Abstract: A dual-bearing reel includes a reel body, a spool rotatably held between side plates, and a brake unit configured to brake the spool. The brake unit includes a cover portion, a first regulator and a second regulator. The cover portion has a first circumferential part and a second circumferential part, and is mounted to the reel body. The first regulator and the second regulator are covered with the cover portion. The first circumferential part has a first opening exposing at least a part of the first regulator therethrough, whereas the second circumferential part has a second opening exposing at least a part of the second regulator therethrough. The reel body has a first restriction part covering the first opening.

Abstract: A dual-bearing reel includes a reel body, a spool rotatably held between side plates, and a brake unit configured to brake the spool. The brake unit includes a cover portion, a first regulator and a second regulator. The cover portion has a first circumferential part and a second circumferential part, and is mounted to the reel body. The first regulator and the second regulator are covered with the cover portion. The first circumferential part has a first opening exposing at least a part of the first regulator therethrough, whereas the second circumferential part has a second opening exposing at least a part of the second regulator therethrough. The reel body has a first restriction part covering the first opening.

Abstract: A GPS loop antenna attached to the front windshield of a vehicle to receive a circularly polarized wave which is improved in reception performance, that is, a loop antenna comprised of a loop-shaped antenna conductor receiving a circularly polarized wave, feed terminals connected to the two ends of the antenna conductor, and a parasitic element positioned near the antenna conductor and made of a conductor independent of the antenna conductor, all arranged on a sheet-like transparent film, wherein a looping line conductor is arranged around the loop antenna on the film. It is sufficient if the total length of the line conductor is about three times the antenna conductor.

Abstract: Probe 1 for detection and quantification of nitric oxide, which comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3?,5?-cyclic monophospate.

Abstract: A bicycle motor control apparatus includes a bicycle crank position detecting device, a bicycle crank position determining section, a stop determining section and a motor control section. The bicycle crank position detecting device detects a position of a bicycle crank. The bicycle crank position determining section determines if the bicycle crank is in a prescribed region including a bottom dead center position based on a detection result from the bicycle crank position detecting device. The stop determining section that determines a stopped state indicative of at least one a bicycle crank stopped state and a bicycle stopped state. The motor control section selectively outputs a motor driving command and stops the output of the motor driving command during a period in which the bicycle crank position determining section determines that the bicycle crank is in the prescribed region and the stop determining section determines that the stopped state exists.

Abstract: It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.

Abstract: A bicycle motor control apparatus includes a bicycle crank position detecting device, a bicycle crank position determining section, a stop determining section and a motor control section. The bicycle crank position detecting device detects a position of a bicycle crank. The bicycle crank position determining section determines if the bicycle crank is in a prescribed region including a bottom dead center position based on a detection result from the bicycle crank position detecting device. The stop determining section that determines a stopped state indicative of at least one a bicycle crank stopped state and a bicycle stopped state. The motor control section selectively outputs a motor driving command and stops the output of the motor driving command during a period in which the bicycle crank position determining section determines that the bicycle crank is in the prescribed region and the stop determining section determines that the stopped state exists.

Abstract: The claimed invention comprises a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a co-activator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule.

Abstract: A method is herein presented for analyzing nucleobases on a single molecular basis (a single molecule detection), which comprises scanning a molecular tip chemically modified with the complementary nucleobase on the nucleobases, and measuring the tunneling currents between the scanned nucleobases and the molecular tips with scanning tunneling microscopy.

Abstract: Probe 1 for detection and quantification of nitric oxide, which comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3?,5?-cyclic monophospate.

Abstract: It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.

Abstract: This application aims to provide a single molecule-format probe for detecting a target-specific ligand easily and accurately as an index of the presence or absence of a signal. The invention is a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, and an N-terminal polypeptide (N-LE) and a C-terminal polypeptide (C-LE) of a luminescent enzyme (LE), which are linked to each end of the ligand-binding molecule, respectively, wherein the N-LE and the C-LE self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule. The ligand-binding molecule is a fusion molecule carrying a ligand-binding domain (LBD) and an LBD-interacting domain or peptide that interacts with the LBD upon binding of the ligand to the LBD.

Abstract: A GPS loop antenna attached to the front windshield of a vehicle to receive a circularly polarized wave which is improved in reception performance, that is, a loop antenna comprised of a loop-shaped antenna conductor receiving a circularly polarized wave, feed terminals connected to the two ends of the antenna conductor, and a parasitic element positioned near the antenna conductor and made of a conductor independent of the antenna conductor, all arranged on a sheet-like transparent film, wherein a looping line conductor is arranged around the loop antenna on the film. It is sufficient if the total length of the line conductor is about three times the antenna conductor.

Abstract: A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order.

Abstract: A method for analyzing an organelle-localized protein, which enables one to determine whether or not a test protein localizes to an organelle, comprising the steps of: (a) a step of introducing a fusion peptide (a), which comprises one half-peptide of an intein, one half-peptide of a fluorescent protein and an organelle-targeting signal peptide, into a eukaryotic cell; (b) a step of introducing a test protein bound to a fusion peptide (b), which comprises the other half-peptide of the fluorescent protein and the other half-peptide of the intein, into the eukaryotic cell; and (c) a step of detecting fluorescence signal emitted by the fluorescent protein, and a material for analysis to be used in such method are provided.

Abstract: A loop antenna able to be mounted on the rear window of a vehicle provided with a defogger, that is, a loop antenna to be mounted on a rear window provided with a defogger having electrodes arranged in the vertical direction at the two ends and a plurality of electrical heating wires bridging the electrodes in the horizontal direction, wherein an antenna element forming a loop is made polygonal in shape, two power feed terminals of the loop antenna are provided at positions a predetermined distance away from the midpoint of the bottom of the antenna element, constituted by one side, in the vertical direction, a distance between the power feed terminals and bottom is formed smaller than the distance between adjoining electrical heating wires of the defogger, and the loop antenna is mounted on the rear window between the adjoining electrical heating wires. The polygonal shape may be a triangular shape.

Abstract: A probe for detection and quantification with high accuracy in a noninvasive manner as to where and when inositol-1,4,5-trisphosphate is generated in living cells, and a method for detecting and quantifying inositol-1,4,5-trisphosphate using the probe.

Abstract: A method is herein presented for analyzing nucleobases on a single molecular basis (a single molecule detection), which comprises scanning a molecular tip chemically modified with the complementary nucleobase on the nucleobases, and measuring the tunneling currents between the scanned nucleobases and the molecular tips with scanning tunneling microscopy.

Abstract: The present invention provides ligand detection means capable of exhibiting two-dimensional information (wavelength and intensity of luminescent signal) responding to multiple signals triggered by a ligand via a target protein, while taking advantage of the merit of the single-molecule-format bioluminescent probe. The present invention could provide a luminescent probe set which is comprised of a fusion protein comprising a ligand recognition protein and a molecular recognition domain to which the ligand recognition protein bind upon conformational change, wherein the fusion protein is sandwiched between split Lighting Enzyme fragments, the probe set can emit multiple luminescence by utilizing Lighting Enzymes emitting lights with multiple wavelength, wherein these multiple components can be tandemly arranged by using C-terminal fragment of the Lighting Enzyme.

Abstract: A probe for detecting an agonist or an antagonist to a nuclear receptor, in which, at least, a ligand-recognition site containing a ligand-binding domain of the nuclear receptor is connected with a binding-responsive site containing a peptide chain that specifically binds to a coactivator-binding site in the ligand-binding domain by a flexible linker to construct a fusion structure [ligand-recognition site/linker/binding-responsive site], and two reporters are connected with the respective ends of the fusion structure.