Abstract: There is provided means for analyzing organism-related molecules, dealing with multi item analysis, that are captured according to probe species, and for collecting according to the probe species. A magnetic micro-particle array is fixed with magnets that are configured with magnetic micro-particles in a capillary and with an array of glass beads to which DNA probes of different types from each other are immobilized. A syringe pump and a cross valve are operated to circulate a sample solution in the magnetic micro-particle array, which is reacted with probe DNAs on a glass bead with a probe. Subsequently, a washing solution is introduced to wash inside of the capillary. Next, respective beads are measured for fluorescence intensities. Furthermore a particular bead is collected based on results of fluorescence measurement. Target molecules captured on a surface of the collected bead may be separated by heat-denaturation, which then may be subjected to next analysis.

Abstract: A bead array chip manufacturing process for manufacturing a bead array chip having plural kinds of beads arrived in a predetermined order in, in a container having a plurality of first channels disposed substantially in parallel with each other and a second channel crossing the plurality of first channels, each of the first channels. The process including lowering a capillary inserted in the second channel and sucking and retaining one bead in one end of the capillary, lifting the capillary to position the beads retained in the one end of the capillary in a desired channel of the plurality of first channels, terminating the suction of the capillary, and generating a uniflow of a fluid in the second channel.

Abstract: The present invention provides an apparatus for efficiently transporting or dispensing transport objects including not only particles but also liquid samples. A liquid in a first liquid transport pipe (3) is fed at a liquid feed velocity (V1), and a liquid droplet is formed toward an open end of a second liquid transport pipe (4) disposed with an air gap (11) in between. The particle is released into the liquid droplet, so that the particle is enclosed in the liquid droplet. Suction with a liquid feed velocity (V2) is applied to the inside of the second liquid transport pipe. Since the relationship between V1 and V2 is V1<V2, the liquid droplet, after reaching the open end (10) of the second liquid transport pipe, is sheared off by suction, thereby forming a liquid section in the second liquid transport pipe.

Abstract: Provided is a convenient process of manufacturing a two-dimensional bead array on a chip by disposing a plurality of beads immobilized with a biomolecular probe in a bead array container in a predetermined order. The bead array chip is manufactured by repeating the following operations: a container having a plurality of first channels disposed in parallel with each other and a second channel crossing therewith is retained in a container retaining portion and a capillary is moved through the second channel of the container vertically. The capillary is moved downward to suck and retain, in an end thereof, one bead stored in a storing portion of a bead storing plate and then, moved upward to the position of a desired first channel. Under this state, pure water is fed to the first passage from a water feed system and a water stream is generated by sucking the pure water by a suction pump.

Abstract: Beads having diameters of several tens of micrometers to several millimeters and immobilized with biomolecules are captured by one kind of bead capturing nozzle one by one without fail. Using a bead holding plate having a plurality of wells each of which holds a plurality of the beads and a solution, a vibration generator mounted with a stage to attach the bead holding plate, and a bead capturing nozzle for bead capture connected to a suction pump, only a single bead is captured at the tip of the bead capturing nozzle by inserting the nozzle having an inner diameter smaller than the beads and a negative pressure inside created by the pump into the solution to allow the nozzle to come in contact with the beads in the well, applying a vibration to the bead holding plate by the vibration generator, and withdrawing the bead capturing nozzle into the air.

Abstract: The invention provides a method in which an annular or spiral droplet holder formed of wire is used to hold a droplet in a state of being hung therefrom or being contained therein. A means for moving the droplet holder is added to the droplet holder to enable droplet transfer. To merge two droplets, they are brought into contact. To drip the droplet held by a droplet holder formed of wire, the droplet holder is deformed using an external force. A light path which passes through a droplet is set to enable optical measurement. The present invention enables inexpensive, simple droplet transfer. An inexpensive, simple configuration for handling droplets in the fields of chemical analysis, biochemical analysis, and automatic blood analysis can be realized according to the present invention.

Abstract: There are provided a novel method and technology for arraying micro-particles. Micro-particle trapping capillaries each having an inner diameter smaller than the outer diameter of probe-immobilized micro-particles are prepared. By vacuuming the inside of each micro-particle trapping capillary, only one of the micro-particles is vacuumed onto an opening at the tip thereof and taken out from holders holding a plurality of the micro-particles. The micro-particle vacuumed onto the opening at the tip of each micro-particle trapping capillary is positioned at the opening of the capillary or the edge of each channel provided in a chip, the channels each having an inlet and an outlet with a slightly larger width than the outer diameter of the micro-particle so as to allow passage of only one micro-particle. The micro-particle vacuumed onto the opening at the capillary tip is injected into the capillary from the opening of the capillary or the channel edge of the chip.

