Abstract: To provide a scaffold for tissue engineering which consists of a bioabsorbable polymer material to be absorbed in a biotissue, and holds strength of the whole scaffold while having a porosity proper for culturing cells inside thereof as well, a method for producing the scaffold for tissue engineering includes steps of dissolving a bioabsorbable polymer material with an organic solvent, drying the solution so as to produce a porous bioabsorbable polymer material having a porosity of 50 to 99%, covering the porous bioabsorbable polymer material with a bioabsorbable polymer material having a thickness of 0.01 to 5 mm, pores of 10 to 3000 ?m diameter, a fracture strength of 0.05 to 0.15 MPa, and a volume of 15 to 90% with respect to the whole scaffold.

Abstract: To provide a bone tissue regeneration sheet having a cortical bone tissue layer of 200 ?m or more in thickness, the bone tissue regeneration sheet is produced by seeding chondroblast or stem cells for differentiating to chondrocyte on one side of a porous bioabsorbable polymer sheet, taking the seeded porous body into a culture medium, applying centrifugal force of 100 to 1000 G to the culture medium for a predetermined time so as to aggregate the chondroblast or the stem cells for differentiating to chondrocyte, culturing the aggregated cells in a culture medium not containing serum but containing one or two or more kinds selected from ascorbic acid, an ascorbic acid derivative, and dexamethasone without applying centrifugal force to form a chondrocyte layer having a thickness of 200 ?m or more.

Abstract: For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 ?m and an average hole diameter of 50 to 1500 ?m, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force.