Abstract: The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

Abstract: Probe 1 for detection and quantification of nitric oxide, which, comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3?,5-cyclic monophospate.

Abstract: As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.

Abstract: As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.

Abstract: When configuring a film antenna for receiving a circular polarized wave, at least one loop antenna is formed on a transparent plastic film and, at the same time, a non-powered element constituted by a wire-shaped conductor independent from the antenna conductor configuring the loop is arranged near this loop antenna. The non-powered element arranged on the side of the loop antenna is configured by a first part and a second part. The first part is made close to the loop antenna in a substantially parallel state. When a monopole antenna is used in place of the loop antenna, by combining this with a wire-shaped conductor orthogonal to this, it becomes possible to receive a circular polarized wave by a configuration providing a power transfer part between the two. It is also possible to configure a composite antenna by mounting another antenna on the transparent plastic film. This antenna can be used as an antenna of a navigation system.

Abstract: A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order.

Abstract: A rod antenna with a good reception sensitivity using a conductive body of a vehicle or a heater element of a defogger as a reflector, that is, a rod antenna to be mounted at a conductive outside surface of a vehicle or at an outside surface of a rear window of a vehicle where heater elements of a defogger are arranged in parallel, provided with a rod-shaped antenna element provided with a predetermined length and a holding member comprised of a base member for holding the antenna element and an antenna support member, the holding member holding the antenna element so that it is separated a predetermined distance from the outside surface so that the outside surface becomes a reflector for the antenna element.

Abstract: A loop antenna able to be mounted on the rear window of a vehicle provided with a defogger, that is, a loop antenna to be mounted on a rear window provided with a defogger having electrodes arranged in the vertical direction at the two ends and a plurality of electrical heating wires bridging the electrodes in the horizontal direction, wherein an antenna element forming a loop is made polygonal in shape, two power feed terminals of the loop antenna are provided at positions a predetermined distance away from the midpoint of the bottom of the antenna element, constituted by one side, in the vertical direction, a distance between the power feed terminals and bottom is formed smaller than the distance between adjoining electrical heating wires of the defogger, and the loop antenna is mounted on the rear window between the adjoining electrical heating wires. The polygonal shape may be a triangular shape.

Abstract: A convenient and commonly applicable method for the specific detection of a nucleic acid with an arbitrary sequence is provide. This method comprises attaching at least a nucleic acid single strand to an electrode, bringing the thus-obtained modified electrode in contact with a solution containing the analyte single-stranded nucleic acid, and measuring the redox reaction of the redox marker.

Abstract: The present invention provides a pair of probes for analyzing protein-protein interactions, which comprises a probe A containing at least an N-terminal half polypeptide of split Renilla luciferase, and a probe B containing at least the remaining C-terminal half polypeptide of split Renilla luciferase.

Abstract: A probe for protein—protein interaction analysis suitable for analyzing protein—protein interactions of various proteins with high accuracy and in a simple manner and a method for analyzing interaction of two proteins by using the probe. With the probe, protein splicing is caused by protein—protein interaction, and a physicochemically or biochemically detectable protein is regenerated.

Abstract: The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

Abstract: As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.

Abstract: As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.

Abstract: As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.

Abstract: A convenient and commonly applicable method for the specific detection of a nucleic acid with an arbitrary sequence is provide. This method comprises attaching at least a nucleic acid single strand to an electrode, bringing the thus-obtained modified electrode in contact with a solution containing the analyte single-stranded nucleic acid, and measuring the redox reaction of the redox marker.

Abstract: A method for analyzing an organelle-localized protein, which enables one to determine whether or not a test protein localizes to an organelle, comprising the steps of: (a) a step of introducing a fusion peptide (a), which comprises one half-peptide of an intein, one half-peptide of a fluorescent protein and an organelle-targeting signal peptide, into a eukaryotic cell; (b) a step of introducing a test protein bound to a fusion peptide (b), which comprises the other half-peptide of the fluorescent protein and the other half-peptide of the intein, into the eukaryotic cell; and (c) a step of detecting fluorescence signal emitted by the fluorescent protein, and a material for analysis to be used in such method are provided.

Abstract: When configuring a film antenna for receiving a circular polarized wave, at least one loop antenna is formed on a transparent plastic film and, at the same time, a non-powered element constituted by a wire-shaped conductor independent from the antenna conductor configuring the loop is arranged near this loop antenna. The non-powered element arranged on the side of the loop antenna is configured by a first part and a second part. The first part is made close to the loop antenna in a substantially parallel state. When a monopole antenna is used in place of the loop antenna, by combining this with a wire-shaped conductor orthogonal to this, it becomes possible to receive a circular polarized wave by a configuration providing a power transfer part between the two. It is also possible to configure a composite antenna by mounting another antenna on the transparent plastic film. This antenna can be used as an antenna of a navigation system.

Abstract: As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.

Abstract: A probe for protein-protein interaction analysis applicable to various proteins, which enables simple analysis in high accuracy, and a method for analyzing interaction of two proteins is provided. Protein splicing from the probe is caused by protein-protein interaction, and a physiochemically or biochemically detectable protein is regenerated.