine-guanosine kinase activity, a gene encoding said protein, a recombinant DNA containing said gene, and a microorganism which is transformed with said recombinant DNA.

Abstract: The present invention provides polypeptides and polynucleotides involved in C1 assimilation in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: A protein which has a GMP synthetase activity, a DNA which codes for the protein and a transformant which is introduced with the DNA in a form that allows expression of the protein encoded by the DNA.

Abstract: In a method for producing a target substance by utilizing a microorganism comprising culturing a bacterium belonging to the genus Escherichia in a medium to produce and accumulate the target substance in the medium or cells of the bacterium and collecting the target substance, a strain in which an RMF protein does not function normally in its cell due to, for example, disruption of rmf gene on chromosome, is used to improve production efficiency or production rate of a useful substance such as L-amino acid, protein and nucleic acid.

Abstract: A protein which has a GMP synthetase activity, a DNA which codes for the protein and a transformant which is introduced with the DNA in a form that allows expression of the protein encoded by the DNA.

Abstract: Nucleoside 5′-phosphate ester is produced by culturing a bacterium belonging to the genus Escherichia having an ability to produce nucleoside 5′-phosphate ester, in which ushA gene and aphA gene do not function normally, in a medium to produce and accumulate nucleoside 5′-phosphate ester in the medium, and collecting the nucleoside 5′-phosphate ester from the medium.

Abstract: A nucleic acid substance is efficiently produced by cultivating a microorganism whose repressor protein for purine operon does not function in a normal manner, preferably a strain whose gene encoding the repressor protein on a chromosome (purR) is disrupted, in a culture medium so that the nucleic acid substance should accumulate in the culture medium, and collecting the substance from the culture medium.

Abstract: A protein which has a GMP synthetase activity, a DNA which codes for the protein and a transformant which is introduced with the DNA in a form that allows expression of the protein encoded by the DNA.

Abstract: Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.

Abstract: A coryneform bacterium having high L-lysine productivity is provided by integrating a gene coding for aspartokinase originating from coryneform bacteria with desensitized feedback inhibition by L-lysine and L-threonine, into chromosomal DNA of a coryneform bacterium harboring leaky type homoserine dehydrogenase or a coryneform bacterium deficient in homoserine dehydrogenase gene.