Patents by Inventor Yuiko Sahara

Yuiko Sahara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Calcium-binding photoprotein
    Patent number: 10156557
    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: December 18, 2018
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Sahara
  • Process for production of recombinant proteins as a soluble form
    Patent number: 9738692
    Abstract: A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: August 22, 2017
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Sahara
  • Substituted imidazo[1,2-A]pyrazines for measuring a transcription activity
    Patent number: 9645128
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Grant
    Filed: August 14, 2015
    Date of Patent: May 9, 2017
    Assignees: JNC CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Satoshi Inouye, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • COELENTERAZINE ANALOGS AND MANUFACTURING METHOD THEREOF
    Publication number: 20150344936
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Application
    Filed: August 14, 2015
    Publication date: December 3, 2015
    Applicants: TOKYO INSTITUTE OF TECHNOLOGY, JNC CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA, Rie IIMORI, Takamitsu HOSOYA
  • Coelenterazine analogs and manufacturing method thereof
    Patent number: 9151739
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Grant
    Filed: May 21, 2014
    Date of Patent: October 6, 2015
    Assignees: JNC CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Satoshi Inouye, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • Coelenterazine analogues and coelenteramide analogues
    Patent number: 9075058
    Abstract: Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: July 7, 2015
    Assignees: JNC CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • Coelenterazine analogues and coelenteramide analogues
    Patent number: 9056840
    Abstract: Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.
    Type: Grant
    Filed: July 7, 2014
    Date of Patent: June 16, 2015
    Assignees: JNC CORPORATION, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES
    Publication number: 20150153344
    Abstract: Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
    Type: Application
    Filed: January 30, 2015
    Publication date: June 4, 2015
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM
    Publication number: 20150087047
    Abstract: A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.
    Type: Application
    Filed: September 3, 2014
    Publication date: March 26, 2015
    Applicant: JNC CORPORATION
    Inventors: Satoshi INOUYE, Yuiko SAHARA
  • Coelenterazine analogues and coelenteramide analogues
    Patent number: 8883432
    Abstract: Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.
    Type: Grant
    Filed: December 3, 2013
    Date of Patent: November 11, 2014
    Assignees: JNC Corporation, National University Corporation Tokyo Medical and Dental University
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • CALCIUM-BINDING PHOTOPROTEIN
    Publication number: 20140329250
    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.
    Type: Application
    Filed: May 30, 2014
    Publication date: November 6, 2014
    Inventors: Satoshi INOUYE, Yuiko SAHARA
  • COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES
    Publication number: 20140316137
    Abstract: Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.
    Type: Application
    Filed: July 7, 2014
    Publication date: October 23, 2014
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • Process for production of recombinant proteins as a soluble form
    Patent number: 8853374
    Abstract: A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.
    Type: Grant
    Filed: September 18, 2007
    Date of Patent: October 7, 2014
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara
  • COELENTERAZINE ANALOGS AND MANUFACTURING METHOD THEREOF
    Publication number: 20140296521
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Application
    Filed: May 21, 2014
    Publication date: October 2, 2014
    Inventors: Satoshi INOUYE, Yuiko SAHARA, Rie IIMORI, Takamitsu HOSOYA
  • Calcium-binding photoprotein
    Patent number: 8772452
    Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.
    Type: Grant
    Filed: May 10, 2010
    Date of Patent: July 8, 2014
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara
  • Coelenterazine analogs and manufacturing method thereof
    Patent number: 8765921
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Grant
    Filed: August 22, 2013
    Date of Patent: July 1, 2014
    Assignees: JNC Corporation, Tokyo Institute of Technology
    Inventors: Satoshi Inouye, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES
    Publication number: 20140120538
    Abstract: Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
    Type: Application
    Filed: December 3, 2013
    Publication date: May 1, 2014
    Applicants: National University Corporation Tokyo Medical and Dental University, JNC Corporation
    Inventors: SATOSHI INOUYE, YUIKO SAHARA, TAKAMITSU HOSOYA
  • Coelenterazine analogues and coelenteramide analogues
    Patent number: 8642281
    Abstract: Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
    Type: Grant
    Filed: April 5, 2011
    Date of Patent: February 4, 2014
    Assignees: JNC Corporation, National University Corporation Tokyo Medical and Dental University
    Inventors: Satoshi Inouye, Yuiko Sahara, Takamitsu Hosoya
  • COELENTERAZINE ANALOGS AND MANUFACTURING METHOD THEREOF
    Publication number: 20140024058
    Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).
    Type: Application
    Filed: August 22, 2013
    Publication date: January 23, 2014
    Applicants: Tokyo Institute Of Technology, JNC Corporation
    Inventors: Satoshi INOUYE, Yuiko Sahara, Rie Iimori, Takamitsu Hosoya
  • Fusion protein having luminescence activity
    Patent number: 8569003
    Abstract: The fusion protein comprising (1) a first region comprising the amino acid sequence of SEQ ID NO: 18 and (2) a second region comprising an amino acid sequence for a polypeptide containing at least one cysteine residue for binding to other useful compound via the thiol group can be modified by chemical modification, and thus has a high catalytic ability for a luminescence activity and is highly available for general purposes.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: October 29, 2013
    Assignee: JNC Corporation
    Inventors: Satoshi Inouye, Yuiko Sahara, Junichi Sato