Abstract: Beads having diameters of several tens of micrometers to several millimeters and immobilized with biomolecules are captured by one kind of bead capturing nozzle one by one without fail. Using a bead holding plate having a plurality of wells each of which holds a plurality of the beads and a solution, a vibration generator mounted with a stage to attach the bead holding plate, and a bead capturing nozzle for bead capture connected to a suction pump, only a single bead is captured at the tip of the bead capturing nozzle by inserting the nozzle having an inner diameter smaller than the beads and a negative pressure inside created by the pump into the solution to allow the nozzle to come in contact with the beads in the well, applying a vibration to the bead holding plate by the vibration generator, and withdrawing the bead capturing nozzle into the air.

Abstract: There will be provided a chemical reaction device and a chemical reaction apparatus capable of performing transverse liquid movement in a simple structure and at low cost without causing contamination and air bubbles. There will be installed a mechanism for supporting the chemical reaction device in any other position than a center of a turntable which can be rotated, for moving liquid by a centrifugal force due to rotation, and for reversing a direction of the flow path independently of the turntable.

Abstract: In enzymatic reaction carried out batch-wise, loss of the sample cannot be ignored, and according to the conventional technologies aiming at diminishment of the loss of the sample, a long time is required for reactions. In the present invention, the reaction part in which a chemical substance is immobilized is filled with a sample solution, and the sample solution is held between air at both ends for inhibition of mixing with a buffer solution. The sample solution is provided utilizing a sample introduction part, etc.

Abstract: A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel.

Abstract: Provided is a convenient process of manufacturing a two-dimensional bead array on a chip by disposing a plurality of beads immobilized with a biomolecular probe in a bead array container in a predetermined order. The bead array chip is manufactured by repeating the following operations: a container having a plurality of first channels disposed in parallel with each other and a second channel crossing therewith is retained in a container retaining portion and a capillary is moved through the second channel of the container vertically. The capillary is moved downward to suck and retain, in an end thereof, one bead stored in a storing portion of a bead storing plate and then, moved upward to the position of a desired first channel. Under this state, pure water is fed to the first passage from a water feed system and a water stream is generated by sucking the pure water by a suction pump.

Abstract: There is provided means for analyzing organism-related molecules, dealing with multi item analysis, that are captured according to probe species, and for collecting according to the probe species. A magnetic micro-particle array is fixed with magnets that are configured with magnetic micro-particles in a capillary and with an array of glass beads to which DNA probes of different types from each other are immobilized. A syringe pump and a cross valve are operated to circulate a sample solution in the magnetic micro-particle array, which is reacted with probe DNAs on a glass bead with a probe. Subsequently, a washing solution is introduced to wash inside of the capillary. Next, respective beads are measured for fluorescence intensities. Furthermore a particular bead is collected based on results of fluorescence measurement. Target molecules captured on a surface of the collected bead may be separated by heat-denaturation, which then may be subjected to next analysis.

Abstract: In the batch process of enzyme-catalyzed reactions, a sample loss cannot be negligible, but devices according to the prior art aiming at reduction in sample loss cannot help consuming a long period of time in the reaction process. In the present invention, a chemical substance is fixed and a buffer solution is filled in a round-shaped reaction element. A given volume of air is sucked in through an air inlet and a sample solution is sucked in. In addition, a given volume of air is sucked in. Air and the sample solution, is transported sequentially into the reaction element in that order. The reaction element is filled with the sample solution, which is placed between two air layers to prevent it from mixing with the buffer solution. The sample solution between two air layers reciprocates at a given velocity of flow in the reaction element, accelerating high efficiency chemical reactions.

Abstract: The invention provides a solution mixing method by moving relatively at least one DNA array to which probes are fixed to a moving plate with a solution entertained therebetween, while the solution contains a substance of interest which is intended to react with the probes. Such a method can be implemented by an apparatus including a substrate holding plate with at least on substrate and a moving plate (moving along a straight line or revolving), an apparatus including a spinning compartment for spinning about a central axis and a holding plate for holding probes on its surface when being spun along the spinning compartment.

Abstract: There are provided a novel method and technology for arraying micro-particles. Micro-particle trapping capillaries each having an inner diameter smaller than the outer diameter of probe-immobilized micro-particles are prepared. By vacuuming the inside of each micro-particle trapping capillary, only one of the micro-particles is vacuumed onto an opening at the tip thereof and taken out from holders holding a plurality of the micro-particles. The micro-particle vacuumed onto the opening at the tip of each micro-particle trapping capillary is positioned at the opening of the capillary or the edge of each channel provided in a chip, the channels each having an inlet and an outlet with a slightly larger width than the outer diameter of the micro-particle so as to allow passage of only one micro-particle. The micro-particle vacuumed onto the opening at the capillary tip is injected into the capillary from the opening of the capillary or the channel edge of the chip.

Abstract: A capillary array electrophoresis system by which measurement is conducted using a large number of capillaries. The electrophoresis system includes a plurality of capillary array sheets stacked one on top of another wherein end portions of the capillaries at a detection region are arranged two-dimensionally in such a manner as to elute two-dimensionally a sample from the distal end of each capillary. Excitation light is applied to the sample eluted into a buffer solution, and a two-dimensional fluorescent image is picked up by a detector.