Abstract: The invention provides a process for producing low-carbon olefins by gaseous phase catalytic cracking of heavy oil with multi-stages in milliseconds, comprising: a high-efficiency atomizing nozzle sprays the preheated heavy oil into an upper portion of the downflow modification reaction tube, the produced oil mist is mixed with a high temperature heat carrier flowing downward from a return controller for pyrolysis, and then the pyrolysis products are subject to a gas-solid separation at the bottom of the downflow modification reaction tube; then the coked heat carrier obtained by the separation enters into a lower portion of a modification regeneration reactor to conduct a regeneration reaction, the obtained regeneration gas and the high temperature heat carrier are subject to a gas-solid separation on the top of the modified regeneration reactor, then the high temperature heat carrier returns to a top of the downflow reaction tube to participate in circulation, the regeneration gas is subject to heat exchang

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5?-end extension primer, wherein the single-stranded 5?-end extension primer comprises a sequencing platform adaptor sequence of a 5? end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3?-end adaptor sequence to the 3? end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

Abstract: The present invention provides, among other things, compositions and methods for CNS delivery of lysosomal enzymes for effective treatment of lysosomal storage diseases. In some embodiments, the present invention includes a stable formulation for direct CNS intrathecal administration comprising a heparan N-sulfatase (HNS) protein, salt, and a polysorbate surfactant for the treatment of Sanfilippo Syndrome Type A.

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3?-terminal and the 5?-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3?-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Abstract: A computer system may record one or more service assignment outcomes of the first service provider over the service session interval, where each of the one or more service assignment outcomes are associated with the location of the first service provider at a time when that service assignment outcome occurred. The computer system may determine satisfaction score of the service session interval based at least in part on the recorded one or more service assignment outcomes. A remedial action may be performed in response to a determination of the satisfaction score not satisfying a threshold.

Abstract: The present disclosure discloses a method and apparatus for measuring an angular resolution of a multi-beam lidar. The method comprises: acquiring, when a checkerboard calibration plate is scanned by a multi-beam lidar, an image of the checkerboard calibration plate photographed by a camera; identifying a checkerboard in the image, and determining a physical length characterized by a unit pixel of the image based on shape and length information of each checker in the checkerboard calibration plate; determining a center light spot and a light spot pair in the image; determining an angle between a laser beam corresponding to each light spot of the light spot pair and a laser beam corresponding to the center light spot based on the physical length characterized by the unit pixel, the center light spot and the light spot pair; and determining the angular resolution of the multi-beam lidar based on the determined angle.

Abstract: A method is provided for creating a volume graphic via a catheter for introduction into a vessel which has an ultrasound unit, which is embodied to transmit ultrasound, to measure an echo of the transmitted ultrasound, and to create image data for a two-dimensional cross-section of the vessel on the basis of the measured echo. The method involves creating image data of a cross-section with the ultrasound unit at a plurality of different positions on a movement track of the catheter, and, in addition to the image data, creating position data for the current position of the ultrasound unit with a localization device. The method further involves creating the volume graphic from the image data of all cross-sections in which the cross-sections are arranged relative to one another in accordance with the position data.

Abstract: The present invention relates to a patch antenna having programmable frequency and polarization comprising the first metal covering layer, the dielectric layer, the second metal covering layer and four metallized through-holes, each of which are disposed sequentially from top to bottom, wherein the first metal covering layer comprises the feeding line and the radiating patch; the feeding line comprises the micro-strip line, which can be connected to the outer feeding port; the micro-strip line is connected to one side of the radiating patch through the high-resistance line; the radiating patch is a square-shaped metal patch; a gap is etched near the other side of the radiating patch, namely, the radiating edge; the gap is parallel to the radiating edge.

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5?-end extension primer, wherein the single-stranded 5?-end extension primer comprises a sequencing platform adaptor sequence of a 5? end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3?-end adaptor sequence to the 3? end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

Abstract: Provided herein are compositions, methods, and kits for enriching for one or more nucleic acid sequences of interest in a sample. The methods include providing a circular ligase, one or more 5? hook probes and/or one or more 3? hook probes and contacting the sample comprising the nucleic acids with the circular ligase and one or more 5? hook probes and/or one or more 3? hook probes under conditions to allow the hook probes to selectively bind to the one or more nucleic acid sequences of interest, and under conditions to form one or more hook products, each hook product comprising the hook probes and the one or more nucleic acid sequences of interest.

Abstract: The present invention relates to a parallel-feeding, dual-band and dual-polarized base station antenna comprising a radiating patch layer, four F-shaped metal strips which are perpendicular to the radiating patch layer and orthogonal to each other, and a feeding layer, which is sequentially disposed from top to bottom, wherein the radiating patch layer comprises the first metal covering layer and the first dielectric layer; wherein the first metal covering layer is square-shaped and an isosceles triangle having the same size is cut from each corner of the square; wherein the four F-shaped metal strips work as the extended part of the feeding layer to couple-feed the radiating patch layer; the feeding layer comprises the first metal feed-line layer, the second dielectric layer, the metal floor layer, the third dielectric layer and the second metal feed-line layer, which are sequentially disposed from top to bottom.

Abstract: The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library.

Abstract: A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library.

Abstract: The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5?-X-Y-3?, and the sequence composition of the downstream random primers is 5?-P-Y?-X?-close-3?, wherein Y and Y? are random sequences, X is all or part of sequences of a sequencing platform 5? end adaptor, X? is all or part of sequences of a sequencing platform 3? end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

Abstract: The present invention relates to a patch antenna having programmable frequency and polarization comprising the first metal covering layer, the dielectric layer, the second metal covering layer and four metallized through-holes, each of which are disposed sequentially from top to bottom, wherein the first metal covering layer comprises the feeding line and the radiating patch; the feeding line comprises the micro-strip line, which can be connected to the outer feeding port; the micro-strip line is connected to one side of the radiating patch through the high-resistance line; the radiating patch is a square-shaped metal patch; a gap is etched near the other side of the radiating patch, namely, the radiating edge; the gap is parallel to the radiating edge.